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Monitoring human cytomegalovirus viral load in peripheral blood leukocytes of renal transplant recipients by a simple limiting dilution-PCR assay
Provedor de dados: 56
Autores: Caballero,O.L.; Costa,M.C.S.L.; Trevisan,A.; Oliveira,R.M.; Viotti,E.A.; Távora,E.R.F.; Vilaça,S.S.; Sabagga,E.; de-Paula,F.J.; Távora,P.F.; Brasileiro-Filho,G.; Villa,L.L.; Simpson,A.J.G.
To assess the clinical relevance of a semi-quantitative measurement of human cytomegalovirus (HCMV) DNA in renal transplant recipients within the typical clinical context of a developing country where virtually 100% of both receptors and donors are seropositive for this virus, we have undertaken HCMV DNA quantification using a simple, semi-quantitative, limiting dilution polymerase chain reaction (PCR). We evaluated this assay prospectively in 52 renal transplant patients from whom a total of 495 serial blood samples were collected. The samples scored HCMV positive by qualitative PCR had the levels of HCMV DNA determined by end-point dilution-PCR. All patients were HCMV DNA positive during the monitoring period and a diagnosis of symptomatic infection was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymerase chain reaction; Human cytomegalovirus; Renal transplant; Diagnosis; Viral load.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200010