Consulta: subjectFacets:"Cardiomyocytes"
Registros recuperados: 5
Data/hora: 27/07/2024 02:55:33

Ca2+/Calmodulin-dependent kinase II delta B is essential for cardiomyocyte hypertrophy and complement gene expression after LPS and HSP60 stimulation in vitro
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Cruz Junho,C.V.; Trentin-Sonoda,M.; Alvim,J.M.; Gaisler-Silva,F.; Carneiro-Ramos,M.S.
Inflammation plays an important role in the development of cardiovascular diseases (CVDs), suggesting that the immune system is a target of therapeutic interventions used for treating CVDs. This study evaluated mechanisms underlying inflammatory response and cardiomyocyte hypertrophy associated with bacterial lipopolysaccharide (LPS)- or heat shock protein 60 (HSP60)-induced Toll-like receptor (TLR) stimulation and the effect of a small interfering RNA (siRNA) against Ca2+/calmodulin-dependent kinase II delta B (CaMKIIδB) on these outcomes. Our results showed that treatment with HSP60 or LPS (TLR agonists) induced cardiomyocyte hypertrophy and complement system C3 and factor B gene expression. In vitro silencing of CaMKIIδB prevented complement gene...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiomyocytes; Hypertrophy; Inflammation; Complement; TLR 2/4; CaMKII.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000700609

Exercise training and detraining modify the morphological and mechanical properties of single cardiac myocytes obtained from spontaneously hypertensive rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Carneiro-Júnior,M.A.; Pelúzio,M.C.G.; Silva,C.H.O.; Amorim,P.R.S.; Silva,K.A.; Souza,M.O.; Castro,C.A.; Roman-Campos,D.; Prímola-Gomes,T.N.; Natali,A.J.
We determined the effects of exercise training and detraining on the morphological and mechanical properties of left ventricular myocytes in 4-month-old spontaneously hypertensive rats (SHR) randomly divided into the following groups: sedentary for 8 weeks (SED-8), sedentary for 12 weeks (SED-12), treadmill-running trained for 8 weeks (TRA, 16 m/min, 60 min/day, 5 days/week), and treadmill-running trained for 8 weeks followed by 4 weeks of detraining (DET). At sacrifice, left ventricular myocytes were isolated enzymatically, and resting cell length, width, and cell shortening after stimulation at a frequency of 1 Hz (~25°C) were measured. Cell length was greater in TRA than in SED-8 (161.30 ± 1.01 vs 156.10 ± 1.02 μm, P &lt; 0.05, 667 vs 618 cells,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Physical activity; Inactivity; Hypertension; Cardiomyocytes.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001100005

Hydrogen sulfide protects H9c2 cardiomyoblasts against H2O2-induced apoptosis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zhang,You En; Huang,Guang Qing; Wu,Bing; Lin,Xin Duo; Yang,Wen Zi; Ke,Zun Yu; Liu,Jie.
Reactive oxygen species (ROS) are highly reactive chemical species that may cause irreversible tissue damage, and play a critical role in cardiovascular diseases. Hydrogen sulfide (H2S) is a gasotransmitter that acts as a ROS scavenger with cardio-protective effects. In this study, we investigated the cytoprotective effect of H2S against H2O2-induced apoptosis in cardiomyocytes. H9c2 rat cardiomyoblasts were treated with H2S (100 μM) 24 h before challenging with H2O2 (100 μM). Apoptosis was then assessed by annexin V and PI, and mitochondrial membrane potential was measured using a fluorescent probe, JC-1. Our results revealed that H2S improved cell viability, reduced the apoptotic rate, and preserved mitochondrial membrane potential. An increased Bcl-2 to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reactive oxygen species; ROS; Hydrogen sulfide; H2O2-induced apoptosis; Cardiomyocytes.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000400608

Intramyocardial implantation of differentiated rat bone marrow mesenchymal stem cells enhanced by TGF-β1 improves cardiac function in heart failure rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Lv,Y.; Liu,B.; Wang,H.P.; Zhang,L.
The present study tested the hypotheses that i) transforming growth factor beta 1 (TGF-β1) enhances differentiation of rat bone marrow mesenchymal stem cells (MSCs) towards the cardiomyogenic phenotype and ii) intramyocardial implantation of the TGF-β1-treated MSCs improves cardiac function in heart failure rats. MSCs were treated with different concentrations of TGF-β1 for 72 h, and then morphological characteristics, surface antigens and mRNA expression of several transcription factors were assessed. Intramyocardial implantation of these TGF-β1-treated MSCs to infarcted heart was also investigated. MSCs were initially spindle-shaped with irregular processes. On day 28 after TGF-β1 treatment, MSCs showed fusiform shape, orientating parallel with one...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone marrow mesenchymal stem cell; Transforming growth factor beta1; Cardiomyocytes; Differentiation; Intramyocardial implantation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000600603

Myostatin promotes distinct responses on protein metabolism of skeletal and cardiac muscle fibers of rodents
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Manfredi,L.H.; Paula-Gomes,S.; Zanon,N.M.; Kettelhut,I.C.
Myostatin is a novel negative regulator of skeletal muscle mass. Myostatin expression is also found in heart in a much less extent, but it can be upregulated in pathological conditions, such as heart failure. Myostatin may be involved in inhibiting protein synthesis and/or increasing protein degradation in skeletal and cardiac muscles. Herein, we used cell cultures and isolated muscles from rats to determine protein degradation and synthesis. Muscles incubated with myostatin exhibited an increase in proteolysis with an increase of Atrogin-1, MuRF1 and LC3 genes. Extensor digitorum longus muscles and C2C12 myotubes exhibited a reduction in protein turnover. Cardiomyocytes showed an increase in proteolysis by activating autophagy and the ubiquitin proteasome...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myostatin; Skeletal muscle; Cardiomyocytes; Protein degradation; Protein synthesis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001200604