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Registros recuperados: 6
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Isolation of 12 microsatellite markers following a pyrosequencing procedure and cross-priming in two invasive cryptic species, Alexandrium catenella (group IV) and A. tamarense (group III) (Dinophyceae) 5
Laporte, Martin; Shao, Zhaojun; Berrebi, Patrick; Laabir, Mohamed; Abadie, Eric; Faivre, Nicolas; Rieuvilleneuve, Fabien; Masseret, Estelle.
Alexandrium catenella (group IV) and Alexandrium tamarense (group III) (Dinophyceae) are two cryptic invasive phytoplankton species belonging to the A. tamarense species complex. Their worldwide spread is favored by the human activities, transportation and climate change. In order to describe their diversity in the Mediterranean Sea and understand their settlements and maintenances in this area, new microsatellite markers were developed based on Thau lagoon (France) samples of A. catenella and A. tamarense strains. In this study twelve new microsatellite markers are proposed. Five of these microsatellite markers show amplifications on A. tamarense and ten on A. catenella. Three of these 12 microsatellite markers allowed amplifications on both cryptic...
Tipo: Text Palavras-chave: Invasive species; Phytoplankton; Alexandrium; Dinophyceae; Microsatellite markers; Biogeography.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00189/30026/29398.pdf
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Exceptional but vulnerable microbial diversity in coral reef animal surface microbiomes 5
Chiarello, Marlène; Auguet, Jean-christophe; Graham, Nicholas A. J.; Claverie, Thomas; Sucré, Elliott; Bouvier, Corinne; Rieuvilleneuve, Fabien; Restrepo-ortiz, Claudia Ximena; Bettarel, Yvan; Villéger, Sébastien; Bouvier, Thierry.
Coral reefs host hundreds of thousands of animal species that are increasingly threatened by anthropogenic disturbances. These animals host microbial communities at their surface, playing crucial roles for their fitness. However, the diversity of such microbiomes is mostly described in a few coral species and still poorly defined in other invertebrates and vertebrates. Given the diversity of animal microbiomes, and the diversity of host species inhabiting coral reefs, the contribution of such microbiomes to the total microbial diversity of coral reefs could be important, yet potentially vulnerable to the loss of animal species. Analysis of the surface microbiome from 74 taxa, including teleost fishes, hard and soft corals, crustaceans, echinoderms,...
Tipo: Text Palavras-chave: Skin microbiota; Phylogenetic diversity; Conservation; Marine biodiversity; Octocorallia; Scleratinia.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00629/74121/73596.pdf
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Paralytic Toxins Accumulation and Tissue Expression of alpha-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate Alexandrium catenella 5
Rolland, Jean-luc; Pelletier, Kevin; Masseret, Estelle; Rieuvilleneuve, Fabien; Savar, Veronique; Santini, Adrien; Amzil, Zouher.
The pacific oyster Crassostrea gigas was experimentally exposed to the neurotoxic Alexandrium catenella and a non-producer of PSTs, Alexandrium tamarense (control algae), at concentrations corresponding to those observed during the blooming period. At fixed time intervals, from 0 to 48 h, we determined the clearance rate, the total filtered cells, the composition of the fecal ribbons, the profile of the PSP toxins and the variation of the expression of two a-amylase and triacylglecerol lipase precursor (TLP) genes through semi-quantitative RT-PCR. The results showed a significant decrease of the clearance rate of C. gigas fed with both Alexandrium species. However, from 29 to 48 h, the clearance rate and cell filtration activity increased only in oysters...
Tipo: Text Palavras-chave: Crassostrea gigas; Alexandrium catenella; PSP toxins; Digestion; Gene expression.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00130/24170/22172.pdf
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Skin microbiome of coral reef fish is highly variable and driven by host phylogeny and diet 5
Chiarello, Marlene; Auguet, Jean-christophe; Bettarel, Yvan; Bouvier, Corinne; Claverie, Thomas; Graham, Nicholas A. J.; Rieuvilleneuve, Fabien; Sucre, Elliot; Bouvier, Thierry; Villeger, Sebastien.
Background: The surface of marine animals is covered by abundant and diversified microbial communities, which have major roles for the health of their host While such microbiomes have been deeply examined in marine invertebrates such as corals and sponges, the microbiomes living on marine vertebrates have received less attention. Specifically, the diversity of these microbiomes, their variability among species, and their drivers are still mostly unknown, especially among the fish species living on coral reefs that contribute to key ecosystem services while they are increasingly affected by human activities. Here, we investigated these knowledge gaps analyzing the skin microbiome of 138 fish individuals belonging to 44 coral reef fish species living in the...
Tipo: Text Palavras-chave: Tropical; Teleost; Microbiota; Phylogenetic diversity; Phylosymbiosis; Phylogenetic signal.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00454/56565/74934.pdf
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Algicidal effects of Zostera marina L. and Zostera noltii Hornem. extracts on the neuro-toxic bloom-forming dinoflagellate Alexandrium catenella 5
Laabir, Mohamed; Grignon-dubois, Micheline; Masseret, Estelle; Rezzonico, Bernadette; Soteras, Giselle; Rouquette, Manuel; Rieuvilleneuve, Fabien; Cecchi, Philippe.
The inhibitory effects of crude extracts of Zostera marina L and Zostera noltii Hornemann on the growth of the toxic red tide dinoflagellate Alexandrium catenella were investigated through bio-assays. Methanolic and aqueous extracts were prepared from fresh and detrital leaves of Z. marina and Z. noltii collected in the Thau lagoon and Arcachon bay (France). All the extracts exhibited significant inhibition of A. catenella growth, whatever the species and without the need of continuous addition of extracts. The effective concentrations (EC50) varied in the range 0.036-0.199 g L-1 for Z. noltii and 0.036-0.239 g L-1 for Z. marina. Methanolic extracts prepared from fresh plant tissues were the most potent, with EC50 of 0.036-0.039 and 0.036-0.045 g L-1, for...
Tipo: Text Palavras-chave: Growth inhibition; Allelopathy; Alexandrium catenella; Zostera marina; Zostera noltii; Phenolics.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00170/28095/26720.pdf
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Unsuspected intraspecific variability in the toxin production, growth and morphology of the dinoflagellate Alexandrium pacificum R.W. Litaker (Group IV) blooming in a South Western Mediterranean marine ecosystem, Annaba Bay (Algeria) 5
Hadjadji, Imen; Laabir, Mohamed; Frihi, Hocine; Collos, Yves; Shao, Zhao Jun; Berrebi, Patrick; Abadie, Eric; Amzil, Zouher; Chomérat, Nicolas; Rolland, Jean-luc; Rieuvilleneuve, Fabien; Masseret, Estelle.
Physiological plasticity gives HABs species the ability to respond to variations in the surrounding environment. The aim of this study was to examine morphological and physiological variability in Alexandrium pacificum R.W. Litaker (Group IV) (former Alexandrium catenella) blooming in Annaba bay, Algeria. Monoclonal cultures of up to 30 strains of this neurotoxic dinoflagellate were established by the germination of single resting cysts from the surface sediment of this southern Mediterranean marine ecosystem. Ribotyping confirmed formally for the first time that A. pacificum is developing in Eastern Algerian waters. Toxin analyses of A. pacificum strains revealed substantial intraspecific variability in both the profile and toxin amount. However, the...
Tipo: Text Palavras-chave: Alexandrium pacificum; Mediterranean; Toxin profile; Growth; Intraspecific variability.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00624/73620/73598.pdf
Registros recuperados: 6
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