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Optimization of Bacillus subtilis cell growth effecting jiean-peptide production in fed batch fermentation using central composite design Electron. J. Biotechnol.
Zhong,Juan; Zhang,Xiaoyong; Ren,Yanli; Yang,Jie; Tan,Hong; Zhou,Jinyan.
Background Optimization of nutrient feeding was developed to improve the growth of Bacillus subtilis in fed batch fermentation to increase the production of jiean-peptide (JAA). A central composite design (CCD) was used to obtain a model describing the relationship between glucose, total nitrogen, and the maximum cell dry weight in the culture broth with fed batch fermentation in a 5 L fermentor. Results The results were analyzed using response surface methodology (RSM), and the optimized values of glucose and total nitrogen concentration were 30.70 g/L and 1.68 g/L in the culture, respectively. The highest cell dry weight was improved to 77.50 g/L in fed batch fermentation, which is 280% higher than the batch fermentation concentration (20.37 g/L). This...
Tipo: Journal article Palavras-chave: Bacillus subtilis ZK8; Iturin A; Response surface methodology.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000300005
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