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Applying of novel subtraction method Genetically Directed Differential Subtraction Chain (GDDSC) in plant genomes Nature Precedings
Ewa Siedlecka; Aneta Hromada-Judycka; Magdalena Pawełkowicz; Rafał Wóycicki; Monika Rakoczy-Trojanowska; Zbigniew Przybecki.
We present a simple subtraction procedure of GDDSC as a modification of the original DSC and GDRDA methods. Genetically Directed Differential Subtraction Chain (GDDSC) is a process by which highly related genomes are compared in order to isolate tags carrying the polymorphisms. 
To detect specific DNA fragment (tag), we can then monitor offspring plants for efficient molecular breeding. The GDDSC protocol was applied to isolation of new sex related clones from cucumber plants (_Cucumis_ _sativus_ L.) and new tissue culture response clones from rye (_Secale_ _cereale_ L.). The newly identified tags, obtained by GDDSC represent pools of candidate genes and other sequences, which could serve as potential markers for requested traits.
Tipo: Manuscript Palavras-chave: Biotechnology; Developmental Biology; Genetics & Genomics; Plant Biology.
Ano: 2011 URL: http://precedings.nature.com/documents/5465/version/2
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Aprimoramento de um processo enzimático para a síntese de ácido glicônico. Repositório Alice
SILVA, E. A. da; CUNHA, M. F.; OLIVEIRA, D. F. de; GAMBETTA, R.; PACHECO, T. F.; MENDES, T. D.; RODRIGUES, D. de S..
Atualmente, o ácido glicônico é produzido industrialmente pela oxidação microbiana de glicose. Entretanto, esse processo encontra‑se em vias de ser substituído pelo processo enzimático, o qual vem sendo fortemente investigado pela comunidade científica, visando melhorar os parâmetros de produção por meio, principalmente, de engenharia das enzimas e ajustes das condições operacionais do processo. O objetivo deste estudo foi aprimorar o processo de produção de ácido glicônico usando uma rota enzimática e glicose com elevado grau de pureza como substrato. A glicose oxidase de Aspergillus niger foi utilizada como enzima modelo. Foram avaliados os efeitos da concentração inicial de glicose, da presença de peroxidase e da aeração do meio reacional. A...
Tipo: Anais e Proceedings de eventos Palavras-chave: Ácido xilônico; Ácido glicônico; Glicose oxidase; Biotecnologia; Biotechnology.
Ano: 2020 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1127575
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Aptamer-based multiplexed proteomic technology for biomarker discovery Nature Precedings
Larry Gold; Deborah Ayers; Jennifer Bertino; Christopher Bock; Ashley Bock; Edward N. Brody; Jeff Carter; Virginia Cunningham; Andrew Dalby; Bruce E. Eaton; Tim Fitzwater; Dylan Flather; Ashley Forbes; Trudi Foreman; Cate Fowler; Bharat Gawande; Meredith Goss; Magda Gunn; Shashi Gupta; Dennis Halladay; Jim Heil; Joe Heilig; Brian Hicke; Gregory Husar; Nebojsa Janjic; Thale Jarvis; Susan Jennings; Evaldas Katilius; Tracy R. Keeney; Nancy Kim; Terese Kaske; Tad Koch; Stephan Kraemer; Luke Kroiss; Ngan Le; Daniel Levine; Wes Lindsey; Bridget Lollo; Wes Mayfield; Mike Mehan; Robert Mehler; Michele Nelson; Sally K. Nelson; Dan Nieuwlandt; Malti Nikrad; Urs Ochsner; Rachel M. Ostroff; Matt Otis; Thomas Parker; Steve Pietrasiewicz; Dan Resnicow; John Rohloff; Glenn Sanders; Sarah Sattin; Dan Schneider; Britta Singer; Martin Stanton; Alana Sterkel; Alex Stewart; Suzanne Stratford; Jonathan D. Vaught; Mike Vrkljan; Jeffrey J. Walker; Mike Watrobka; Sheela Waugh; Allison Weiss; Sheri Wilcox; Alexey Wolfson; Steve Wolk; Chi Zhang; Dom Zichi.
Interrogation of the human proteome in a highly multiplexed and efficient manner remains a coveted and challenging goal in biology. We present a new aptamer-based proteomic technology for biomarker discovery capable of simultaneously measuring thousands of proteins from small sample volumes (15 [mu]L of serum or plasma). Our current assay allows us to measure ~800 proteins with very low limits of detection (1 pM average), 7 logs of overall dynamic range, and 5% average coefficient of variation. This technology is enabled by a new generation of aptamers that contain chemically modified nucleotides, which greatly expand the physicochemical diversity of the large randomized nucleic acid libraries from which the aptamers are selected. Proteins in complex...
Tipo: Manuscript Palavras-chave: Biotechnology; Chemistry; Molecular Cell Biology; Bioinformatics.
Ano: 2010 URL: http://precedings.nature.com/documents/4538/version/1
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Aqueous synthesis of CdTe quantum dot as biological fluorescent probe for monitoring methyl parathion by fluoro-immunosensor Nature Precedings
Raghuraj Chouhan; Aaydha Vinayaka; Munna Thakur.
Bioconjugation of quantum dots (QDs) provide high resolution in biological fluorescent labelling as a result of physical and optical properties of QDs. This intrinsic property of QDs can be made use of for sensitive detection of target analytes including food and environmental monitoring. In this investigation, we report the bioconjugation of thiol stabilized CdTe QD for the sensitive detection of methyl parathion (MP) at picogram level. The specificity in the analysis was attributed by highly specific competitive immunological reactions between free MP and CdTe QD bioconjugated MP (MP-BSA-CdTe) for immobilized anti-MP IgY antibodies in a simple flow injection system. We also report the possible resonance energy transfer phenomenon as a result of...
Tipo: Manuscript Palavras-chave: Biotechnology.
Ano: 2009 URL: http://precedings.nature.com/documents/3451/version/1
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Arbuscular mycorrhizal fungus spores host bacteria and their biofilm efficient in nutrient biodynamics and soil-borne plant pathogen suppression Nature Precedings
Andre Cruz; Takaaki Ishii.
The combination of bacteria and arbuscular mycorrhizal fungi (AMF) would be ideal for nutrient biodvnamics in soils and protection from soil-borne plant pathogens (SBPP). During the process of plant colonization AMF interact with bacteria where spores cytoplasm and hvphae provide specific niches for certain populations of bacteria. The clarification of the relationship AMF-bacteria has great potential for sustainable agriculture. The currently research aimed to isolate and characterize bacteria. Which we have designated most probable endobacteria (MPE) from an AMF spore. Three bacterial strains were taken from about 500 spores by using a 200 µm diameter hvpodermic needle. These bacteria were identified by DNA and morphological methods. Antagonism...
Tipo: Manuscript Palavras-chave: Biotechnology; Microbiology.
Ano: 2011 URL: http://precedings.nature.com/documents/5544/version/1
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Archon Genomics X PRIZE Validation Protocol Nature Precedings
Larry Kedes; Granger Sutton; Edison Liu; Victor Jongeneel.
This document is a collective assembly of techniques designed to test the quality and accuracy of 100 whole human genome sequences resulting from the $10 Million Archon Genomics X PRIZE (AGXP) competition. The purpose of this article is to enlist constructive criticism from the genomic and genetic community on the outlined approaches. The intent for the final version of this Validation Protocol is to become a useful standard by which to gauge the capabilities of whole genome sequencing technologies that emerge even after 2012.
Tipo: Manuscript Palavras-chave: Biotechnology; Genetics & Genomics; Bioinformatics; Data Standards.
Ano: 2011 URL: http://precedings.nature.com/documents/5731/version/1
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Artificial Antigen Presenting Cells With Preclustered anti-CD28/-CD3/-LFA-1 Monoclonal Antibodies Are Highly Effective To Induce The Ex-Vivo Expansion Of Functional Human Antitumor T Cells Nature Precedings
Roberta Zappasodi; Massimo Di Nicola; Carmelo Carlo-Stella; Roberta Mortarini; Alessandra Molla; Claudia Vegetti; Lorena Passoni; Salvatore Albani; Andrea Anichini; Alessandro M. Gianni.
Effective adoptive T cell therapy requires the _ex vivo_ generation of functional T lymphocytes with a long lifespan _in vivo_. We evaluated _in vitro_ T cell expansion by artificial antigen presenting cells (aAPC) generated with activating (human anti-CD3), co-stimulating (human anti-CD28) and adhesion (human anti-LFA-1) monoclonal antibodies pre-clustered in microdomains (MDs) held by a liposome scaffold. The co-localization of T cell ligands in MDs and the targeting of an adhesion protein, increasing the efficiency of immunological synapse formations, represent the novelties of our system. These aAPCs allowed increased expansion of polyclonal CD4^+^ and CD8^+^ T cells and of tumor antigen-specific CD8^+^ T cells compared to anti-CD28- and...
Tipo: Manuscript Palavras-chave: Biotechnology; Cancer; Immunology.
Ano: 2007 URL: http://precedings.nature.com/documents/1250/version/1
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Artificial Photosynthesis Would Unify the Electricity-Carbohydrate-Hydrogen Cycle for Sustainability Nature Precedings
Yi-Heng Percival Zhang.
Sustainable development requires balanced integration of four basic human needs – air (O2/CO2), water, food, and energy. To solve key challenges, such as CO2 fixation, electricity storage, food production, transportation fuel production, water conservation or maintaining an ecosystem for space travel, we wish to suggest the electricity-carbohydrate-hydrogen (ECHo) cycle, where electricity is a universal energy carrier, hydrogen is a clean electricity carrier, and carbohydrate is a high-energy density hydrogen (14.8 H2 mass% or 11-14 MJ electricity output/kg)carrier plus a food and feed source. Each element of this cycle can be converted to the other reversibly & efficiently depending on resource availability, needs, and costs. In...
Tipo: Manuscript Palavras-chave: Biotechnology; Chemistry; Ecology; Earth & Environment.
Ano: 2010 URL: http://precedings.nature.com/documents/4167/version/1
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Asian soybean rust in Brazil: past, present, and future PAB
Godoy,Cláudia Vieira; Seixas,Claudine Dinali Santos; Soares,Rafael Moreira; Marcelino-Guimarães,Franscismar Correa; Meyer,Maurício Conrado; Costamilan,Leila Maria.
Abstract: Asian soybean rust, caused by the fungus Phakopsora pachyrhizi, is the most severe disease of the crop and can cause yield losses of up to 90%. The disease was first reported in Brazil in 2001. Epidemics of the disease are common in the country, where the fungus can survive year-round. Regulatory measures to reduce the inoculum between seasons and avoid late-season soybean have been adopted to manage the disease. Disease control has relied mainly on chemical control, but a lower sensibility of the fungus to fungicides has been reported in Brazil. Major-resistance genes have been mapped and incorporated into the cultivars. With the reduced efficacy of the fungicides, the adoption of integrated measures to control the disease will be important for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Phakopsora pachyrhizi; Biotechnology; Chemical control; Genetic resistance; Losses; Management..
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016000500407
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Assessment of Biotechnology Policies and International Trade in Key Markets for U.S. Agriculture AgEcon
Marchant, Mary A.; Song, Baohui.
The United States leads the world in agricultural biotechnology research, adoption, commercialization, and exports. Our biotech commodities are highly dependent on international markets. Thus, any biotech policy changes by key importing countries may affect U.S. agricultural biotech product exports. This article identifies key markets for U.S. agricultural exports including biotech commodities and discusses current and proposed biotech policies in key markets for U.S. agricultural exports focusing on Canada, Mexico, Japan, the European Union (EU), and China. Among these markets, labeling of biotech products is voluntary in Canada and Mexico but is mandatory in Japan, the EU, and, most recently, in China. For the EU, U.S. corn exports were almost...
Tipo: Journal Article Palavras-chave: Biotechnology; Biotech policy; Corn; Cotton; International trade; Soybeans; U.S. agricultural exports; Q13; Q17; Q16; Q18.
Ano: 2005 URL: http://purl.umn.edu/43513
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Automacao e racionalizacao na micropropagacao industrial. Infoteca-e
FARI, M.; MELO, N. F. de.
Tendencias da mecanizacao na propagacao in vitro em larga escala, automacao das etapas dos metodos tradicionais de cultura de tecidos, novastecnologias baseadas na utilizacao de sistemas de propagacao em meio liquido, desenvolvimento de sistemas de cultivo in vitro alternativos, sistema CLONER: uma nova iniciativa de racionalizacao da bancada de micropropagacao.
Tipo: Artigo de divulgação na mídia (INFOTECA-E) Palavras-chave: Propagacao in vitro; In vitro propagation; Biotecnologia; Cultura de Tecido; Meristema; Micropropagação; Biotechnology; Micropropagation; Tissue culture.
Ano: 1996 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/131977
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Avaliacao do fluxo genico entre a cultivar de soja BR-16 transgenica e BR-16 nao-transgenica no ecossistema do Cerrado no Distrito Federal. Infoteca-e
ABUD, S.; SOUZA, P. I. M.; MOREIRA, C. T.; VIANNA, G. R.; ANDRADE, S. R. M.; RECH, E. L.; ARAGAO, F. J. L..
bitstream/CPAC-2009/24176/1/p2002_67.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E) Palavras-chave: Biotecnologia; Glycine Max; Melhoramento Genético Vegetal; Planta Transgênica; Soja.; Biotechnology; Plant breeding; Transgenic plants.; Soybeans.
Ano: 2004 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/567487
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Axillary shoot induction of mahogany (Swietenia macrophylla King). Repositório Alice
PINTO, F.; DEGENHARDT-GOLDBACH, J.; QUOIRIN, M. G. G..
Resumo.
Tipo: Separatas Palavras-chave: Mahogany.; Biotecnologia; Mogno; Swietenia Macrophylla.; Biotechnology; Chlorosis..
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/974363
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Bacterial diversity dynamics in microbial consortia selected for lignin utilization. Repositório Alice
MENDES, I. V.; GARCIA, M. B.; BITENCOURT, A. C. A.; SANTANA, R. H.; LINS, P. de C.; SILVEIRA, R.; SIMMONS, B. A.; GLADDEN, J. M.; KRUGER, R. H.; QUIRINO, B. F..
Abstract:Lignin is nature?s largest source of phenolic compounds. Its recalcitrance to enzymatic conversion is still a limiting step to increase the value of lignin. Although bacteria are able to degrade lignin in nature, most studies have focused on lignin degradation by fungi. To understand which bacteria are able to use lignin as the sole carbon source, natural selection over time was used to obtain enriched microbial consortia over a 12-week period. The source of microorganisms to establish these microbial consortia were commercial and backyard compost soils. Cultivation occurred at two different temperatures, 30°C and 37°C, in defined culture media containing either Kraft lignin or alkaline-extracted lignin as carbon source. iTag DNA sequencing of...
Tipo: Artigo de periódico Palavras-chave: Biotecnologia; Carbono; Lignina; Composto Fenólico; Lignin; Phenolic compounds; Carbon; Energy conversion; Biotechnology; Degradation; Application technology.
Ano: 2021 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1134421
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Bacterias antárticas y agentes antibacterianos OceanDocs
Domínguez, M..
La Antártica se ha constituido en una región de gran interés científico debido a las condiciones extremas que allí se presentan, en especial, de temperatura y radiación ultravioleta. En el campo de la microbiología, las investigaciones se han enfocado, principalmente, en el estudio de la biodiversidad bacteriana, lo que ha llevado a la descripción de nuevas especies, y también gran interés ha despertado la producción de compuestos con aplicaciones biotecnológicas. Entre éstos se encuentran los que pudieran presentar actividad antibacteriana, de gran importancia debido a que uno de los mayores problemas de los últimos años es la emergencia de patógenos intrahospitalarios con una amplia resistencia a los agentes antibacterianos disponibles, no existiendo...
Tipo: Journal Contribution Palavras-chave: Bacteria; Biotechnology; Biotechnology; Bacteria; Http://aims.fao.org/aos/agrovoc/c_16165; Http://aims.fao.org/aos/agrovoc/c_765.
Ano: 2008 URL: http://hdl.handle.net/1834/3372
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Bark Extract of Bathysa cuspidata in the Treatment of Liver Injury Induced by Carbon Tetrachloride in Rats BABT
Gonçalves,Reggiani Vilela; Matta,Sérgio Luis Pinto da; Novaes,Rômulo Dias; Leite,João Paulo Viana; Peluzio,Maria do Carmo Gouveia; Vilela,Emerson Ferreira.
The aim of this study was to investigate the effect of bark extract Bathysa cuspidata (BCE) in the reconstitution of hepatic parenchyma and stroma after dysfunction induced by the CCl4. Liver lesions were induced by intraperitoneal administration of CCl4 (1 mL/kg) every 48 h for 12 days. The animals were treated with B. cuspidata extract (BCE) administered by gavage for another 12 days. Forty-nine rats were randomized into seven treatment groups with seven animals receiving CCl4, BCE (200, or 400 mg/kg) and the vehicle DMSO alone, or in different combinations. The extract alone showed no evidence of hepatic toxicity. In general, rats acutely exposed to CCl4 without the treatment with BCE presented high ALT and AST serum levels and high tissue content of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant defense; Biotechnology; Hepatotoxicity; Phytotherapy; Pathology.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400504
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BBSRC Data Sharing Policy Nature Precedings
Amanda Collis; David McAllister; Michael Ball.
BBSRC recognizes the importance of contributing to the growing international efforts in data sharing. BBSRC is committed to getting the best value for the funds we invest and believes that making research data more readily available will reinforce open scientific inquiry and stimulate new investigations and analyses. BBSRC supports the view that data sharing should be led by the scientific community and driven by scientific need. It should also be cost effective and the data shared should be of the highest quality. Members of the community are expected and encouraged to practice and promote data sharing, determine standards and best practice, and create a scientific culture in which data sharing is embedded.
Tipo: Manuscript Palavras-chave: Biotechnology; Developmental Biology; Genetics & Genomics; Immunology; Microbiology; Molecular Cell Biology; Neuroscience; Pharmacology; Bioinformatics; Plant Biology; Evolutionary Biology; Data Standards.
Ano: 2011 URL: http://precedings.nature.com/documents/6015/version/1
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Beyond the fuzzy lock-and-key: spontaneous symmetry shifts and glycan/lectin logic gates Nature Precedings
Rodrick Wallace.
Changes in the molecular topology of glycan/lectin interaction may explain observed reaction punctuation driven by experimental gradients in reactant concentration. Adoption of a 'biological renormalization' perspective from statistical physics for the analysis of such phase transitions suggests, in marked contrast to conventional physical systems, a broad spectrum of possible universality class behaviors. This spectrum may, in typical perverse biological manner, be of central scientific interest. Generalization, via formalism abducted from coevolutionary theory, suggests that glycan/lectin molecular switches instantiate logic gates that may be as sophisticated as those characterizing basic neural process, if on a different scale.
Tipo: Manuscript Palavras-chave: Biotechnology; Immunology; Microbiology; Molecular Cell Biology; Pharmacology; Evolutionary Biology.
Ano: 2012 URL: http://precedings.nature.com/documents/6849/version/1
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BioBrick Promoter Measurement Kit Instruction Sheets Nature Precedings
Jason Kelly.
Instructions for inserting a test promoter into the measurement kit plasmid for characterization as well as instructions for measuring the activity of the test promoter in Standard Promoter Units (SPUs). These instructions were distributed to the teams participating in the International Genetically Engineered Machines competition (iGEM).
Tipo: Manuscript Palavras-chave: Biotechnology; Microbiology; Molecular Cell Biology.
Ano: 2008 URL: http://precedings.nature.com/documents/2028/version/1
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Biochemical markers for nitrogen use efficience in maize. Repositório Alice
PURCINO, A. A. C.; SILVA, M. R. e; PAIVA, E.; ANDRADE, S. R. M..
Tipo: Parte de livro Palavras-chave: Marcadores bioquimia; Maize.; Biotecnologia; Milho; Nitrogênio; Zea Mays.; Biotechnology; Nitrogen..
Ano: 1993 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/482775
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