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| Maciel,L.F.; Magalhães,C.G.; Moraes,C.M.; Rosa,M.C.; Mendonça,M.; Moreira,A.N.; Dummer,L.A.; Finger,P.F.; Ferreira,M.R.A.; Leite,F.P.L.; Conceição,F.R.. |
| ABSTRACT The strangles is an infectious disease that affects horses from all ages and causes important economic losses in the equine-related business. The aim of this work was to evaluate the immunogenicity of the recombinant M protein from Streptococcus equi (rSeM) co-administered with the recombinant heat-labile enterotoxin B subunit from Escherichia coli (rLTB) in mice and horses. A total of 72 female Balb-c mice were divided into eight groups and 18 horses were divided into six groups. The animals were inoculated by intramuscular (IM) or intranasal (IN) routes with different treatments of rSeM, rLTB and/or Al(OH)3. The results obtained in both species, independent of administration routes, demonstrated that rSeM + rLTB had higher levels of specific... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Garrotilho; Streptococcus equi; RSeM e rLTB. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000501351 |
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| Moraes,C.M.; Conceição,F.R.; Rocha,A.S.R.; Santos Júnior,A.G.; Ribas,L.M.; Vargas,A.P.C.; Nogueira,C.E.W.; Gil-Turnes,C.; Leite,F.P.L.. |
| Strangles is an economically important horse disease caused by Streptococcus equi subsp. equi. The diagnosis can be confirmed either directly by bacterial isolation and PCR or by ELISA, which is an indirect method based on the detection of serum antibodies. The aim of this study was to clone, express and characterize the SeM protein of Streptococcus equi subsp. equi, evaluate its use as antigen in indirect ELISA and determine its performance to distinguish sera of negative, vaccinated and positive animals. This was initially performed by cloning the gene encoding the SeM protein and its expression in Escherichia coli. Subsequently, the protein produced was characterized and used as antigen in ELISA. Serum samples for evaluation were taken from 40 negative... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Strangles Streptococcus equi; SeM ELISA. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000401015 |
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| Ribas,L.M.; Rosa,M.C.; Nogueira,C.E.W.; Finger,I.S.; Cunha,R.C.; Leite,F.P.L.. |
| RESUMO Este trabalho relata o desenvolvimento e a avaliação de um ensaio imunoenzimático (ELISA) como ferramenta auxiliar no controle da adenite equina. Foi avaliada a presença de anticorpos anti-Streptococcus equi subsp. equi em equinos com doença clínica de garrotilho, portadores assintomáticos e potros vacinados. Equinos doentes demonstraram absorbâncias médias superiores (P<0,05) às médias observadas nas demais categorias examinadas. Equinos portadores assintomáticos apresentaram valores médios de absorbância superiores (P<0,05) aos animais com cultura negativa. Logo após a vacinação, potros apresentaram elevação nos níveis de anticorpos, seguida de um decréscimo nos níveis 90 dias após a segunda vacinação. O “Cell ELISA” foi eficiente para a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Garrotilho; Streptococcus equi; ELISA; Portador assintomático. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000100020 |
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