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Antinociceptive activity of sulfated carbohydrates from the red algae Bryothamnion seaforthii (Turner) Kütz. and B. triquetrum (S.G. Gmel.) M. Howe BJMBR
Viana,G.S.B.; Freitas,A.L.P.; Lima,M.M.L.; Vieira,L.A.P.; Andrade,M.C.H.; Benevides,N.M.B..
We report the antinociceptive activity, determined by the writhing, formalin and hot-plate tests in mice, of crude (F0/60), lectin and carbohydrate fractions isolated by ammonium sulfate precipitation (0 to 60%) from Bryothamnion seaforthii and B. triquetrum, species of red algae. Not only fraction F0/60 but also lectins from both species significantly inhibited acetic acid-induced abdominal contractions after intraperitoneal or oral administrations. In the formalin test, lectins (1 and 5 mg/kg, ip, and 5 to 20 mg/kg, po) inhibited the 1st and 2nd phases (5 and 20 min, respectively), but the effect occurred predominantly on the 2nd phase. The effects of the lectins were totally or partially reversed by naloxone (2 mg/kg, sc) in the 1st and 2nd phases,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Red algae; Bryothamnion seaforthii; Bryothamnion triquetrum; Carbohydrates; Antinociceptive effect.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000600012
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The alga Bryothamnion seaforthii contains carbohydrates with antinociceptive activity BJMBR
Vieira,L.A.P.; Freitas,A.L.P.; Feitosa,J.P.A.; Silva,D.C.; Viana,G.S.B..
Bryothamnion seaforthii, a red alga common to the Northeastern coast of Brazil, was used to prepare the protein fraction F0/60 by ammonium sulfate precipitation. The chromatography of F0/60 on DEAE-Sephadel column resulted in two lectin fractions, PI and PII, which have antinociceptive properties in rodents. We determined the antinociceptive activity of the PII fraction and of a carbohydrate-containing fraction (CF) in mice. The CF was prepared from the dried algae, after digestion with 100 mM sodium acetate, pH 6.0, containing 5 mM cysteine, EDTA and 0.4% papain, at 60ºC. A 10% cetylpyridinium chloride was added to the filtrate, and the precipitate was dissolved with 2 M NaCl:ethanol (100:15, v/v) followed by the carbohydrate precipitation with ethanol....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Algae; Antinociceptive activity; Carbohydrate; Bryothamnion seaforthii.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700017
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Antinociceptive properties in mice of a lectin isolated from the marine alga Amansia multifida Lamouroux BJMBR
Neves,S.A.; Freitas,A.L.P.; Souza,B.W.S.; Rocha,M.L.A.; Correia,M.V.O.; Sampaio,D.A.; Viana,G.S.B..
The antinociceptive effects of a lectin (LEC) isolated from the marine alga Amansia multifida were determined in Swiss mice. The LEC (1, 5, and 10 mg/kg) inhibited acetic acid-induced abdominal writhings in a dose-dependent manner after intraperitoneal or oral administration. A partial but significant inhibition of writhings was observed after the combination of LEC (10 mg/kg) with avidin (1 mg/kg), a potent inhibitor of the hemmaglutinant activity of the lectin. However, total writhing inhibition was demonstrable in the group of mice treated with LEC plus mannose (1 mg/kg), as compared to LEC alone or to control groups. Furthermore, avidin and mainly mannose also play a role in antinociception, somehow facilitating the interaction of LEC with its active...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Red algae; Amansia multifida; Lectin; Antinociceptive effects; Mannose-specific lectin.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100016
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Evaluation of biofilm production by Pseudomonas Aeruginosa isolates recovered from cystic fibrosis and non-cystic fibrosis patients BJM
Perez,L.R.R.; Costa,M.C.N.; Freitas,A.L.P.; Barth,A.L..
Cystic fibrosis (CF) patients typically suffer of persistent and recurrent lung infections caused by Pseudomonas aeruginosa that many times possess ability for the biofilm production. Here, biofilm production among P. aeruginosa isolates recovered from sputum of CF and non-CF patients was evaluated. Most isolates were biofilm-producing independently of the patient's condition.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Biofilm; Cystic fibrosis.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200011
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