Consulta: creatorFacets:"Lemos,M.V.F."
Registros recuperados: 4
Data/hora: 27/07/2024 03:03:50

Avaliação da reação em cadeia da polimerase e do isolamento bacteriológico convencional na detecção de Salmonella Dublin em amostras de fezes de bezerros infectados experimentalmente
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Silva,D.G.; Silva,D.R.; Silva,P.R.L.; Cícero,E.A.S.; Ferraz,A.L.J.; Lemos,M.V.F.; Fagliari,J.J.
The efficiency of the Polymerase Chain Reaction (PCR) combined with selective enrichment broth was compared with the standard microbiological techniques for detection of Salmonella Dublin in fecal samples of 10 to 15-days-old Holstein calves, experimentally infected with 10(8) CFU of Salmonella Dublin. Seventy-six fecal samples were analyzed using PCR associated with selenite cystine (SC) and Muller-Kauffmann tetrathionate (TMK) broths and standard microbiological techniques. Regardless of the selective enrichment broth used, the standard microbiological techniques were significantly better than PCR in detection of positive samples of Salmonella Dublin. However, the simultaneous use of both techniques provided detection of a larger number of positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calves; Feces; Salmonella Dublin; PCR; Culture.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000300035

Fate of non O157 Shigatoxigenic Escherichia coli in ovine manure composting
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Marin,J.M.; Maluta,R.P.; Borges,C.A.; Beraldo,L.G.; Maesta,S.A.; Lemos,M.V.F.; Ruiz,U.S.; Ávila,F.A.; Rigobelo,E.C.
Livestock manure may contain pathogenic microorganisms which pose a risk to the health of animal or humans if the manure is not adequately treated or disposed of. To determine the fate of Shiga toxigenic Escherichia coli (STEC) non O157 in composted manure from naturally colonized sheep, fresh manure was obtained from animals carrying bacterial cells with stx1/ stx2 genes. Two composting systems were used, aerated and non-aerated, and the experiments were done in Dracena city, São Paulo State. Every week, for seven weeks, one manure sample from six different points in both systems was collected and cultured to determine the presence of E. coli, the presence of the virulence genes in the cells, and also the susceptibility to 10 antimicrobial drugs. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterial elimination; VTEC; Environment; Compost; Virulence gene.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000601771

Identification of new isolates of Bacillus thuringiensis using rep-PCR products and delta-endotoxin electron microscopy
Provedor de dados: Genetics and Molecular Biology
Autores: Lima,A.S.G.; Guidelli,A.M.; Abreu,I.L.; Lemos,M.V.F.
PCR has been used to analyze the distribution of REP (Repetitive Extragenic Palindromic) and ERIC (Enterobacterial Repetitive Intergenic Consensus) sequences (rep-PCR) found within the genome of the bacterium Bacillus thuringiensis, with the purpose to analyze the genetic similarities among 56 subspecies samples and 95 field isolates. The PCR products were analyzed by EB-AGE (ethidium bromide-agarose electrophoresis) and then submitted to banding comparisons, based on the Phyllip software algorithm. When the banding similarities were considered for comparison purposes among all the strains, the phylogenic tree patterns varied according to the rep-PCR primers considered, but, from a broader point of view, the ERIC sequences produced better results, which,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: REP; ERIC; Crystal protein; Parasporal bodies.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200017

The contribution of genes required for anaerobic respiration to the virulence of Salmonella enterica serovar Gallinarum for chickens
Provedor de dados: Brazilian Journal of Microbiology
Autores: Paiva,J.B.; Penha Filho,R.A.C.; Pereira,E.A.; Lemos,M.V.F.; Barrow,P.A.; Lovell,M.A.; Berchieri Jr,A.
Salmonella enterica serovar Gallinarum (SG) is an intracellular pathogen of chickens. To survive, to invade and to multiply in the intestinal tract and intracellularly it depends on its ability to produce energy in anaerobic conditions. The fumarate reductase (frdABCD), dimethyl sulfoxide (DMSO)-trimethylamine N-oxide (TMAO) reductase (dmsABC), and nitrate reductase (narGHIJ) operons in Salmonella Typhimurium (STM) encode enzymes involved in anaerobic respiration to the electron acceptors fumarate, DMSO, TMAO, and nitrate, respectively. They are regulated in response to nitrate and oxygen availability and changes in cell growth rate. In this study mortality rates of chickens challenged with mutants of Salmonella Gallinarum, which were defective in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella Gallinarum; Anaerobic genes; Poultry; Mutations.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400035