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c-Fos expression induced by electroacupuncture at the Zusanli point in rats submitted to repeated immobilization BJMBR
Medeiros,M.A.; Canteras,N.S.; Suchecki,D.; Mello,L.E.A.M..
In laboratory animals, acupuncture needs to be performed on either anesthetized or, if unanesthetized, restrained subjects. Both procedures up-regulate c-Fos expression in several areas of the central nervous system, representing therefore a major pitfall for the assessment of c-Fos expression induced by electroacupuncture. Thus, in order to reduce the effect of acute restraint we used a protocol of repeated restraint for the assessment of the brain areas activated by electroacupuncture in adult male Wistar rats weighing 180-230 g. Repeated immobilization protocols (6 days, 1 h/day and 13 days, 2 h/day) were used to reduce the effect of acute immobilization stress on the c-Fos expression induced by electroacupuncture at the Zusanli point (EA36S). Animals...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acupuncture; Zusanli point; Immediate early genes; C-Fos; Restraint; Habituation.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200009
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Chronic excitotoxic lesion of the dorsal raphe nucleus induces sodium appetite BJMBR
Cavalcante-Lima,H.R.; Badauê-Passos Jr.,D.; de-Lucca Jr.,W.; Lima,H.R.C.; Costa-e-Sousa,R.H.; Olivares,E.L.; Cedraz-Mercez,P.L.; Reis,R.O.; Medeiros,M.A.; Côrtes,W.S.; Reis,L.C..
We determined if the dorsal raphe nucleus (DRN) exerts tonic control of basal and stimulated sodium and water intake. Male Wistar rats weighing 300-350 g were microinjected with phosphate buffer (PB-DRN, N = 11) or 1 µg/0.2 µl, in a single dose, ibotenic acid (IBO-DRN, N = 9 to 10) through a guide cannula into the DRN and were observed for 21 days in order to measure basal sodium appetite and water intake and in the following situations: furosemide-induced sodium depletion (20 mg/kg, sc, 24 h before the experiment) and a low dose of dietary captopril (1 mg/g chow). From the 6th day after ibotenic acid injection IBO-DRN rats showed an increase in sodium appetite (12.0 ± 2.3 to 22.3 ± 4.6 ml 0.3 M NaCl intake) whereas PB-DRN did not exceed 2 ml (P <...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sodium appetite; Water intake; Dorsal raphe nucleus; Serotonergic system; Ibotenic acid.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100015
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High-frequency electroacupuncture versus carprofen in an incisional pain model in rats BJMBR
Teixeira,F.M.; Castro,L.L.; Ferreira,R.T.; Pires,P.A.; Vanderlinde,F.A.; Medeiros,M.A..
The objective of the present study was to compare the effect of electroacupuncture (EA) and carprofen (CP) on postoperative incisional pain using the plantar incision (PI) model in rats. A 1-cm longitudinal incision was made through skin, fascia and muscles of a hind paw of male Wistar rats and the development of mechanical and thermal hypersensitivity was determined over 4 days using the von Frey and Hargreaves methods, respectively. Based on the experimental treatments received on the third postoperative day, the animals were divided into the following groups: PI+CP (CP, 2 mg/kg, po); PI+EAST36 (100-Hz EA applied bilaterally at the Zusanli point (ST36)); PI+EANP (EA applied to a non-acupoint region); PI+IMMO (immobilization only); PI (vehicle). In the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hyperalgesia; Plantar incision; Acupuncture; Zusanli point; Non-steroidal anti-inflammatory.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200014
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