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Antioxidant activities of kombucha prepared from three different substrates and changes in content of probiotics during storage Ciênc. Tecnol. Aliment.
Fu,Caili; Yan,Fen; Cao,Zeli; Xie,Fanying; Lin,Juan.
Kombucha is a health-promoting fermented beverage worldwide. The present study compared the free-radical scavenging abilities and total reducing power (TRP) of kombucha prepared from low-cost green tea (LGTK), black tea (BTK), and tea powder (TPK). LGTK had the highest scavenging abilities against 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide anion and hydroxyl radicals, while BTK showed the highest TRP. Changes in content of probiotics in LGTK were investigated during storage as well. The number of acetic acid bacteria decreased moderately up to 10 days of storage. The number of lactic acid bacteria (LAB) decreased significantly, and their survival rate was only 0.98% at the 8th day of storage.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Kombucha; Antioxidant activities; Probiotics; Storage.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612014000100018
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Isolation and molecular characterization of a cax gene from Capsella bursa-pastoris Biocell
Lin,Juan; Zhang,Wen; Shi,Mingzhu; Wang,Xinglong; Sun,Xiaofen; Tang,Kexuan.
A new cation exchangers (CAXs) gene was cloned and characterized from Capsella bursapastoris by rapid amplification of cDNA ends (RACE). The full-length cDNA sequence of cax from C. bursa-pastoris (designated as Cbcax51) was 1754 bp containing a 1398 bp open reading frame encoding a polypeptide of 466 amino-acid residues with a calculated molecular mass of 50.5 kDa and an isoelectric point of 5.69. The predicted CbCAX51 contained an IMP dehydrogenase/GMP reductase domain, two Na+/Ca2+ exchanger protein domains. Comparative and bioinformatics analyses revealed that CbCAX51 showed extensive homology with CAX from other plant species. The expression analysis by different treatments indicated that Cbcax51 could be activated by cold triggering and was related...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Capsella bursa-pastoris; Cation exchangers (CAXs); RACE; Freezing tolerance.
Ano: 2008 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000300003
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Genomic cloning and characterization of a PPA gene encoding a mannose-binding lectin from Pinellia pedatisecta Biocell
Lin,Juan; Zhou,Xuanwei; Fei,Jiong; Liao,Zhihua; Jin,Wang; Sun,Xiaofen; Tang,Kexuan.
A gene encoding a mannose-binding lectin, Pinellia pedatisecta agglutinin (PPA), was isolated from leaves of Pinellia pedatisecta using genomic walker technology. The ppa contained an 1140-bp 5’-upstream region, a 771-bp open reading frame (ORF) and an 829-bp 3’-downstream region. The ORF encoded a precursor polypeptide of 256 amino acid residues with a 24-amino acid signal peptide. There were one putative TATA box and six possible CAAT boxes lying in the 5’-upstream region of ppa. The ppa showed significant similarity at the nucleic acid level with genes encoding mannose-binding lectins from other Araceae species such as Pinellia ternata, Arisaema heterophyllum, Colocasia esculenta and Arum maculatum. At the amino acid level, PPA also shared varying...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genomic cloning; Genomic walker technology; Mannose-binding lectin; Pinellia pedatisecta; Pinellia pedatisecta agglutinin (PPA).
Ano: 2006 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000100003
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Examination of camptothecin and 10-hydroxycamptothecin in Camptotheca acuminata plant and cell culture, and the affected yields under several cell culture treatments Biocell
Pi,Yan; Jiang,Keji; Hou,Rong; Gong,Yifu; Lin,Juan; Sun,Xiaofen; Tang,Kexuan.
Camptothecin and its derivatives are monoterpenoid indole alkaloids exhibiting significant anti-tumor actions. With the aim of improving the production of these pharmaceuticals, the contents of camptothecin and 10-hydroxycamptothecin in different tissues including roots, stems, leaves, young flower buds, opening flowers, fading flowers and seeds from Camptotheca acuminata, were investigated. The young flower buds had the highest alkaloid concentrations (camptothecin, 2.46 mg/g of dry weight; 10-hydroxycamptothecin, 1.41 mg/g of dry weight). Callus showed lower concentrations but it should also be considered as a potential source of these pharmaceuticals. In the present study, the growth rate of Camptotheca acuminata cells in culture did not correlate with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: HPLC; Salicylic acid; UV-B.
Ano: 2010 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000300006
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