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Registros recuperados: 6
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Development of microsatellite markers for Hoplias malabaricus (Erythrinidae). Repositório Alice
GONDIM, S. G. C. A.; RESENDE, L. V.; BRONDANI, R. P. V.; COLLEVATTI, R. G.; SILVA-JÚNIOR, N. J.; PEREIRA, R. R.; TELLES, M. P. C..
We identified 14 microsatellite loci for the wolf fish, Hoplias malabaricus (Erythrinidae), from a genomic shotgun library. Twenty-five primers were designed, and 48 individuals of H. malabaricus from four localities of northwest Goiás, in central Brazil, were genotyped to characterize the polymorphism at each locus. Fourteen primers amplified clearly interpretable products using a single PCR protocol; six loci were polymorphic, but with a low number of alleles per locus (2 or 3). Expected heterozygosities for polymorphic loci ranged from 0.136 to 0.505. Combined paternity exclusion probability (0.638) was low and combined genetic identity (0.056) was high in studies of parentage. The low polymorphism may be due to the small microsatellite size and the...
Tipo: Artigo de periódico Palavras-chave: Microssatélite; Cerrado; Traira; Hoplias malabaricus; Marcador molecular.
Ano: 2010 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/862333
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Estrutura genética espacial em população natural de Annona crassiflora Mart. (Annonaceae) do cerrado. Repositório Alice
GOUVEIA, F. O.; COLLEVATTI, R. G.; TELLES, M. P. C.; BORBA, T. C. O.; SOARES, T. N..
O objetivo desse estudo foi estimar o nível de diversidade genética e estrutura genética espacial dentro de uma população de A. crassiflora.
Tipo: Parte de livro Palavras-chave: Diversidade genética; Araticum; Annona crassiflora; Fruta tropical; Genética; Cerrado.
Ano: 2010 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/857912
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Paternity testing and behavioral ecology: a case study of jaguars (Panthera onca) in Emas National Park, Central Brazil. Repositório Alice
SOARES, T. N.; TELLES, M. P. C.; RESENDE, L. V.; SILVEIRA, L.; JÁCOMO, A. T. A.; MORATO, R. G.; DINIZ-FILHO, J. A. F.; EIZIRIK, E.; BRONDANI, R. P. V.; BRONDANI, C..
We used microsatellite loci to test the paternity of two male jaguars involved in an infanticide event recorded during a long-term monitoring program of this species. Seven microsatellite primers originally developed for domestic cats and previously selected for Panthera oncawere used. In order to deal with uncertainty in the mother?s genotypes for some of the loci, 10000 values of Wwere derived by simulation procedures. The male that killed the two cubs was assigned as the true sire. Although the reasons for this behavior remain obscure, it shows, in principle, a low recognition of paternity and kinship in the species. Since the two cubs were not very young, one possibility is that the adult male did not recognize the cubs and killed them for simple...
Tipo: Artigo de periódico Palavras-chave: Ecological stress; Infanticide; Jaguar; Microsatellites.; Paternity..
Ano: 2006 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/214576
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Development of microsatellite markers for the endangered neotropical tree species Tibouchina papyrus (Melastomataceae). Repositório Alice
TELLES, M. P. C.; PEIXOTO, F. P.; LIMA, J. S.; RESENDE, L. V.; VIANELLO, R. P.; WALTER, M. E. M. T.; COLLEVATTI, R. G..
We isolated and characterized 12 microsatellite loci for Tibouchina papyrus (Melastomataceae), an endangered species with narrow and disjunct range, endemics to a few localities in ?cerrado rupestre? from Central Brazil. These microsatellites were obtained by sequencing of a genomic shotgun library for primer design. Leaves from 96 individuals collected in the three known local populations were genotyped using the 12 primers designed to analyze the polymorphisms at each locus. The number of alleles per locus ranged from one to six; two loci were monomorphic. Among the polymorphic loci, expected heterozygosities ranged from 0.161 to 0.714. Combined paternity exclusion probability was 0.957 and combined genetic identity (0.051) was high for studies on...
Tipo: Artigo de periódico Palavras-chave: Tibouchina papyrus; Pau-papel; Cerrado; Árvore.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/882313
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Isolation and characterization of microsatellite markers in the armored catfish Hypostomus gymnorhynchus (Loricariidae). Repositório Alice
TELLES, M. P. C.; RESENDE, L. V.; BRONDANI, R. P. V.; COLLEVATTI, R. G.; COSTA, M. C.; SILVA JÚNIOR, N. J..
We isolated and characterized 10 microsatellite loci in the armored catfish (Hypostomus gymnorhynchus, Loricariidae), using a genomic shotgun library to obtain the repetitive sequences. Twenty-four primers were designed and 14 individuals of H. gymnorhynchus from the Caiapó River, in central Brazil, were genotyped using these primers to analyze the polymorphism at each locus. All loci showed low polymorphism, with a low number of alleles per locus (1 or 2), except locus Hg_E19, which had 11 alleles. Expected heterozygosities for polymorphic loci ranged from 0.182 to 0.901. Combined paternity exclusion probability (0.857) was low and combined genetic identity (0.0026) was high, when we examined parentage. The low degree of polymorphism that we detected may...
Tipo: Artigo de periódico Palavras-chave: Hypostomus gymnorhynchus; Microsatélite; Cerrado; Catfish.
Ano: 2010 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/862340
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Development and characterization of new microsatellites for Eugenia dysenterica DC (Myrtaceae). Repositório Alice
TELLES, M. P. C.; SILVA, J. B.; RESENDE, L. V.; VIANELLO, R. P.; CHAVES, L. J.; SOARES, T. N.; COLLEVATTI, R. G..
Microsatellite markers were developed for population genetic analyses of the Neotropical tree Eugenia dysenterica DC (Myrtaceae), after construction of a shotgun genomic library for microsatellite discovery. Nine primers were designed, of which 5 yielded amplified product. These primers were polymorphic for 97 individuals collected in 3 distinct localities. The number of alleles per locus (primer) ranged from 3 to 11 and expected heterozygosities varied from 0.309 to 0.884. The probability of locus identity was ~1.88 x 10-4 and the probability of paternity exclusion was ~0.9367. The 5 microsatellite primer pairs may be suitable for population genetic studies such as parentage and fine-scale genetic analyses of this species.
Tipo: Artigo de periódico Palavras-chave: Fruta tropical; Cerrado; Cagaita; Marcador molecular; Variação genética; Genetic variation; Microsatellite repeats.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/978390
Registros recuperados: 6
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