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Group III PLA2 from the scorpion, Mesobuthus tamulus: cloning and recombinant expression in E. coli Electron. J. Biotechnol.
Hariprasad,Gururao; Saravanan,Kolandaivelu; Singh,Sundararajan Baskar; Das,Utpal; Sharma,Sujata; Kaur,Punit; Singh,Tej Pal; Srinivasan,Alagiri.
Phospholipases A2 (PLA2) are enzymes that specifically hydrolyze the sn-2 fatty acid acyl bond of phospholipids, producing a free fatty acid and a lyso-phospholipid. We report the cloning and expression of a secretory phospholipase A2 (sPLA2) from Mesobuthus tamulus, Indian red scorpion. The nucleotide sequence codes for a 167 residue enzyme. The open reading frame codes for a 31 amino acid signal peptide followed by a mature portion of the protein. The primary structure shows the calcium binding motif, catalytic residues, 8 highly-conserved cysteines and C-terminal extension which classify it as a group III PLA2. The entire transcript was expressed in Escherichia coli and was purified by metal affinity chromatography under denaturing conditions. The...
Tipo: Journal article Palavras-chave: Group III phospholipase A2; Mesobuthus tamulus; Recombinant expression.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300006
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