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Prediction of mutations on structure primase of the archaeon Sulfolobus solfataricus Biological Sciences
Souza, Eden Silva e; Diniz, Michely Correia.
 All living organisms need a DNA replication mechanism and it has been conserved in the three domains of life throughout evolutionary process. Primase is the enzyme responsible for synthesizing de novo RNA primers in DNA replication. Archaeo-Eukaryotic Primase (AEP) is the superfamily that typically forms a heterodimeric complex containing both a small catalytic subunit (PriS) and a large accessory noncatalytic subunit (PriL). Sulfolobus solfataricus is a model organism for research on the Genetics field. The aim of this work was to evaluate, via Bioinformatics tools, three mutations in the large subunit (PriL) of the archaeon Sulfolobus solfataricus. The aspartic acid residue in the positions (Asp) 62, (Asp) 235, (Asp) 241 have been substituted by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2.00.00.00-6; 2.02.02.00-8 in silico; PriL; 3D structure; DNA replication; Primer; Mutations. Genética Molecular e de Microorganismos.
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34600
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Genetic diversity of 16S rRNA and mcrA genes from methanogenic archaeas Biological Sciences
Xavier, Keyla Vitoria Marques; Souza, Eden Silva e; Santos, Erick de Aquino; Diniz, Michely Correia.
Methanogenic archaeas are found in aquatic and terrestrial environments and are fundamental in the conversion of organic matter into methane, a gas that has a potential use as renewable source of energy, which is also considered as one of the main agents of the greenhouse effect. The vast majority of microbial genomes can be identified by a conservative molecular marker, the 16S ribosomal gene. However, the mcrA gene have been using in studies of methanogenic archaea diversity as an alternative marker, highly conserved and present only in methanogens. This gene allows the expression of the enzyme Methyl-coenzyme M reductase, the main agent in converting by-products of anaerobic digestion into methane. In this context, we aimed to study the genetic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Heterozygosity; Microbiomes; Methane; Anaerobic; Arlerquin; Databases heterozygosity; Microbiomes; Methane; Anaerobic; Arlerquin; Databases..
Ano: 2020 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/49877
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