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	Provedor de dados Rev. Bras. Ciênc. Avic. (4) Autor Montassier,HJ (4) Berchieri Jr,A (1) Berchieri Júnior, (1) Brentano,L (1) Fernandes,AC (1) Furuyama,CRAG (1) Givisiez,PEN (1) Montassier,MFSM (1) Okino,CH (1) Oliveira,GH (1) Oliveira,GH de<A NAME="nt01a"></A> (1) Mais... Palavra-chave Chicken (2) ELISA (2) Salmonella sp (2) Serology (2) Chickens (1) Conjugate (1) Coronavirus (1) Detection (1) Differentiation (1) Enzyme (1) Genetic evolution (1) Infectious bronchitis virus (1) Mutation (1) Prophylaxis (1) Recombination (1) Semi-nested RT-PCR (1) Vaccine strain (1) Variant strains (1) Mais... Tipo do documento Info:eu-repo/semantics/article (4) Ano 2010 (1) 2006 (1) 2005 (1) 2004 (1) País Brazil (4) Idioma Inglês (4)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 4 Primeira ... 1 ... Última Molecular epidemiology and evolution of avian infectious bronchitis virus Rev. Bras. Ciênc. Avic. Montassier,HJ. Mutation and recombination processes are involved in the genetic and phenotypic variations of RNA viruses, leading to the emergence of new variant strains, and give rise to virus population diversity to be modeled by the host, particularly by the immune system, as occurred with infectious bronchitis virus (IBV) in chickens. The consequence is a continuous emergence of new IBV variants with regard to pathotypes, serotypes, and protectotypes. Nucleotide sequencing and subsequent genetic analysis of the S1 and N protein gene sequences provide a fast and accurate method to classify and predict IBV genotype, and a powerful instrument to monitor phylogenetic and epidemiological evolution of IBV variants. Despite the use of vaccination programmes, infectious... Tipo: Info:eu-repo/semantics/article Palavras-chave: Chicken; Coronavirus; Genetic evolution; Mutation; Prophylaxis; Recombination; Variant strains. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2010000200003 Infectious bronchitis virus: detection and vaccine Strain differentiation by semi-nested RT-PCR Rev. Bras. Ciênc. Avic. Okino,CH; Montassier,MFSM; Givisiez,PEN; Furuyama,CRAG; Brentano,L; Montassier,HJ. A semi-nested reverse transcription-polymerase chain reaction (Semi-N-RT-PCR) was developed and used to detect the S glycoprotein gene of infectious bronchitis virus (IBV) strains and to discriminate H120 vaccine strain from other strains. Viral RNA was extracted from the allantoic fluid of chicken embryos and from tissues of chickens experimentally infected with different strains of IBV. Amplification and identification of the viral RNA was performed using two sets of primers complementary to a region of the S glycoprotein gene in the Semi-N-RT-PCR assay. The pair of primers used in the first PCR consisted of universal oligonucleotides flanking a more variable region of S1-S2 gene. The second primer pair was used in the Semi-N-RT-PCR and was comprised of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Detection; Differentiation; Infectious bronchitis virus; Semi-nested RT-PCR; Vaccine strain. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2005000100010 Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa Rev. Bras. Ciênc. Avic. Oliveira,GH; Berchieri Jr,A; Montassier,HJ. This study evaluated two enzyme-linked immunosorbent assays (ELISA) in the detection of chicken serologic response against Salmonella enterica sorotype Typhimurium. The assays have used as detecting antigen the soluble bacterial proteins of a non-flagellated strain of Salmonella Typhimurium (AgTM), and antibody conjugated to peroxidase or alkaline phosphatase. According to the results, optimal dilutions of antigen (concentration 5.49 mg/mL) and serum samples in both assays were 1:20,000 and 1:1,000, respectively. In such conditions, the ELISA/AgTM was able to detect serological response to Salmonella Typhimurium. Cross-reactions to Salmonella serotypes Gallinarum and Pullorum were seen, but not with other serotypes such as Enteritidis. Tipo: Info:eu-repo/semantics/article Palavras-chave: Chicken; ELISA; Enzyme; Salmonella sp; Serology. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000100008 Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa Rev. Bras. Ciênc. Avic. Oliveira,GH de<A NAME="nt01a"></A>; Berchieri Júnior,Â; Montassier,HJ; Fernandes,AC. This study was done to assess an enzyme-linked immunosorbent assay (ELISA) to detect chicken serological response against Salmonella enterica serotypes Gallinarum and Pullorum. The assays have used soluble bacterial proteins of Salmonella Gallinarum strain 9 (AgSG) as detecting antigen and peroxidase and alkaline phosphatase conjugates. According to the results, the antigen, sera and conjugate concentrations were optimized. In addition, the assay using alkaline phosphatase and peroxidase conjugates was helpful to distinguish positive serological reaction to serotypes Gallinarum and Pullorum from Enteritidis. Tipo: Info:eu-repo/semantics/article Palavras-chave: Chickens; Conjugate; ELISA; Salmonella sp; Serology. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000200007 Registros recuperados: 4 Primeira ... 1 ... Última

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