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| Abreu,JT; Mourão,MM; Santos,CE; Veloso,CJM; Resende,JS; Flatschart,RB; Folgueras-Flatschart,AV; Júnior,SN; Santoro,MM; Mendes,ACR; Franco,GR; Silva,A; Campos,AB; Fernandez,S. |
| Avian infectious bronchitis virus (IBV) isolates have been widely characterized by reverse transcription followed by polymerase chain reaction and DNA sequencing. In present study, these techniques were applied to three viral genomic regions comprising the complete and/or a partial S1 segment, S2 and nucleocapsid genes. DNA sequences from viral isolates obtained from 1972 to 1989 and from 2006 to 2008 were compared. High similarity (>90%) was observed among some of the genomic segments, including S1 hypervariable region, which could suggest a common origin or ancestry. DNA sequences from S2 and N protein genes obtained from different infected tissues of the same flock were analyzed, and a clear segregation between respiratory and intestinal tract was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Infectious bronchitis virus; Genotyping; Spike glycoprotein; Nucleocapsid; Phylogenetic tree. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2010000200005 |
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