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Searching for the best real-time RT-PCRs to detect Zika virus infections: the importance of comparing several protocols BJMBR
de Moraes,F.M.; Espósito,D.L.A.; Klein,T.M.; da Fonseca,B.A.L..
Clinical manifestations of Zika, dengue, and chikungunya virus infections are very similar, making it difficult to reach a diagnosis based only on clinical grounds. In addition, there is an intense cross-reactivity between antibodies directed to Zika virus and other flaviviruses, and an accurate Zika diagnosis is best achieved by real-time RT-PCR. However, some real-time RT-PCR show better performance than others. To reach the best possible Zika diagnosis, the analytic sensitivity of some probe-based real-time RT-PCR amplifying Zika virus RNA was evaluated in spiked and clinical samples. We evaluated primers and probes to detect Zika virus, which had been published before, and tested sensitivity using serum spiked and patient samples by real-time RT-PCR....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zika virus; Flavivirus; Real time RT-PCR; Diagnosis.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600618
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