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Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil 102
Fajardo,Thor V.M.; Dianese,Érico C.; Eiras,Marcelo; Cerqueira,Daniela M.; Lopes,Daniela B.; Ferreira,Marisa A.S.V.; Martins,Cláudia R.F..
Leafroll is an economically important disease affecting grapevines (Vitis spp.). Nine serologically distinct viruses, Grapevine leafroll-associated virus-1 through 9, are associated with this disease. The present study describes the coat protein gene sequence of four GLRaV-3 isolates occurring in the São Francisco River basin, Northeastern Brazil. The viral RNA was extracted from GLRaV-3 ELISA-positive plants and the complete coat protein gene was amplified by RT-PCR. Sequences were generated automatically and compared to the complete coat protein sequence from North American (NY1) and Chinese (Dawanhong Nº2 and SL10) GLRaV-3 isolates. The four studied isolates, named Pet-1 through 4, showed deduced amino acid identities of 98-100% (Pet-1 through 3) and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Closteroviridae; Ampelovirus; GLRaV-3.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582007000400008
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MOLECULAR VARIABILITY OF THREE GENES OF POTATO VEIN YELLOW VIRUS INFECTING Solanum tuberosum, USING SINGLE STRAND CONFORMATIONAL POLYMORPHISM 37
CUBILLOS-ABELLO,Karen Andrea; GUZMÁN BARNEY,María Mónica.
Potato yellow vein virus (PYVV), a virus with tripartite RNA (ss+) genome is classified as member of the genus Crinivirus within the family Closteoviridae. PYVV is the causal agent of potato yellow vein disease (PYVD) with yield loss between 25 %-50 % in field. Single strand conformational polymorphism (SSCP) has been reported to estimate variability in different viruses' species. In this study, the molecular variability of PYVV analyzed by SSCP patterns of three genes: major capsid protein (CP), minor capsid protein (CPm), and heat shock protein (Hsp70) obtained from 60 virus isolates from potato plants expressing PYVD. Leaves collected in Nariño, Colombia from 30 Solanum tuberosum Phureja Group (PhG) and 30 from the Andigena Group (AG). Genes amplified...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Closteroviridae; Potato; Variability; Viral proteins.
Ano: 2015 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2015000100026
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