Consulta: subjectFacets:"Autopsy"
Registros recuperados: 4
Data/hora: 27/07/2024 02:14:10

DNA extraction and quantification from touch and scrape preparations obtained from autopsy liver cells
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Ribeiro,C.N.M.; Peres,L.C.; Pina-Neto,J.M.
The objective of the present study was to develop a simplified low cost method for the collection and fixation of pediatric autopsy cells and to determine the quantitative and qualitative adequacy of extracted DNA. Touch and scrape preparations of pediatric liver cells were obtained from 15 cadavers at autopsy and fixed in 95% ethanol or 3:1 methanol:acetic acid. Material prepared by each fixation procedure was submitted to DNA extraction with the Wizard® genomic DNA purification kit for DNA quantification and five of the preparations were amplified by multiplex PCR (azoospermia factor genes). The amount of DNA extracted varied from 20 to 8,640 µg, with significant differences between fixation methods. Scrape preparation fixed in 95% ethanol provided...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autopsy; Congenital anomalies; DNA; Touch preparative; Scrape preparative; Polymerase chain reaction.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500002

Fatal disseminated mucormycosis in a patient with mantle cell non-hodgkin's lymphoma: an autopsy case
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Alacacioglu,Inci; Kargi,Aydanur; Ozcan,Mehmet Ali; Piskin,Ozden; Solak,Cilem; Secil,Mustafa; Unlu,Mehtat; Demirkan,Fatih; Ozsan,Guner Hayri; Undar,Bulent.
A patient with mantle cell non-Hodgkin's lymphoma presented herself with fever, nausea, right upper quadrant pain on the 7th day of R-CHOP chemotherapy. After hospitalization with the suspicion of acute cholecystitis, she received antibiotherapy with G-CSF because of emerging neutropenia at the 10th day of chemotherapy. Abdominal computed tomography revealed small infarcts in the spleen and kidneys. The echymotic lesion which developed on her right lateral malleolus, became bullous in the following days and treated as ecthyma gangrenosum. Altough the patient was afebrile with a normal neutrophil count on the third day of antibiotherapy, she developed acute renal failure and deteriorated rapidly. The patient underwent hemodialysis but expired on the 10th...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Mucormycosis; Lymphoma; Autopsy.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000300017

Record of postmortem injuries caused by the Neotropical social wasp Agelaia fulvofasciata (Degeer) (Hymenoptera, Vespidae) on pig carcasses in the Eastern Amazon region: implications in forensic taphonomy
Provedor de dados: Revista Brasileira de Entomologia
Autores: Barbosa,Rodrigo R.; Carriço,Cesar; Souto,Raimundo N. P.; Andena,Sergio R.; Ururahy-Rodrigues,Alexandre; Queiroz,Margareth M. C.
ABSTRACTPostmortem injuries are a source of misinterpretations in forensic analysis and therefore are subject matter of taphonomic interest. Many types of injuries can cause different artifacts, which deserve attention of the forensic pathologists when evaluating corpses, either at the crime scene or during an autopsy. Insects can be important biotaphonomic agents and their activity may result in artifacts that resemble antemortem injuries. Here, we describe postmortem injuries caused by the Neotropical wasp Agelaia fulvofasciata (Degeer, 1773) on domestic pig carcasses weighting 15 kg. The specimens showed extensive injuries to the lower lip, similar to lacerations, and some minor lesions on the snout and anus. In addition, we observed the same wasp...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Autopsy; Forensic Entomology; Savanna.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262015000300257

Risk factors associated with the death of patients hospitalized for juvenile systemic lupus erythematosus
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Faco,M.M.M.; Leone,C.; Campos,L.M.A.; Febrônio,M.V.; Marques,H.H.S.; Silva,C.A.
We assessed the risk factors associated with death in patients hospitalized for juvenile systemic lupus erythematosus (JSLE) and evaluated the autopsy reports. A total of 57,159 hospitalizations occurred in our institution from 1994 to 2003, 169 of them involving 71 patients with JSLE. The most recent hospitalization of these patients was evaluated. Patients were divided into two groups based on mortality during hospitalization: those who survived (N = 53) and those who died (N = 18). The main causes of hospitalization were JSLE activity associated with infection in 52% and isolated JSLE activity in 44%. Univariate analysis showed that a greater risk of death was due to severe sepsis (OR = 17.8, CI = 4.5-70.9), systemic lupus erythematosus disease activity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Juvenile systemic lupus erythematosus; Hospitalization; Outcome; Autopsy; Severe sepsis; Infection.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000700015