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Temporal expression pattern of myostatin transcripts during chicken embryogenesis Arq. Bras. Med. Vet. Zootec.
Gabriel,J.E.; Alvares,L.E.; Paz,C.C.P.; Packer,I.U.; Coutinho,L.L..
No presente estudo, estimou-se a abundância dos transcritos da miostatina foi estimada durante a embriogênese de galinha por análises de RT-PCR competitiva. Níveis basais de mRNA desse gene foram detectados até o estádio HH15, enquanto acúmulos significativos nesses níveis foram observados apenas no estádio HH24, seguido por redução na abundância desses transcritos a partir do estádio HH26. Tais descobertas preliminares proporcionam informações relevantes sobre a ativação do fator de crescimento miostatina durante o desenvolvimento in ovo de aves.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Chicken embryo; Myostatin; Temporal expression; MRNA abundance; Competitive RT-PCR.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000500033
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Effect of growth hormone on fatty acid synthase gene expression in porcine adipose tissue cultures Genet. Mol. Biol.
José,Andrea A.F.B.V.; Alvares,Lúcia. E.; Delgado,Eduardo F.; Coutinho,Luiz L.; Lanna,Dante P.D..
We describe an efficient in vitro assay to test growth hormone effects on mRNA levels and fatty acid synthase (FAS, EC. 2.3.1.85) activity. Swine adipose tissue explants were long-term cultured in medium containing growth hormone and FAS mRNA levels and enzyme activity were measured. We quantified FAS transcripts by competitive reverse transcriptase PCR (RT-PCR) using total RNA from cultured adipose tissue explants and RT-PCR standard-curves were constructed using a cloned 307 bp segment of native FAS cDNA and a shorter fragment from which a 64 bp (competitor, 243 bp) internal sequence had been deleted. A known amount of competitor was added to each PCR as an internal control and µ-actin transcripts were also measured to correct for differences in total...
Tipo: Info:eu-repo/semantics/article Palavras-chave: MRNA quantification; Enzyme activity; Swine; Cultures of adipose tissue explants; Competitive RT-PCR.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100032
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Temporal expression pattern of the insulin-like growth factor II and fibroblast growth factor transcripts in avian embryogenesis BABT
Gabriel,Jane Eyre; Alvares,Lúcia Elvira; Coutinho,Luiz Lehmann.
In this study, the abundance of IGF-II and bFGF transcripts was estimated in the chicken embryos using the competitive RT-PCR analysis. Significant enhancements in the abundance of IGF-II mRNA were observed at stages HH1 and 5, and a new accumulation in these levels was observed at stage HH18 in comparison to the basal levels. The abundance of bFGF mRNA increased significantly at stages HH18 and 20, followed by an upregulation in the expression of these transcripts at stage HH26. These findings provided important information about the temporal expression pattern of IGF-II and bFGF transcripts in the whole chicken embryos during in ovo development.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chicken embryos; Temporal expression; Competitive RT-PCR; Growth factors.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500011
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