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	Provedor de dados Trop. Plant Pathol. (1) Genet. Mol. Biol. (1) Autor Travensolo,Regiane F. (2) Carareto-Alves,Lucia M. (1) Carrilho,Emanuel (1) Ciapina,Luciane P. (1) Costa,Maria V.C.G. (1) Lemos,Eliana G. M. (1) Lemos,Eliana G.M. (1) Lopes,Tiago J.S. (1) Mais... Palavra-chave DNA microarray (1) Gene expression (1) Oligonucleotide (1) PCR (1) Polymerase chain reaction (1) REP-PCR (1) Xylella fastidiosa (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2009 (1) 2005 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Development of a scar marker for Pierce's Disease strains of Xylella fastidiosa Trop. Plant Pathol. Travensolo,Regiane F.; Ciapina,Luciane P.; Lemos,Eliana G. M.. The objective of this research was to develop a primer for a polymerase chain reaction specific for Xylella fastidiosa strains that cause Pierce's Disease (PD) in grapes (Vitis vinifera). The DNA amplification of 23 different strains of X. fastidiosa, using a set of primers REP1-R (5'-IIIICGICGIATCCIGGC-3') and REP 2 (5'-ICGICTTATCIGGCCTAC-3') using the following program: 94 ºC/2 min; 35 X (94 ºC/1 min, 45 ºC/1 min and 72 ºC/1 min and 30 s) 72 ºC/5 min, produced a fragment of 630 bp that differentiated the strains that cause disease in grapes from the other strains. However, REP banding patterns could not be considered reliable for detection because the REP1-R and REP 2 primers correspond to repetitive sequences, which are found throughout the bacterial... Tipo: Info:eu-repo/semantics/article Palavras-chave: Oligonucleotide; Polymerase chain reaction; PCR; REP-PCR. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000200002 Xylella fastidiosa gene expression analysis by DNA microarrays Genet. Mol. Biol. Travensolo,Regiane F.; Carareto-Alves,Lucia M.; Costa,Maria V.C.G.; Lopes,Tiago J.S.; Carrilho,Emanuel; Lemos,Eliana G.M.. Xylella fastidiosa genome sequencing has generated valuable data by identifying genes acting either on metabolic pathways or in associated pathogenicity and virulence. Based on available information on these genes, new strategies for studying their expression patterns, such as microarray technology, were employed. A total of 2,600 primer pairs were synthesized and then used to generate fragments using the PCR technique. The arrays were hybridized against cDNAs labeled during reverse transcription reactions and which were obtained from bacteria grown under two different conditions (liquid XDM2 and liquid BCYE). All data were statistically analyzed to verify which genes were differentially expressed. In addition to exploring conditions for X. fastidiosa... Tipo: Info:eu-repo/semantics/article Palavras-chave: Xylella fastidiosa; DNA microarray; Gene expression. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200022 Registros recuperados: 2 Primeira ... 1 ... Última

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