Data/hora: 27/07/2024 03:10:03

Provedor de dados: 	Arquivo Brasileiro de Medicina Veterinária e Zootecnia	
País: 	Brazil	
Título: 	Detection of Mycoplasma hyopneumoniae in lungs and nasal swabs of pigs by nested PCR	
Autores: 	Silva,F.M.F.; Castro,L.A.; Silva Júnior,A.; Moraes,M.P.; Moreira,M.A.S.; Almeida,M.R.	
Data: 	2009-02-01	
Ano: 	2009	
Palavras-chave: 	Swine; Enzootic pneumonia; Mycoplasma hyopneumoniae; Nested PCR.	
Resumo: 	Fifty-four samples were collected from growing and finishing pigs for the molecular diagnosis of enzootic porcine pneumonia. Nineteen lung fragments were obtained from pigs that showed signs of respiratory disease and 35 nasal swabs were obtained from clinically healthy pigs. For the detection of the bacterial genome in the samples, the nested PCR technique was used to amplify a fragment of 706bp. This fragment was subsequently cloned and sequenced. The sequence of obtained nucleotides was compared with six other sequences of Mycoplasma hyopneumoniae and 11 sequences of other bacteria available in the Genbank. To measure the sensitivity of the nested PCR, serial dilutions (10-1 to 10-15) of cloned fragments were conducted based on the concentration of 300ng. Ten lung fragments and eight nasal swabs showed positive for M. hyopneumoniae and the limit of detection was estimated to be 0.3fg DNA cloned. The sequence of nucleotides obtained showed 99.1% homology with the other sequences of M. hyopneumoniae, demonstrating that the nested PCR used in this study may provide an important diagnostic tool for the detection of this agent.	
Tipo: 	Info:eu-repo/semantics/report	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000100021	
Editor: 	Universidade Federal de Minas Gerais, Escola de Veterinária	
Relação: 	10.1590/S0102-09352009000100021	
Formato: 	text/html	
Fonte: 	Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.61 n.1 2009	
Fonte: 	info:eu-repo/semantics/openAccess