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	Provedor de dados Biocell (4) Autor Sun,Xiaofen (4) Tang,Kexuan (4) Lin,Juan (3) Wang,Xinglong (2) Deng,Zhongxiang (1) Fei,Jiong (1) Gong,Yifu (1) Hou,Rong (1) Jiang,Keji (1) Jin,Wang (1) Liao,Zhihua (1) Liu,Xiaojun (1) Pi,Yan (1) Shi,Mingzhu (1) Yongzhen,Pang (1) Zhang,Wen (1) Zhonghai,Chen (1) Zhou,Xuanwei (1) Mais... Palavra-chave Mannose-binding lectin (2) RACE (2) Capsella bursa-pastoris (1) Cation exchangers (CAXs) (1) Freezing tolerance (1) Genomic cloning (1) Genomic walker technology (1) HPLC (1) Pinellia pedatisecta (1) Pinellia pedatisecta agglutinin (PPA) (1) Salicylic acid (1) UV-B (1) ZAA (1) Zantedeschia aethiopica (1) Mais... Tipo do documento Info:eu-repo/semantics/article (4) Ano 2010 (1) 2008 (1) 2006 (1) 2005 (1) País Argentina (4) Idioma Inglês (4)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 4 Primeira ... 1 ... Última Isolation and molecular characterization of a cax gene from Capsella bursa-pastoris Biocell Lin,Juan; Zhang,Wen; Shi,Mingzhu; Wang,Xinglong; Sun,Xiaofen; Tang,Kexuan. A new cation exchangers (CAXs) gene was cloned and characterized from Capsella bursapastoris by rapid amplification of cDNA ends (RACE). The full-length cDNA sequence of cax from C. bursa-pastoris (designated as Cbcax51) was 1754 bp containing a 1398 bp open reading frame encoding a polypeptide of 466 amino-acid residues with a calculated molecular mass of 50.5 kDa and an isoelectric point of 5.69. The predicted CbCAX51 contained an IMP dehydrogenase/GMP reductase domain, two Na+/Ca2+ exchanger protein domains. Comparative and bioinformatics analyses revealed that CbCAX51 showed extensive homology with CAX from other plant species. The expression analysis by different treatments indicated that Cbcax51 could be activated by cold triggering and was related... Tipo: Info:eu-repo/semantics/article Palavras-chave: Capsella bursa-pastoris; Cation exchangers (CAXs); RACE; Freezing tolerance. Ano: 2008 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000300003 Molecular cloning and characterization of a novel mannosebinding lectin cDNA from Zantedeschia aethiopica Biocell Zhonghai,Chen; Yongzhen,Pang; Liu,Xiaojun; Wang,Xinglong; Deng,Zhongxiang; Sun,Xiaofen; Tang,Kexuan. Using RNA extracted from Zantedeschia aethiopica young leaves and primers designed according to the conservative regions of Araceae lectins, the full-length cDNA of Z. aethiopica agglutinin (zaa) was cloned by rapid amplification of cDNA ends (RACE). The full-length cDNA of zaa was 871 bp and contained a 417 bp open reading frame (ORF) encoding a lectin precursor of 138 amino acids. Through comparative analysis of zaa gene and its deduced amino acid sequence with those of other Araceae species, it was found that zaa encoded a precursor lectin with signal peptide. Secondary and three-dimensional structure analyses showed that ZAA had many common characters of mannose-binding lectin superfamily and ZAAwas a mannose-binding lectin with three mannose-binding... Tipo: Info:eu-repo/semantics/article Palavras-chave: Mannose-binding lectin; RACE; Zantedeschia aethiopica; ZAA. Ano: 2005 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000200006 Genomic cloning and characterization of a PPA gene encoding a mannose-binding lectin from Pinellia pedatisecta Biocell Lin,Juan; Zhou,Xuanwei; Fei,Jiong; Liao,Zhihua; Jin,Wang; Sun,Xiaofen; Tang,Kexuan. A gene encoding a mannose-binding lectin, Pinellia pedatisecta agglutinin (PPA), was isolated from leaves of Pinellia pedatisecta using genomic walker technology. The ppa contained an 1140-bp 5’-upstream region, a 771-bp open reading frame (ORF) and an 829-bp 3’-downstream region. The ORF encoded a precursor polypeptide of 256 amino acid residues with a 24-amino acid signal peptide. There were one putative TATA box and six possible CAAT boxes lying in the 5’-upstream region of ppa. The ppa showed significant similarity at the nucleic acid level with genes encoding mannose-binding lectins from other Araceae species such as Pinellia ternata, Arisaema heterophyllum, Colocasia esculenta and Arum maculatum. At the amino acid level, PPA also shared varying... Tipo: Info:eu-repo/semantics/article Palavras-chave: Genomic cloning; Genomic walker technology; Mannose-binding lectin; Pinellia pedatisecta; Pinellia pedatisecta agglutinin (PPA). Ano: 2006 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000100003 Examination of camptothecin and 10-hydroxycamptothecin in Camptotheca acuminata plant and cell culture, and the affected yields under several cell culture treatments Biocell Pi,Yan; Jiang,Keji; Hou,Rong; Gong,Yifu; Lin,Juan; Sun,Xiaofen; Tang,Kexuan. Camptothecin and its derivatives are monoterpenoid indole alkaloids exhibiting significant anti-tumor actions. With the aim of improving the production of these pharmaceuticals, the contents of camptothecin and 10-hydroxycamptothecin in different tissues including roots, stems, leaves, young flower buds, opening flowers, fading flowers and seeds from Camptotheca acuminata, were investigated. The young flower buds had the highest alkaloid concentrations (camptothecin, 2.46 mg/g of dry weight; 10-hydroxycamptothecin, 1.41 mg/g of dry weight). Callus showed lower concentrations but it should also be considered as a potential source of these pharmaceuticals. In the present study, the growth rate of Camptotheca acuminata cells in culture did not correlate with... Tipo: Info:eu-repo/semantics/article Palavras-chave: HPLC; Salicylic acid; UV-B. Ano: 2010 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000300006 Registros recuperados: 4 Primeira ... 1 ... Última

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