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Biological and molecular characterization of Streptococcus crista strains isolated from human dental biofilm by means of arbitrary primers - PCR (AP-PCR) BJM
Angelini,Michelle; Silveira,Wanderley Dias da; Campos,Tatiana Amabile de; Cai,Silvana; Castro,Antonio Fernando Pestana de.
Streptococcus ssp are important components of the dental biofilm and Streptococcus crista is considered to be an interesting model of bacterial interactions taking place in this biofilm. In the present work, S. crista strains were isolated from the dental biofilm of Brazilian individuals and studied with respect to their biological characteristics and their molecular profile by means of AP-PCR techniques, using the RR2, 434, OPR2, OPR8, and OPR13 primers. Results allowed us to build a similarity dendrogram. Analysis of the similarity dendrogram allowed the separation of the studied strains into similarity groups. All isolates presented fibril tufts by Transmission Electron Microscopy (TEM). These isolates were able to bind to salivary amylase and to adhere...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological characterization; Molecular characterization; Dendrogram analysis; Biofilm.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200005
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Prevalence of newcastle disease virus in broiler chickens (Gallus gallus) in Brazil BJM
Orsi,M.A; Doretto Jr,L; Camillo,S.C.A; Reischak,D; Ribeiro,S.A.M; Ramazzoti,A; Mendonça,A.O; Spilki,F.R; Buzinaro,M.G; Ferreira,H.L; Arns,C.W.
This study was carried out during 2002/2003, aiming to determine the prevalence of virulent Newcastle disease virus strains (NDV) in Brazilian commercial poultry farms. Clinical samples were obtained from the Southeastern, Southern and Central-Western regions, which comprise the main area of the Brazilian poultry production. Serum samples and tracheal and cloacal swabs of 23,745 broiler chickens from 1,583 flocks, including both vaccinated chickens and those with no vaccination information, were tested for NDV using a diagnostic ELISA kit. The seropositivity was 39.1%, and the isolation percentage by flock varied from 1.0 to 7.6%, and by region from 6.5 to 58.4%. Higher isolation rates (74.3-83.3%) were obtained after three passages in embryonated chicken...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Newcastle Disease Virus; NDV-free status; Pathogenicity; Broiler chickens; Biological characterization.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200014
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A survey for maintenance of virulent newcastle disease virus-free area in poultry production in Brazil BJM
Orsi,M.A; Doretto Jr,L; Camillo,S.C.A; Reischak,D; Ribeiro,S.A.M; Ramazzoti,A; Mendonça,A.O; Spilki,F.R; Buzinaro,M.G; Ferreira,H.L; Arns,C.W.
In 2003, Brazil was recognized as a pathogenic Newcastle Disease Virus (NDV) strain-free country for commercial poultry. This research was conducted in Brazil between December 2003 and March 2005 to verify the maintenance of this virulent NDV-free status. Serum samples from 5,455 flocks for commercial poultry farms were collected, comprising 81,825 broiler chickens. The farms were located in nine states of the country, grouped in three geographic regions. Serological evidence of NDV infection was detected in 28.8% of the surveyed farms. However, all fifteen viruses isolated and identified as Newcastle Disease Virus (NDV) were characterized as nonpathogenic strains, based on the Intracerebral Pathogenicity Index. These results showed that Brazil preserves...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Newcastle Disease Virus; Pathogenicity; Poultry; Biological characterization.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200017
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Produção de forragem de genotipos de leucaena spp em duas épocas do ano. Repositório Alice
PRIMAVESI, A. C. P. de A.; PRIMAVESI, O. M. A. S. P. R.; NOVAES, N. J..
Com a finalidade de verificar entre os genotipos testados, aqueles que se destacam quanto a produção de matéria seca, em relação a testemunha Cunningham.
Tipo: Separatas Palavras-chave: Leucaena spp; Avaliação de genotipos; Caracterização biologica; Evoluation of genotypes; Biological characterization.
Ano: 1994 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/42393
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Study of biological characteristics of Pseudomonas aeruginosa strains isolated from patients with cystic fibrosis and from patients with extra-pulmonary infections BJID
Stehling,Eliana Guedes; Silveira,Wanderley Dias da; Leite,Domingos da Silva.
A total of 120 strains of Pseudomonas aeruginosa, isolated from cystic fibrosis (CF) patients (n = 80) and from patients having extra-pulmonary infections (n = 40) were studied regarding the presence of some virulence factors (hemolysin, gelatinase and elastase production) and presence of the algD and algU genes as detected by polymerase chain reaction-PCR. There was not a significant difference for the production of gelatinase and hemolysin between non-mucoid strains from CF patients and other isolates from extra-pulmonary infections and mucoid strains. The production of elastase was found to be significant among these strains. The algD gene was detected by PCR in all studied strains but the algU gene was detected only in 25% of the mucoid strains....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Biological characterization; Pathogenesis; Cystic fibrosis; Extra-pulmonary infections.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000100018
Registros recuperados: 5
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