Data/hora: 26/07/2024 22:43:23

Provedor de dados: 	Scientia Agricola	
País: 	Brazil	
Título: 	Marker assisted identification of tospovirus resistant tomato genotypes in segregating progenies	
Autores: 	Nascimento,Ildon Rodrigues do; Maluf,Wilson Roberto; Figueira,Antônia Reis; Menezes,Cícero Beserra; Resende,Juliano Tadeu Vilela de; Faria,Marcos Ventura; Nogueira,Douglas Willian.	
Data: 	2009-06-01	
Ano: 	2009	
Palavras-chave: 	Lycopersicon esculentum; Sw-5; Vírus resistance; Molecular marker.	
Resumo: 	The SCAR (Sequence Characterized Amplified Region) 'Sw-421' molecular marker is located at 1.0 cM from the Sw-5 allele, originated from Lycopersicon peruvianum (L.), which confers resistance to the tomato spotted wilt virus (TSWV). However, it had not been tested yet in advanced tomato populations. The goal of this study was to distinguish resistant homozygotes (Sw-5/Sw-5) and heterozygotes (Sw-5/Sw-5+) from susceptible (Sw-5+/Sw-5+) plants in crossing populations with the Stevens cultivar and advanced backcrossing populations by using 'Sw421' SCAR marker. The amplification of 940 bp and 900 bp bands characterized the resistant homozygotes and susceptible controls, respectively. A two band pattern (900 bp and 940 bp) was observed in heterozygote genotypes (Sw-5/Sw-5+), which confirmed the co-dominant inheritance mechanism of the marker. Fifty seven plants from the isogenic progenies were characterized based on bands pattern: 18 plants (31.6%) were identified as resistant homozygotes, 8 plants (14.0%) as resistant heterozygotes and 31 plants (54.4%) were characterized as susceptible. The SCAR 'Sw-421' marker is an important tool for selection and pyramid resistance alleles, mainly when other resistance sources to the TSWV are available, such as the Rey de los Tempranos source.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000300003	
Editor: 	São Paulo - Escola Superior de Agricultura "Luiz de Queiroz"	
Relação: 	10.1590/S0103-90162009000300003	
Formato: 	text/html	
Fonte: 	Scientia Agricola  v.66 n.3 2009	
Fonte: 	info:eu-repo/semantics/openAccess