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Comparative Study of Three Different ELISA to Measure the Antibodies Against Infectious Bronchitis Virus in Vaccinated and Unvaccinated Broilers 89
Cardoso,TC; Oliveira,C; Da-Silva,SEL; Ferreira,HL; Pinto,AA.
Broilers were spray-vaccinated (n=150) with H120 serotype at one-day-old, challenged after 28 days with M41 IBV serotype and after bled at day 28, 34 and 46 after challenged. The respective sera were tested by the indirect ELISA (I-ELISA), sandwich ELISA (S-ELISA), liquid phase blocking ELISA (LPB-ELISA) and the standard serum neutralization test (SNT). For this purpose, a total of 300 sera samples, 150 from non vaccinated and 150 from vaccinated broilers were titrated by all the serological methods and the correlation coefficients were determined. The correlation values (r) between LPB-ELISA x SNT, S-ELISA x SNT, I-ELISA x SNT found werer = 0.98, r = 0.79, and r = 0.74, respectively. Nevertheless, the r between LPB-ELISA x S-ELISA, LPB-ELISA x I-ELISA and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: ELISA; Post vaccinal response; Infectious bronchitis virus; Broilers.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2001000100010
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Serosurvey of selected avian pathogens in brazilian commercial Rheas (Rhea americana) and Ostriches (Struthio camelus) 89
Freitas Neto,OC de; Carrasco,AOT; Raso,TF; Sousa,RLM; Berchieri Júnior,A; Pinto,AA.
Ratite farming of has expanded worldwide. Due to the intensive farming methods used by ratite producers, preventive medicine practices should be established. In this context, the surveillance and control of some avian pathogens are essential for the success of the ratite industry; however, little is known on the health status of ratites in Brazil. Therefore, the prevalence of antibodies against Newcastle Disease virus, Chlamydophila psittaci, Mycoplasma gallisepticum, Mycoplasma synoviae, and Salmonella Pullorum were evaluated in 100 serum samples collected from commercial ostriches and in 80 serum samples from commercial rheas reared in Brazil. All sampled animals were clinically healthy. The results showed that all ostriches and rheas were serologically...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chlamydophila psittaci; Mycoplasma spp.; Newcastle Disease virus; Ostrich; Rhea; Salmonella spp.; Serology.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2009000400010
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Presence of antibodies against H5, H7 and H9 influenza A virus in wild birds in the State of São Paulo, Brazil 89
Sousa,E de; Costa,T P; Werther,K; Durigon,EL; Araujo,J de; Ferreira,CS; Pinto,AA.
Although the natural reservoirs of the avian influenza (AI) virus have been extensively studied in many countries, there is a clear lack of information on this subject in South America, particularly in Brazil. The objective of this study was to conduct a serological survey for H5, H7 and H9 antibodies to AI-subtype viruses in wild birds in the state of São Paulo , Brazil. Serum samples were tested using the hemagglutination-inhibition assay. Out of the 31 wild birds sampled between January and December of 2006, seven (22.58%), were seropositive for H5, H7 and H9; four (12.90%) were seropositive for H5 and H7; 13 (41.94%), were seropositive only for H7; three (9.7%), were seropositive only for H9; and four (12.90%) were negative for all three hemagglutinin...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Avian influenza virus; Brazil; Hemagglutination inhibition; Reservoir; Wild bird.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2013000300001
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