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| Rojas,Mariana; Venegas,Felipe; Servey,Jean Luc; Guillomot,Michel. |
| La Biología del Desarrollo y la Biotecnología han avanzado enormemente en los últimos años. Los estudios que se están realizando actualmente con nuevas técnicas moleculares, nos conducen a una nueva era en el diagnóstico y en el tratamiento de muchas enfermedades congénitas. Actualmente, se ha logrado aplicar los conocimientos derivados de ella, con incipiente éxito, en los aspectos productivos y también en la terapia de distintas enfermedades del Hombre y de otros animales y, por último, existen importantes perspectivas en la protección de especies amenazadas. En este Review, se analizan algunos de los aspectos técnicos más interesantes de la clonación, producción de quimeras y obtención de células pluripotenciales, como también los objetivos y... |
| Tipo: Journal article |
Palavras-chave: Clonación; Quimeras; Células pluripotenciales. |
| Ano: 2004 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022004000400018 |
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| Rojas,Mariana; Venegas,Felipe; Montiel,Enrique; Servely,Jean Luc; Vignon,Xavier; Guillomot,Michel. |
| The somatic cloning by transfer of the nuclei of differentiated adult cells to previously enucleated oocytes is a promising technique for the production of embryos of high genetic value. The better mastering of somatic cloning gives us the possibility to produce embryos from endangered species. The huemul is an Andean native deer, that has been declared an endangered species, it holds a great patrimonial value and it is a Chilean national emblem. In Chile the huemul has the status of protected animal on thirteen Parks and National Reserves managed by Corporacion Nacional Forestal (CONAF). This protection, however, is considered insufficient due to the few geographical connections between the different protected areas. Furthermore, a great proportion of... |
| Tipo: Journal article |
Palavras-chave: Huemul; Interspecific cloning; Endangered species. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022005000400008 |
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| Venegas,Felipe; Guillomot,Michel; Vignon,Xavier; Servely,Jean-Luc; Audouard,Chistophe; Montiel,Enrique; Le Bourhis,Daniel; Perón,Sandrine; Soto,Pilar; Rojas,Mariana. |
| The objective of this project is to produce pudu embryos, obtained by means of fibroblast transfer taken from the ear of one animal, to the oocytes of a domestic ruminant such as cattle. In further work, the pudu embryos will be transferred to the uterus of synchronized recipient females of another species. Biopsies 1mm in size were obtained from the outer rim of the ear of two male pudu deer belonging to the Buin Zoological park, Santiago, Chile. The cell lines have been established and kept according to protocols used in cattle. The oocytes are obtained through a puncture in the oocyte-cumulus complex (OCC) from cow ovaries recovered from the butcher. Each oocyte is enucleated and fused with an isolated fibroblast which is inserted beneath the pellucid... |
| Tipo: Journal article |
Palavras-chave: Pudu deer; Cervidae; Ruminant; Cloning. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000300026 |
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