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Caminhos da Biotecnologia da Reprodução em Suínos. Infoteca-e
SOUZA, A. P. de; SILVA, Z.; SUPERTI, B. F. V.; MARQUES, M. G..
Artigo descreve algumas das biotecnologias que estão aprimoradas e podem abrir novas possibilidades para a reprodução na espécie suína.
Tipo: Artigo de divulgação na mídia (INFOTECA-E) Palavras-chave: Suinocultura; Biotecnologia; Reprodução animal; Animal reproduction; Swine; Biotechnology.
Ano: 2016 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1061837
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Can bacteria adapt to starvation-free environment? Nature Precedings
Kei Kitahara.
Bacteria will experience starvation-free environment if infinite nutrition is supplied continuously for a long period. In this study, an evolutionary experiment was performed for 118 days where bacteria adapted to starvation-free environment and reduced their doubling time. It is anticipated that this finding will help to select bacterial strains that can grow more rapidly in rich media.
Tipo: Manuscript Palavras-chave: Biotechnology; Genetics & Genomics; Microbiology; Evolutionary Biology.
Ano: 2012 URL: http://precedings.nature.com/documents/7017/version/1
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Can Technology Deliver on the Yield Challenge to 2050? AgEcon
Fischer, R.A.; Byerlee, Derek R.; Edmeades, Gregory O..
This paper was produced for the FAO Expert Meeting on How to Feed the World in 2050 (Rome, 24-26 June 2009).
Tipo: Conference Paper or Presentation Palavras-chave: Technology; Productivity; Yield gaps; Investment in R&D; Biotechnology; Input efficiency; Research and Development/Tech Change/Emerging Technologies.
Ano: 2009 URL: http://purl.umn.edu/55481
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Cancer Clinical Trials Optimization and Pharmacogenomics Nature Precedings
I. C. Baianu.
A critical overview of recent clinical trials in cancer is presented focused on signaling pathways blockers or inhibitors with a view to developing successful clinical trials employing personalized cancer therapies. Rational, pharmacogenomic strategies in cancer trials should be adopted that include specific molecular targeting based on adequate data for, and detailed modeling of, cancer cell genomes, modifications of cancer signaling pathways and epigenetic mechanisms. Novel translational oncogenomics research is rapidly expanding through the application of highly sensitive and specific advanced technology, research findings and computational tools and complex models to both pharmaceutical and clinical problems. Multiple sample analyses from several...
Tipo: Manuscript Palavras-chave: Biotechnology; Cancer; Chemistry; Genetics & Genomics; Immunology; Molecular Cell Biology; Pharmacology; Bioinformatics.
Ano: 2012 URL: http://precedings.nature.com/documents/7046/version/1
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Cancer invasion associated gene expression signature is present in differentially expressed genes in the reprogramming of fibroblasts into stem cells Nature Precedings
Wei-yi Cheng; Hoon Kim; Jessica Kandel; Dimitris Anastassiou.
Tumors become invasive by penetrating adjacent connective tissue, but the underlying biological mechanisms remain obscure. We recently identified a precise gene expression signature of fibroblastic origin associated with cancer invasion, the first step of the metastatic cascade. The signature contains many coordinately overexpressed genes, prominent among which are COL11A1, THBS2 and INHBA. Here we show that there is a striking similarity between the set of expressed genes in this metastasis-associated fibroblastic (MAF) signature and the set of genes that are downregulated when fibroblasts are reprogrammed to induced pluripotent stem cells (iPSCs). Because it is known that fibroblast reprogramming involves a mesenchymal epithelial transition (MET), the...
Tipo: Manuscript Palavras-chave: Biotechnology; Cancer; Developmental Biology; Bioinformatics.
Ano: 2011 URL: http://precedings.nature.com/documents/5924/version/1
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Caracterização molecular e bioquímica de populações de milho indígena (Zea mays L.). Repositório Alice
GUIMARAES, C. T.; VASCONCELOS, M. J. V.; BARROS, E. G.; PAIVA, E..
Tipo: Parte de livro Palavras-chave: Maize.; Biotecnologia; Biologia Molecular; Milho; Zea Mays.; Biotechnology; Molecular biology..
Ano: 1994 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/478519
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Caracterizacao molecular e estabelecimento de colecao de trabalho de Stylosanthes macrocephala com base em marcadores RAPD. Infoteca-e
FALEIRO, F. G.; BARROS, A. M.; FALEIRO, A. S. G.; CORDEIRO, M. C. R.; KARIA, C. T.; GOMES, A. C.; ANDRADE, R. P..
bitstream/CPAC-2009/24949/1/p2004_03.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E) Palavras-chave: RAPD; Feed legumes.; Biologia Molecular; Biotecnologia; Marcador Molecular; Leguminosa Forrageira; Variação Genética.; Genetic markers; Genetic variation; Biotechnology; Molecular biology..
Ano: 2004 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/567912
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Carbon Orientation in the Diatom Phaeodactylum tricornutum: The Effects of Carbon Limitation and Photon Flux Density ArchiMer
Heydarizadeh, Parisa; Veidi, Brigitte; Huang, Bing; Lukomska, Ewa; Wielgosz-collin, Gaetane; Couzinet-mossion, Aurélie; Bougaran, Gael; Marchand, Justine; Schoefs, Benoit.
Diatoms adapt to changing environmental conditions in very efficient ways. Among the mechanisms that can be activated, the reorientation of carbon metabolism is crucial because it allows the storage of energy into energy-dense molecules, typically lipids. Beside their roles in physiology, lipids are commercially interesting compounds. Therefore studies dealing with this topic are relevant for both basic and applied science. Although the molecular mechanisms involved in the reorientation of carbon metabolism as a response to a deficiency in nutrients such as nitrogen or phosphorus has been partially elucidated, the impacts of carbon availability on the implementation of the reorientation mechanisms remain unclear. Indeed, it has not been determined if the...
Tipo: Text Palavras-chave: Diatom; Carbon deficiency; Carbon metabolism; Stress; Light intensity; Regulation; Biotechnology; Phosphoenolpyruvate.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00489/60058/63338.pdf
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Category of Metabolic-Replication Systems in Biology and Medicine Nature Precedings
I. C. Baianu.
Metabolic-repair models, or (M,R)-systems were introduced in Relational Biology by Robert Rosen. Subsequently, Rosen represented such (M,R)-systems (or simply MRs)in terms of categories of sets, deliberately selected without any structure other than the discrete topology of sets. Theoreticians of life's origins postulated that Life on Earth has begun with the simplest possible organism, called the primordial. Mathematicians interested in biology attempted to answer this important question of the minimal living organism by defining the functional relations that would have made life possible in such a minimal system- a grandad and grandma of all living organisms on Earth.
Tipo: Manuscript Palavras-chave: Biotechnology; Microbiology; Neuroscience; Bioinformatics; Earth & Environment; Evolutionary Biology.
Ano: 2012 URL: http://precedings.nature.com/documents/7045/version/1
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Cattle farmers: profile and speech content analysis while undergoing training to adopt artificial insemination in Goiás State, Brazil R. Bras. Zootec.
Gordo,João Maurício Lucas; Silva,Marcelo Corrêa da; Solano,Gianice de Almeida; Lopes,Fernando Brito; Costa,Marcos Fernando Oliveira e; Rocha,Francisco Eduardo de Castro; Fioravanti,Maria Clorinda Soares; Sereno,José Robson Bezerra.
This study was undertaken to verify major issues present in the discourse of cattle farmers when discussing the use of artificial insemination (AI). Seventy-one beef and dairy cattle farmers in the state of Goiás, Brazil, were interviewed using semi-structured questionnaires during AI training courses in 2010 and 2011. Data analyses were done using the classic method of textual speech content analysis, performing the pre-analytical, analytical and inferential stages. The written questions were separated in elementary context units (ECU) and sorted into similar thematic groups. These groups originated categories and subcategories, which were nominated based on an a posteriori criteria. Overall, personal motivation was the main issue characterizing discourse...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Animal reproduction; Biotechnology; Discourse analysis; Social psychology.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982013000300003
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Causal Stability Ranking Nature Precedings
Daniel J. Stekhoven; Lars Hennig; Gardar Sveinbjörnsson; Izabel Moraes; Marloes H. Maathuis; Peter Bühlmann.
Genotypic causes of a phenotypic trait are typically determined via randomized controlled intervention experiments. Such experiments are often prohibitive with respect to durations and costs. We therefore consider inferring stable rankings of genes, according to their causal effects on a phenotype, from observational data only. Our method allows for efficient design and prioritization of future experiments, and due to its generality it is useable for a broad spectrum of applications.
Tipo: Manuscript Palavras-chave: Biotechnology.
Ano: 2011 URL: http://precedings.nature.com/documents/6716/version/1
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Cell Proteomic Footprint Nature Precedings
Petr Lokhov; Elena Balashova; Maxim Dashtiev.
The authentication of mammalian cell cultures and their subpopulations are of tremendous demand in biotechnology and cell therapy. However, current techniques are either not efficient or can be very complex and expensive. Here we report a simple and straightforward approach for authentication of biological cells and their subpopulations with high speed, high throughput, low sample cost, and high sensitivity. We discovered that cell cultures treated with protease at soft, “non-killing” conditions release fragments of cell surface proteins, which composition is a strong characteristic of the cells. Mass spectrometric analysis of the released fragments allows a direct comparison of the produced mass spectrum with the mass spectrum of...
Tipo: Manuscript Palavras-chave: Biotechnology.
Ano: 2008 URL: http://precedings.nature.com/documents/2091/version/1
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Cell-free Embryonic Stem Cell Extract-mediated Derivation of Multi-potent Stem Cells from NIH3T3 Fibroblasts for Functional and Anatomical Ischemic Tissue Repair Nature Precedings
Johnson Rajasingh; Hiromichi Hamada; Evelyn Bord; Tina Thorne; Ilona Goukassian; Erin Lambers; Prasanna Krishnamurthy; Gangjian Qin; Karen Schlauch; Kenneth M. Rosen; Deepali Ahluwalia; Yan Zhu; Douglas W. Losordo; Raj Kishore.
The oocyte-independent generation of multipotent stem cells is one of the goals in regenerative medicine. We report that upon exposure to mouse ES cell (ESC) extracts, reversibly permeabilized NIH3T3 cells undergo de-differentiation followed by stimulus-induced re-differentiation into multiple lineage cell types. Genome-wide expression profiling revealed significant differences between NIH3T3 and ESC-extract treated NIH3T3 cells including re-activation of ESC specific transcripts. Epigenetically, ESC extracts induced CpG de-methylation of Oct4 promoter, hyper-acetylation of histones 3 and 4 and decreased lysine 9 (K-9) dimethylation of histone 3. In mouse models of surgically-induced hind limb ischemia (HLI) or acute myocardial infarction (AMI)...
Tipo: Manuscript Palavras-chave: Biotechnology.
Ano: 2007 URL: http://precedings.nature.com/documents/1208/version/1
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Cellular delivery of antibodies: effective targeted subcellular imaging and new therapeutic tool Nature Precedings
Marzia Massignani; Irene Canton; Nisa Patikarnmonthon; Nicholas J. Warren; Steven P. Armes; Andrew L. Lewis; Giuseppe Battaglia.
It is already more than a century since the pioneering work of the Nobel Laureate Ehrlich gave birth to the side chain theory1, which helped to define antibodies and their ability to target specific biological sites. However, the use of antibodies is still restricted to the extracellular space due to the lack of a suitable delivery vehicle for the efficient transport of antibodies into live cells without inducing toxicity. In this work, we report the efficient encapsulation and delivery of antibodies into live cells with no significant loss of cell viability or any deleterious affect on the cell metabolic activity. This delivery system is based on poly(2-(methacryloyloxy)ethyl phosphorylcholine)-block-(2-(diisopropylamino)ethyl methacrylate), (PMPC-PDPA),...
Tipo: Manuscript Palavras-chave: Biotechnology; Chemistry; Immunology; Pharmacology.
Ano: 2010 URL: http://precedings.nature.com/documents/4427/version/1
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CENARGEN, función e importancia. Infoteca-e
VALOIS, A. C. C..
Tipo: Artigo de periódico Palavras-chave: CENARGEN; Biotecnologia; Instituição de Pesquisa; Recurso Genético; Biotechnology; Genetic resources; Research institutions.
Ano: 1996 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/171558
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CFP and YFP photostabilities are differentially affected by common mounting fluids Nature Precedings
Johannes A. Schmid.
The use of spectrally distinct variants of green fluorescent protein (GFP) such as cyan or yellow mutants (CFP and YFP, respectively) is very common in all different fields of life sciences, e.g. for marking and tracing of specific proteins or cells or to determine protein interactions. In the later case, the quantum physical phenomenon of fluorescence resonance energy transfer (FRET) is visualized by specific microscopy techniques. When we applied a commonly used FRET microscopy technique - the increase in CFP-fluorescence after bleaching of YFP, we noticed that it worked well for live cells, but that most of the FRET-signal was lost in fixed cells mounted in commercial microscopy mounting fluids. Subsequently, we could show that CFP bleached much faster...
Tipo: Manuscript Palavras-chave: Biotechnology; Molecular Cell Biology.
Ano: 2010 URL: http://precedings.nature.com/documents/4517/version/1
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Changes in Antioxidant Enzymes Activity and Oxidative Damage in Four Argania spinosa Ecotypes Under Water Stress Conditions Nature Precedings
Abdelghani Chakhchar; Abderrahim Ferradous; Mouna Lamaoui; Said Wahbi; Cherkaoui El Modafar.
_Argania spinosa_ L. Skell, a tree endemic of Morocco, grows in semiarid and arid areas. Biochemical response to drought in four ecotypes of the _A. spinosa_ was compared. Choice of ecotypes was based on evident differences in geographical features in south-west Morocco: two coastal ecotypes (Essaouira and Agadir) and two paralittorale ecotypes (Aoulouz and Lakhssas). Lipid peroxidation (malonyldialdehyde (MDA)), content of endogenous hydrogen peroxide (H2O2) and antioxidant enzyme system (Catalase (CAT), Ascorbate peroxidase (APX), Total peroxidase (POD) and Polyphenoloxidase (PPO) were measured in control (100% relative humidity (RH) at field capacity (FC)) and water-stressed plants (50 and 25% RH at FC). For all ecotypes, drought stress significantly...
Tipo: Poster Palavras-chave: Biotechnology; Plant Biology.
Ano: 2011 URL: http://precedings.nature.com/documents/6189/version/1
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Chapter 07: USING CONJOINT ANALYSIS TO ASSESS CONSUMERS' ACCEPTANCE OF PST-SUPPLEMENTED PORK AgEcon
Halbrendt, Catherine K.; Pesek, John D., Jr.; Parsons, April; Lindner, Robert K..
This book was originally published by Westview Press, Boulder CO, 1995.
Tipo: Book Chapter Palavras-chave: Biotechnology; Pork products; Conjoint analysis; Willingness to buy; Food Consumption/Nutrition/Food Safety.
Ano: 1995 URL: http://purl.umn.edu/25973
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Characterization of a pig skeletal muscle microarray to study pork quality: the GenmascqChip 15K Nature Precedings
Marie Damon; Frédéric Herault; Annie Vincent; Pascale Le Roy; Pierre Cherel.
Pork quality is highly variable and availability of relevant predictors of fresh meat quality at slaughter is critical to optimize carcass use and improve its valuation. High throughput gene expression studies have been widely used to describe biological mechanisms underlying variation of several traits but data are scarce regarding their use as tools for development of biomarkers. The GENMASCQ (GENomics enabled Marker Assisted Selection and Certification of Quality in Pork products ) research program was set up to select, using high-throughput gene expression screening, markers of the biological variation mostly appropriate to describe, and ultimately forecast, the sensory quality of an important meat product, accounting for nearly 40 % of meat protein...
Tipo: Manuscript Palavras-chave: Biotechnology; Genetics & Genomics; Bioinformatics.
Ano: 2011 URL: http://precedings.nature.com/documents/5730/version/1
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Characterization of the regulatory region of bovine mucin 2 (MUC2) gene. Repositório Alice
MELO, E. de O.; YAMASHITA, M. S. A..
Na publicação: E. O. Melo.
Tipo: Separatas Palavras-chave: Gene regulation; Gel-forming mucin; Gastrointestinal tract; Biotechnology.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1100973
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