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Complementation of the Lactococcus lactis secretion machinery with Bacillus subtilis SecDF improves secretion of staphylococcal nuclease Inra
Nouaille, S.; Morello, E.; Cortes Perez, N.; Le Loir, Y.; Commissaire, J.; Gratadoux, J.J.; Poumerol, E.; Gruss, A.; Langella, P..
Unlike Bacillus subtilis and Escherichia coli, the gram-positive lactic acid bacterium Lactococcus lactis does notpossess the SecDF protein, a component of the secretion (Sec) machinery involved in late secretion stages andrequired for the high-capacity protein secretion in B. subtilis. In this study, we complemented the L. lactis Secmachinery with SecDF from B. subtilis and evaluated the effect on the secretion of two forms of staphylococcalnuclease, NucB and NucT, which are efficiently and poorly secreted, respectively. The B. subtilis SecDFencodinggene was tested in L. lactis at different levels. Increased quantities of the precursor and matureforms were observed only at low levels of SecDF and at high NucT production levels. This SecDF...
Tipo: Journal Article Palavras-chave: LACTOCOCCUS LACTIS; BACILLUS SUBTILIS; COMPLEMENTATION; SECRETION.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007bf702808&uri=/notices/prodinra1/2008/02/
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Biological control of Botrytis cinerea on stem wounds with moderately halophilic bacteria Inra
Sadfi-Zouaoui, N.; Hannachi, I.; Andurand, D.; Essghaier, B.; Boudabous, A.; Nicot, P..
Infection of tomato stem wounds by Botrytis cinerea is an important problem which can cause severe economic losses in greenhouse tomato crops. Three moderately halophilic bacteria were tested for their ability to protect pruning wounds from attacks by B. cinerea under growth chamber conditions. The severity of the disease estimated by the length of the rotted stem was used to calculate the area under the disease progress curves (AUDPC). Bacterial antagonists (B1, B2 and B3) were very effective in controlling Botrytis-infection on the tomato stems during the first 6 days and later by the end of the experiment. Plants treated with Bacillus subtilis (B1) had the lowest AUDPC (0). It was followed by B. subtilis (B3) and Halomonas sp. (B2) with AUDPC of 9.8 and...
Tipo: Journal Article Palavras-chave: BIOLOGICAL CONTROL; GREY MOULD; BACILLUS SUBTILIS; HALOMONAS SP.; TOMATO PLANTS; SPORE GERMINATION; BOTRYTIS CINEREA.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008a057db88&uri=/notices/prodinra1/2009/06/
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