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Transcriptomic Profiling of Egg Quality in Sea Bass (Dicentrarchus labrax) Sheds Light on Genes Involved in Ubiquitination and Translation ArchiMer
Zarski, Daniel; Thaovi Nguyen,; Le Cam, Aurelie; Montfort, Jerome; Dutto, Gilbert; Vidal, Marie-odile; Fauvel, Christian; Bobe, Julien.
Variable and low egg quality is a major limiting factor for the development of efficient aquaculture production. This stems from limited knowledge on the mechanisms underlying egg quality in cultured fish. Molecular analyses, such as transcriptomic studies, are valuable tools to identify the most important processes modulating egg quality. However, very few studies have been devoted to this aspect so far. Within this study, the microarray-based transcriptomic analysis of eggs (of different quality) of sea bass (Dicentrarchus labrax) was performed. An Agilent oligo microarray experiment was performed on labelled mRNA extracted from 16 batches of eggs (each batch obtained from a different female) of sea bass, in which over 24,000 published probe arrays were...
Tipo: Text Palavras-chave: Microarray; Genomics; Transcriptomics; Aquaculture; Controlled reproduction.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00371/48220/48345.pdf
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A unique in vivo experimental approach reveals metabolic adaptation of the probiotic Propionibacterium freudenreichii to the colon environment ArchiMer
Saraoui, Taous; Parayre, Sandrine; Guernec, Gregory; Loux, Valentin; Montfort, Jerome; Le Cam, Aurelie; Boudry, Gaelle; Jan, G; Falentin, Helene.
Background Propionibacterium freudenreichii is a food grade bacterium consumed both in cheeses and in probiotic preparations. Its promising probiotic potential, relying largely on the active release of beneficial metabolites within the gut as well as the expression of key surface proteins involved in immunomodulation, deserves to be explored more deeply. Adaptation to the colon environment is requisite for the active release of propionibacterial beneficial metabolites and constitutes a bottleneck for metabolic activity in vivo. Mechanisms allowing P. freudenreichii to adapt to digestive stresses have been only studied in vitro so far. Our aim was therefore to study P. freudenreichii metabolic adaptation to intra-colonic conditions in situ. Results We...
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Ano: 2013 URL: http://archimer.ifremer.fr/doc/00171/28205/26433.pdf
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