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GPo1 alkB gene expression for improvement of the degradation of diesel oil by a bacterial consortium BJM
Luo,Qun; He,Ying; Hou,Deng-Yong; Zhang,Jian-Guo; Shen,Xian-Rong.
To facilitate the biodegradation of diesel oil, an oil biodegradation bacterial consortium was constructed. The alkane hydroxylase (alkB) gene of Pseudomonas putida GPo1 was constructed in a pCom8 expression vector, and the pCom8-GPo1 alkB plasmid was transformed into Escherichia coli DH5α. The AlkB protein was expressed by diesel oil induction and detected through SDS-polyacrylamide gel electrophoresis. The culture of the recombinant (pCom8-GPo1 alkB/E. coli DH5α) with the oil biodegradation bacterial consortium increased the degradation ratio of diesel oil at 24 h from 31% to 50%, and the facilitation rates were increased as the proportion of pCom8-GPo1 alkB/E. coli DH5α to the consortium increased. The results suggested that the expression of the GPo1...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alkane hydroxylase; Expression; Escherichia coli DH5α; Biodegradation; Diesel oil.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300649
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