Data/hora: 27/07/2024 01:55:41

Provedor de dados: 	5	
País: 	France	
Título: 	Molecular identification and localization of filamentous symbiotic bacteria associated with the hydrothermal vent annelid Alvinella pompejana	
Autores: 	Cary, S Craig; Cottrell, Matthew T; Stein, Jeffrey L; Camacho, Francisco; Desbruyeres, Daniel.	
Data: 	1997-03	
Ano: 	1997	
Resumo: 	Alvinella pompejana is a polychaetous annelid that inhabits high temperature environments associated with active deep-sea hydrothermal vents along the East Pacific Rise. A unique and diverse epibiotic microflora with a prominent filamentous morphotype is found associated with the worm's dorsal integument. A previous study established the taxonomic positions of two epsilon proteobacterial phylotypes, 13B and 5A, which dominated a clone library of 16S rRNA genes amplified by PCR from the epibiotic microbial community of an A. pompejana specimen. In the present study deoxyoligonucleotide PCR primers specific for phylotypes 13B and 5A were used to demonstrate that these phylotypes are regular features of the bacterial community associated with A. pompejana. Assaying of other surfaces around colonies of A. pompejana revealed that phylotypes 13B and 5A are not restricted to A. pompejana. Phylotype 13B occurs on the exterior surfaces of other invertebrate genera and rock surfaces, and phylotype 5A occurs on a congener, Alvinella caudata. The 13B and 5A phylotypes were identified and localized on A. pompejana by in situ hybridization, demonstrating that these two phylotypes are, in fact, the prominent filamentous bacteria on the dorsal integument of A. pompejana. These findings indicate that the filamentous bacterial symbionts of A. pompejana are epsilon Proteobacteria which do not have an obligate requirement for A. pompejana.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/00336/44734/44830.pdf

http://archimer.ifremer.fr/doc/00336/44734/	
Editor: 	Amer Soc Microbiology	
Formato: 	application/pdf	
Fonte: 	Applied And Environmental Microbiology (0099-2240) (Amer Soc Microbiology), 1997-03 , Vol. 63 , N. 3 , P. 1124-1130	
Fonte: 	1997, American Society for Microbiology

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