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Slaughterhouse blood as a perfusate for studying myocardial function under ischemic conditions BJMBR
Bendjelid,K.; Canet,E.; Gasche,Y.; Andre-Fouet,X.; Revel,D.; Janier,M..
Metabolic studies using the in vitro non-recirculating blood-perfused isolated heart model require large volumes of blood. The present study was designed to determine whether heterologous pig blood collected from a slaughterhouse can be used as perfusate for isolated pig hearts perfused under aerobic and constant reduced flow conditions. Eight isolated working pig hearts perfused for 90 min at a constant flow of 1.5 ml g-1 min-1 with non-recirculated blood diluted with Krebs-Henseleit bicarbonate buffer at a hematocrit of 23% were compared to eight hearts subjected to the same protocol but perfused only with Krebs-Henseleit bicarbonate buffer solution. Hearts were paced at 100 bpm and subjected to aerobic perfusion at 38ºC. Hearts were weighed before...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Myocardium; Ischemia; Erythrocytes; Edema; Reperfusion injury.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100006
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