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Genetic variability and efficiency of DNA microsatellite markers for paternity testing in horse breeds from the Brazilian Marajó archipelago Genet. Mol. Biol.
Reis,Sávio P.; Gonçalves,Evonnildo C.; Silva,Artur; Schneider,Maria P.C..
In this study, 15 microsatellite DNA loci used in comparative tests by the International Society for Animal Genetics were applied to the evaluation of genetic diversity and management, and the efficiency of paternity testing in Marajoara horses and Puruca ponies from the Marajó Archipelago. Based on the genotyping of 93 animals, mean allelic diversity was estimated as 9.14 and 7.00 for the Marajoara and Puruca breeds, respectively. While these values are similar to those recorded in most European breeds, mean levels of heterozygosity were much lower (Marajoara 49%, Puruca 40%), probably as a result of high levels of inbreeding in the Marajó populations. The mean informative polymorphic content of this 15-marker system was over 50% in both breeds, and was...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Marajoara horses; Puruca ponies; Microsatellite markers; Genetic diversity; Geneaological control.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100014
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Exposure to an extremely low-frequency electromagnetic field only slightly modifies the proteome of Chromobacterium violaceum ATCC 12472 Genet. Mol. Biol.
Baraúna,Rafael A.; Santos,Agenor V.; Graças,Diego A.; Santos,Daniel M.; Ghilardi Júnior,Rubens; Pimenta,Adriano M. C.; Carepo,Marta S. P.; Schneider,Maria P.C.; Silva,Artur.
Several studies of the physiological responses of different organisms exposed to extremely low-frequency electromagnetic fields (ELF-EMF) have been described. In this work, we report the minimal effects of in situ exposure to ELF-EMF on the global protein expression of Chromobacterium violaceum using a gel-based proteomic approach. The protein expression profile was only slightly altered, with five differentially expressed proteins detected in the exposed cultures; two of these proteins (DNA-binding stress protein, Dps, and alcohol dehydrogenase) were identified by MS/MS. The enhanced expression of Dps possibly helped to prevent physical damage to DNA. Although small, the changes in protein expression observed here were probably beneficial in helping the...
Tipo: Info:eu-repo/semantics/report Palavras-chave: C. violaceum; Electromagnetic field; Proteomic analysis.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200227
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