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Human FcRn can mediate the transport across intestinal mucosal barrier and prolong the half-life of rabbit IgG in vivo BABT
Pang,Guangchang; Wang,Yufang; Xie,Junbo; Chen,Qingsen; Hu,Zhihe.
FcRn (neonatal Fc receptor) plays an important role in IgG transportation, antigen presentation and signal transmission. In this study, the complement fixation test and flow cytometry test were performed to verify whether the heterologous antibody could be transmitted to the serum or leukocyte with FcγR (Fc gamma receptor) across the intestinal mucosa. The results showed that rabbit anti-bovine IgG could be detected in both the serum and the leukocytes, which indicated that the heterologous antibody could transport across the intestinal mucosa to enter the blood and be effectively delivered to the leukocytes with FcγR. In addition, the results also showed that the rabbit anti-bovine IgG still could be detected in the leukocyte group (P=0.044<0.05) after...
Tipo: Info:eu-repo/semantics/article Palavras-chave: FcRn; Heterogeneous IgG transport; Complement fixation test; FcγR; Gastrointestinal barrier.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300387
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Evaluation of dot-blot test for serological diagnosis of bovine brucellosis BJM
Bastos,Carla Resende; Mathias,Luis Antonio; Jusi,Márcia Mariza Gomes; Santos,Renata Ferreira dos; Silva,Glaucenyra Cecília Pinheiro da; André,Marcos Rogério; Machado,Rosangela Zacarias; Bürger,Karina Paes.
Abstract The objective of this study was to standardize and validate the dot-blot test for the serological diagnosis of bovine brucellosis, compare the results with those found in the 2-mercaptoethanol (2-ME) and complement fixation test (CF), and estimate the relative sensitivity and specificity of the dot-blot compared to these tests. Fifty bovine blood serum samples were used for the test standardization, and 1315 samples were used for evaluation and comparison between the tests; the results were compared using the Kappa indicator. At the end of standardization, it was established as optimal for the antigen obtained from Brucella abortus B19 after passing through a microorganism rupture process, the blood serum samples diluted at 1:100, and the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brucella abortus; Animal health; Zoonoses; 2-Mercaptoethanol; Complement fixation test.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300564
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