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Alternativas para a redução de custos na produção de mudas in vitro. Infoteca-e
RIBEIRO, J. M.; PINTO, M. dos S. T.; TEIXEIRA, S. L..
Este trabalho apresenta alternativas que podem contribuir para a redução de custos no processo de produção de mudas in vitro. Substituição de componentes do meio nutritivo, substituição da autoclavagem pela esterilização química do meio nutritivo e utilização de biorreatores de imersão temporária são algumas das alternativas abordadas na publicação. Trata-se de uma boa fonte informação para aqueles que desejam ampliar seus conhecimentos acerca do tema, que deve ser amplamente discutido, pois é aderente a um dos nossos maiores desafios: a produção de alimentos em complexos cenários climáticos
Tipo: Documentos (INFOTECA-E) Palavras-chave: Produção de mudas.; Biotecnologia; Cultura de Tecido; Micropropagação.; Biotechnology.
Ano: 2013 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/982095
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Amino acid features: a missing compartment of prediction of protein function Nature Precedings
Esmaeil Ebrahimie; Mansour Ebrahimi; Mahdi Ebrahimi.
Enormous computational efforts have been carried out to predict structure and function of protein. However, nearly all of these efforts have been focused on prediction of function based on primary nucleic acid sequence or modeling 3D structure of protein from its nucleic acid sequence. In fact, it seems that amino acid attributes, which is an intermediate phase between DNA/RNA and advanced protein structure, has been missed.
From 2010, we examined the possibility of precise prediction of structural protein function based on amino acid features by improving the following three aspects of amino acid research: (1) Increasing the number of computationally calculated amino acid features, (2) Testing different feature selection (attribute...
Tipo: Manuscript Palavras-chave: Biotechnology; Cancer; Bioinformatics.
Ano: 2011 URL: http://precedings.nature.com/documents/6693/version/1
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An albumin-derived peptide scaffold capable of binding and catalysis Nature Precedings
Immacolata Luisi; Silvia Pavan; Giampaolo Fontanive; Alessandro Tossi; Fabio Benedetti; Adriano Savoini; Elisa Maurizio; Riccardo Sgarra; Daniele Sblattero; Federico Berti.
We have identified a 101-amino-acid polypeptide derived from the sequence surrounding the IIA binding site of human albumin. The polypeptide contains residues that make contact with ligands as warfarin in the parent protein, and eight cysteine residues to form disulfide bridges, which stabilize the polypeptide structure. Seventy-four amino acids are located in six [alpha]-helical regions, with the remaining amino acids forming six connecting coil/loop regions. Codon usage optimization was used to express a GST fusion protein in E. coli in yields as high as 4 mg/l. This fusion protein retains its structural integrity and aldolase activity, the ability to direct the stereochemical outcome of a diketone reduction, and its binding capacity to warfarin and...
Tipo: Manuscript Palavras-chave: Biotechnology; Chemistry.
Ano: 2012 URL: http://precedings.nature.com/documents/6894/version/1
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An Analysis of McLean County, Illinois Farmers' Perceptions of Genetically Modified Crops AgEcon
Chimmiri, Nagesh; Tudor, Kerry W.; Spaulding, Aslihan D..
McLean County, Illinois farmers were surveyed in order to explore and analyze their perceptions of genetically modified crops and their genetically modified cropping decisions. Questionnaires were mailed to 400 randomly selected farmers, and 156 were returned. The 134 respondents who reported that they planned to plant crops in 2003 were asked to provide information about gender, age, education, and number of tillable acres farmed. Respondents were also asked if they had previously planted genetically modified crops and if they planned to plant either genetically modified corn or genetically modified soybeans in 2003. Finally, respondents were presented with 40 statements that pertained to biotechnology and genetically modified crops, and they were asked...
Tipo: Conference Paper or Presentation Palavras-chave: Biotechnology; Genetically modified; Corn; Soybeans; Research and Development/Tech Change/Emerging Technologies; Q16.
Ano: 2005 URL: http://purl.umn.edu/19204
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An Analysis of Pricing in the U.S. Cotton Seed Market AgEcon
Shi, Guanming; Stiegert, Kyle W.; Chavas, Jean-Paul.
The purpose of the research in this paper is to investigate the impact of differentiated vertical strategies by agricultural biotechnology firms in the U.S. cottonseed market. The model advances the measurement of industry concentration to consider substitution/complementarity relationships among differentiated products delivered under different vertical structures. We find evidence of sub-additive pricing in the stacking of bundled biotech traits. Prices paid by farmers for cottonseed sold under vertical integration are found to be higher than under licensing. The model is flexible and allows for evaluation of the effects of changing market structures. The parameters on traditional measures of concentration indicate that higher concentration leads to...
Tipo: Conference Paper or Presentation Palavras-chave: Modal Vertical Strategy; Imperfect competition; Cotton seed; Biotechnology; Agricultural and Food Policy; Demand and Price Analysis; Industrial Organization; L13; L4; L65.
Ano: 2009 URL: http://purl.umn.edu/51617
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An analysis of trade related international regulations of genetically modified food and their effects on developing countries AgEcon
Gruere, Guillaume P..
This paper reviews current trade-related regulations of genetically modified (GM) food and discusses their effects on developing countries. There is a large heterogeneity in current import approval and marketing policies of GM food worldwide. At the international level, the harmonization efforts are led by the Codex Alimentarius Commission, the Cartagena Protocol on Biosafety and the World Trade Organization. While internationally harmonized guidelines for safety approval have been finalized, we show that there is no clear consensus on labeling regulations for GM food, and there is an increasing risk of conflicts among international agreements. We analyze the GM food regulations of two large rich importers, Japan and the European Union (EU) and discuss...
Tipo: Report Palavras-chave: Genetically modified food; Developing countries; Biotechnology; Biosafety; Trade regulation; Labeling; International trade; Tariff; International Relations/Trade.
Ano: 2006 URL: http://purl.umn.edu/55422
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An approach able to escape the most common problem during protein Crystallization i.e. formation of salt crystal Nature Precedings
K. Awaneesh Singh; Patrick Celie; G. R. K. Rao; M. V. Jagannatham.
Formation and differentiation of salt crystal is a most common as well as challenging problem during protein crystallography. Although there are some methods used for their differentiation i.e. physical manipulation, birefringence, dye absorption and run a gel but all of them are totally depends on own perception and expertise as well as also require a good crystal (final stage crystal). To escape this problem the screening was done with PACT, JCSG and other automated screens as everyone do. But during optimization the additives/salts were ignored at first and optimization screen was made just based on different buffers and precipitant. After selecting suitable buffer, the type and percentage of precipitant was optimized. We got some ugly crystals and with...
Tipo: Poster Palavras-chave: Biotechnology; Chemistry.
Ano: 2010 URL: http://precedings.nature.com/documents/4725/version/1
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An attempt to understand Barstar, Barnase and Olfactory receptor protein folding problems using mathematical biological approach Nature Precedings
Arunava Goswami; Sk Sarif Hassan; Pabitra Pal Choudhury.
Protein folding problem as attracted structural biologists immensely (1). Till date, correlation between X-ray crystallographic and NMR data are considered to be the best methods for determining structure of intra-cellular proteins. Generating crystals and finding correct experimental conditions for NMR are largly a gamble and resultant data processing highly time consuming. Even if, a large number of laboratories around the world and India make crystals of protein but majority of them fail to generate crystallographic data less than or equal to 10A resolution with currently available instruments. This means that X-ray crystallographic data have been generated from protein crystals with non-uniform lattices. We believe that a large portion of (chain of...
Tipo: Manuscript Palavras-chave: Biotechnology; Genetics & Genomics; Bioinformatics; Evolutionary Biology.
Ano: 2010 URL: http://precedings.nature.com/documents/4891/version/1
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An Empirical Analysis of the Effects of Plant Variety Protection Legislation on Innovation and Transferability AgEcon
Srinivasan, Chittur S.; Shankar, Bhavani; Holloway, Garth J..
Under the TRIPs Agreement, all member-countries of the World Trade Organization are required to provide an "effective" system of plant variety protection within a specific time frame. In many developing countries this has led to a divisive debate about the fundamental desirability of extending intellectual property rights to agriculture. But empirical studies on the economic impacts of PVP, especially its ability to generate large private sector investments in plant breeding and facilitate the transfer of technology, have been very limited. This paper examines two aspects of the international experience of PVP legislation thus far (i) The relationship between legislation, R&D expenditures and PVP grants, i.e., the innovation effect, and (ii) The role...
Tipo: Conference Paper or Presentation Palavras-chave: Plant variety protection; Biotechnology; Technology transfer; Crop Production/Industries.
Ano: 2002 URL: http://purl.umn.edu/24788
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AN ERA OF CONFUSION: THE LAND GRANT RESEARCH AGENDA AND BIOTECHNOLOGY AgEcon
Weatherspoon, Dave D.; Oehmke, James F.; Raper, Kellie Curry.
The Land Grant College and University (LGCU) research system is in a state of confusion. Public research privatization, industry concentration, and balancing money and the Land Grant mission are issues LGCUs currently face. The ag-biotechnology revolution complicates these issues. This paper provides four mission related policy recommendations for the LGCU system. Keywords: Biotechnology, Land Grant Mission, Research
Tipo: Working or Discussion Paper Palavras-chave: Biotechnology; Land Grant Mission; Research; Research and Development/Tech Change/Emerging Technologies.
Ano: 2000 URL: http://purl.umn.edu/11559
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AN INITIAL LOOK AT THE TOKYO GRAIN EXCHANGE NON-GMO SOYBEAN CONTRACT AgEcon
Parcell, Joseph L..
Genetically modified organisms (GMOs) such as soybeans and corn have garnered considerable consumer attention due to the concern over potential effects from using these commodities as inputs into food production. In the simplest form, segregation of bioengineered and non-bioengineered crops is an identity-preserved system. For these identity-preserved systems to exist, there must be a market discovery mechanism in place whereby supply and demand factors interact to establish a market price. In May 2000, the Tokyo Grain Exchange began a nongenetically modified organism (non-GMO) soybean contract. This article describes information garnered from the first public-offered identity-preserved marketplace.
Tipo: Journal Article Palavras-chave: Biotechnology; Genetically modified organisms; Identity-preserved markets; Non-GMO; Soybean contract; Tokyo Grain Exchange; Marketing.
Ano: 2001 URL: http://purl.umn.edu/14684
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An innovative method for the biological control of Alternanthera philoxeroides Nature Precedings
Yusong Cao; Huiming Wang.
Biological invasion has become a serious global environmental problems and been considered as an important component of global change^1-2^. Invasions by alien species can have an impact at several levels of ecological complexity from genes to ecosystems^3-4^. Invasive alien species had seriously threated the biodiversity and natural ecosystems in China^5^, and caused economic losses by more than US$7 billion every year^6^. Alternanthera philoxeroides, commonly known as Alligator weed, is an invasive amphibious plant^7^. The effect of physical methods such as mechanical or artificial salvage, removal of A. philoxeroides were very limited. Moreover, these measure should be carried out very carefully or would easy lead to its rebound^8^. The mainly biological...
Tipo: Manuscript Palavras-chave: Biotechnology; Ecology; Plant Biology.
Ano: 2010 URL: http://precedings.nature.com/documents/5267/version/1
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An integrated solution for rapid biosensing combining linker free binding, freeze drying and high sensitivity ellipsometric detection Nature Precedings
Yongbai Yin; David R. McKenzie; Keith Fisher; Chuan Guo; Neil Nosworthy; Marcela M. Bilek.
A novel integrated biosensor methodology is proposed and demonstrated. The methodology utilizes a nitrogen-containing plasma polymer to achieve linker-free binding of biorecognition molecules that allows the sensor surface to be freeze dried for long shelf life under ambient conditions. The sensor is configured for single wavelength ellipsometric detection providing a low cost, versatile, and rapid sensing and diagnosis platform suitable for a wide range of applications and end-users. The merits of the methodology were demonstrated using three antigen-antibody pairs.
Tipo: Manuscript Palavras-chave: Biotechnology.
Ano: 2012 URL: http://precedings.nature.com/documents/6764/version/1
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An intelligent liposome that may deliver drug molecules in a well controlled fashion Nature Precedings
Dumitru Popescu; Alin Gabriel Popescu.
The passage of molecules, especially large ones, through the cellular membrane is a very important problem for some biotechnological applications, such as drug delivery. The appearance of pores in the lipid bilayer following some controlled mechanisms may be an adequate and interesting way. Some pores, named stochastic pores, can appear due to structural and dynamic properties of lipid bilayer, but others may be favored by mechanical tension induced by different ways. Recently, a sequence of 30-40 pores was observed in the same vesicle, a pore at a time, which can appear in vesicles stretched by optical induced mechanical tension. There are two very interesting biotechnological applications that require the increase of membrane permeability: gene therapy...
Tipo: Manuscript Palavras-chave: Biotechnology.
Ano: 2007 URL: http://precedings.nature.com/documents/1220/version/1
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An Olfactory Receptor Pseudogene whose Function emerged in Humans Nature Precedings
Peter Lai; Gautam Bahl; Maryse Gremigni; Valery Matarazzo; Olivier Clot-Faybesse; Catherine Ronin; Chiquito J. Crasto.
Human olfactory receptor, hOR17-210, is identified as a pseudogene in the human genome. Experimental data has shown however, that the gene product of cloned hOR17-210 cDNA was able to bind an odorant-binding protein and is narrowly tuned for excitation by cyclic ketones. Supported by experimental results, we used the bioinformatics methods of sequence analysis, computational protein modeling and docking, to show that functionality in this receptor is retained due to sequence-structure features not previously observed in mammalian ORs. This receptor does not possess the first two transmembrane helical domains (of seven typically seen in GPCRs). It however, possesses an additional TM that has not been observed in other human olfactory receptors. By...
Tipo: Manuscript Palavras-chave: Biotechnology; Neuroscience; Bioinformatics.
Ano: 2007 URL: http://precedings.nature.com/documents/1290/version/1
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Anais... Infoteca-e
SEMANA DA CAPRINOCULTURA E DA OVINOCULTURA TROPICAL BRASILEIRA, 1., 1994, Sobral..
Aspectos economicos da caprino-ovinocultura tropical brasileira; Caprinocultura leiteira no Brasil - estadio da arte e perspectivas; Estadio atual e perspectivas da ovinocultura tropical; Biotecnologia da reproducao caprina; Tecnologia e comercializacao de carne ovina; Industrializacao e comercializacao de pele; A modernidade cientifica e sua interfaces.
Tipo: Documentos (INFOTECA-E) Palavras-chave: Aspecto econômico; Congresso; Brasil; Ceara; Congress.; Caprinocultura; Ovinocultura; Comercialização; Mercado; Biotecnologia; Goats; Sheep; Commercialization; Biotechnology; Small ruminants; Brazil..
Ano: 1994 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/514313
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Anais: resumos dos trabalhos. Repositório Alice
ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENETICOS E BIOTECNOLOGIA, 5., 2000, Brasilia, DF..
Tipo: Anais e Proceedings de eventos Palavras-chave: Biotecnologia; Controle Biológico; Recurso Genético.; Genetic resources.; Biotechnology; Biological control.
Ano: 2000 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/179918
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Anais: resumos dos trabalhos. Repositório Alice
ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 7., 2002, Brasília, DF..
Tipo: Anais e Proceedings de eventos Palavras-chave: Recursos geneticos.; Biotecnologia; Controle Biológico.; Biological control; Biotechnology; Genetic resources..
Ano: 2002 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/184607
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Anais: resumos dos trabalhos. Repositório Alice
WORKSHOP DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 4., 1999, Brasília, DF..
Tipo: Anais e Proceedings de eventos Palavras-chave: Recursos geneticos.; Biotecnologia; Controle Biológico.; Biotechnology; Biological control; Genetic resources..
Ano: 1999 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/179268
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Anais: resumos dos trabalhos. Repositório Alice
ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 6., 2001, Brasília, DF..
Tipo: Anais e Proceedings de eventos Palavras-chave: Recursos geneticos.; Biotecnologia; Controle Biológico.; Biological control; Biotechnology; Genetic resources..
Ano: 2001 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/180288
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