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The aminoglycoside antibiotic gentamicin is able to alter metabolic activity and morphology of MDCK-C11 cells: a cell model of intercalated cells BJMBR
Coutinho,A.G.G.; Biscaia,S.M.P.; Fernandez,R.; Tararthuch,A.L..
It is well known that the aminoglycoside antibiotic gentamicin is capable of causing damage to kidney cells. Given the known involvement of Ca2+ in the nephrotoxic action of gentamicin, the purpose of this study was to establish a relationship between the concentration of intracellular Ca2+ ([Ca2+]i) and cellular cytotoxicity using MDCK-C11 cells, a clone that has several properties that resemble those of intercalated cells of the distal nephron. Changes in [Ca2+]i was determined using fluorescence microscopy. Cell viability was evaluated by the neutral red method, and cell cytotoxicity by the MTT method. The [Ca2+]i gradually increased when cells were exposed to 0.1 mM gentamicin for 10, 20, and 30 min. The presence of extracellular Ca2+ was found to be...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gentamicin; [Ca2+]i; MDCK-C11 cells; Cytotoxicity.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001000610
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The cytotoxic and antileishmanial activity of extracts and fractions of leaves and fruits of Azadirachta indica (A Juss.) Biol. Res.
Carneiro,Sabrina M. P; Carvalho,Fernando A. A; Santana,Lorena C. L. R; Sousa,Alessian P. L; Neto,José M. M; Chaves,Mariana H.
The leishmaniases are severe parasitic diseases that occur worldwide, caused by protozoa of the genus Leishmania. Studies with medicinal plants can lead to a range of possibilities for treating and improving the patients' quality of life. Research on Azadirachta indica fractions and extracts has shown that they have excellent anti-leishmanial activity based on bioactivity-guided fractionation of ethanolic extracts of leaves and seeds and in vitro activity against promastigotes. In this research the most efficient extracts and fractions were selected for tests on intracellular amastigotes of Leishmania amazonensis. The ethanolic extract of the leaves and dichloromethane and chloroform fractions had IC50 values of 38, 3.9 and 1.2 μg/mL for...
Tipo: Journal article Palavras-chave: Anti-leishmanial; Azadirachta indica; Cytotoxicity; Leishmaniasis; Leishmania amazonensis.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200002
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The cytotoxicity of methacryloxylethyl cetyl ammonium chloride, a cationic antibacterial monomer, is related to oxidative stress and the intrinsic mitochondrial apoptotic pathway BJMBR
Sai,Ma; Le-qun,Shan; Yu-hong,Xiao; Fang,Li; Li,Huang; Shen,Lijuan; Ji-hua,Chen.
Antibacterial monomers incorporated in dentin bonding systems may have toxic effects on the pulp. Thus, the cytotoxicity of antibacterial monomers and its underlying mechanisms must be elucidated to improve the safety of antibacterial monomer application. The influence of an antibacterial monomer, methacryloxylethyl cetyl ammonium chloride (DMAE-CB), on the vitality of L929 mouse fibroblasts was tested using MTT assay. Cell cycle progression was studied using flow cytometry. Production of intracellular reactive oxygen species (ROS) after DMAE-CB treatment was measured using 2,7-dichlorodihydrofluorescein diacetate staining and flow cytometry analysis. Loss of mitochondrial membrane potential, disturbance of Bcl-2 and Bax expression, as well as release of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Quaternary ammonium compounds; Dental monomer; Cytotoxicity; Apoptosis; Reactive oxygen species; Methacryloxylethyl cetyl ammonium chloride.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001100008
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The investigation of cytogenetic and oxidative effects of diffractaic acid on human lymphocyte cultures BABT
Demir,Leyla; Toğar,Başak; Türkez,Hasan; Sozio,Piera; Aslan,Ali; Stefano,Antonio Di.
Diffractaic acid (DA) is a naturally occurring depside derivative found in several lichen species. It has a wide range of important biological effects such as analgesic and antiviral properties, although its cytotoxic, cytogenetic and oxidative effects have not been investigated in human blood tissue yet. Therefore, increasing concentrations (1, 5, 10, 25, 50, 100 and 200 mgL-1) of DA was added into human whole blood cultures. 3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyl tetrazolium bromide (MTT) assay was used to assess the cell viability and/or cytotoxicity and genotoxic damage potential of DA using chromosome aberration (CA) and micronucleus (MN) tests were performed. In addition, oxidative alterations were determined by the total antioxidant capacity...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diffractaic acid; Cytotoxicity; Human blood; Genotoxicity; Oxidative status.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100075
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The Risk Evaluation of Tungsten Oxide Nanoparticles in Cultured Rat Liver Cells for Its Safe Applications in Nanotechnology BABT
Turkez,Hasan; Sonmez,Erdal; Turkez,Ozlem; Mokhtar,Yousef Ibrahem; Stefano,Antonio Di; Turgut,Guven.
Tungsten (VI) oxide (WO3) nanoparticles (NPs) are used for many industrial purposes in everyday life. However, their effects on human health have not been sufficiently evaluated. Therefore, the present study was designed to investigate the toxicity potentials of various concentrations (0 to 1000 ppm) of WO3NPs (<100 nm particle size) in cultured primary rat hepatocytes. The results of cell viability assay showed that the higher concentrations of dispersed WO3 NPs (300, 500 and 1000 ppm) caused significant (p<0.05) decreases of cell viability. Also, dose dependent negative alterations were observed in oxidative status and antioxidant capacity levels after the application of WO3 in cultured rat primary hepatocytes. The results of genotoxicity tests...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tungsten oxide; Nanotoxicity; Genotoxicity; Cytotoxicity; Hepatocytes; Oxidative stress.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400532
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Thioglycollate-elicited murine macrophages are cytotoxic to Mycoplasma arginini-infected YAC-1 tumor cells BJMBR
Ribeiro-Dias,F.; Russo,M.; Nascimento,F.R.F.; Barbuto,J.A.M.; Timenetsky,J.; Jancar,S..
Macrophages are important components of natural immunity involved in inhibition of tumor growth and destruction of tumor cells. It is known that these cells can be activated for tumoricidal activity by lymphokines and bacterial products. We investigated whether YAC-1 tumor cells infected with Mycoplasma arginini stimulate nitric oxide (NO) release and macrophage cytotoxic activity. Thioglycollate-elicited macrophages from male BALB/c mice were co-cultured for 20 h with YAC-1 tumor cells infected or not with Mycoplasma arginini. The cytotoxic activity was evaluated by MTT assay and nitrite levels were determined with the Griess reagent. Thioglycollate-elicited macrophages co-cultured with noninfected YAC-1 cells showed low cytotoxic activity (34.7 ± 8.6%)...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Macrophages; Cytotoxicity; Mycoplasma; Tumor cells; Nitric oxide.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100010
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Torta de mamona destoxificada por diferentes produtos químicos alcalinos em dietas de cabras leiteiras e seu efeito sobre a presença ou não de lectinas citotóxicas no leite. Repositório Alice
SANTOS, R. D.; POMPEU, R. C. F. F.; SAMPAIO, A. E.; SALLES, H. O.; LUCAS, R. C.; XIMENES, L. V..
Com o crescimento da utilização de energias mais limpas, a participação do biodiesel e o interesse no cultivo e processamento da mamona (Ricinus communis L.), vem criando possibilidade da utilização de co-produtos resultantes do processo de extração do óleo, tendo a torta de mamona como principal subproduto, devendo ser transformada em um produto atóxico para que possa ser usado na alimentação animal. No entanto, o conhecimento prévio de atuação do princípio ativo do produto atóxico (torta) nas vias metabólicas dos animais, e as alterações nas características do produto final (carne e leite) ainda são fatores desconhecidos. Objetivou-se avaliar métodos de detecção de lectinas citotóxicas no leite de cabra visando a seleção de uma técnica capaz de...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Caprino leiteiro; Cabra Leiteira; Aglutinação; Eletroforese; Ricinus Communis; Dieta; Subproduto; Alimento Para Animal; Leite de Cabra; Dairy goats; Animal feeders; Industrial byproducts; Byproduct utilization; Goat milk; Cytotoxicity; Lectins.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102227
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Toxicity at the cellular level of artificial synthetic flavorings Biological Sciences
Sales, Ila Monize Sousa; Oliveira, Jussara Damascena de; Santos, Fabelina Karollyne Silva dos; Feitoza, Lidiane de Lima; Sousa, João Marcelo de Castro e; Peron, Ana Paula.
The goal of the present study was to evaluate the cytotoxicity and genotoxicity of artificial synthetic flavoring agents Cookie and Tutti-frutti. To this end, root meristem cells of Allium cepa L. were exposed to these substances in exposure times of 24 and 48 hours using individual doses of 0.3; 0.6 and 0.9 mL and doses combined as follows: 0.3 mL + 0.3 mL; 0.6 mL and 0.9 mL + 0.6 mL + 0.9 mL. After applying the treatments, root meristems were fixed, hydrolyzed, stained and analyzed a total of 5,000 cells using an optical microscope to evaluate each dose and combined treatment. All three doses of Cookie flavoring and combined treatments significantly inhibited cell division of the tissue studied. Doses of Tutti-Frutti caused no change in cell division...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Flavorings; Cell division; Cell aberrations; Cytotoxicity; Genotoxicity flavorings; Cell division; Cell aberrations; Cytotoxicity; Genotoxicity.
Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/30751
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Toxicity evaluation to mice of Phylloseptin-1, an antimicrobial peptide from the skin secretion of Phyllomedusa hypochondrialis (Amphibia). Repositório Alice
KÜCKELHAUS, S.; LEITE, J. R. S. A.; NEVES, M. P.; FROTA, K. S.; ABDALA, L. F.; JUNQUEIRA, M. I. M.; BLOCH JUNIOR, C.; TOSTA, C. E..
bitstream/item/178132/1/ID-27936-1.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Phylloseptin-1; Antimicrobial peptide; Phyllomedusa hypochondrialis; Mouse; Antimicróbico; Peptídeo; Rato; Cytotoxicity; Genotoxicity.
Ano: 2007 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/187694
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Toxicity in food flavorings at the cellular level associated with each other at different doses Biological Sciences
Silva, Sara Iolanda Oliveira da; Santana, Gleuvânia Marques; Sales, Ila Monize Sousa; Sousa, João Marcelo de Castro e; Peron, Ana Paula.
This study aimed to examine the cytotoxicity and genotoxicity of synthetic flavorings, nature identical, Chocolate, Strawberry and Condensed Milk. This evaluation was performed in root meristem cells of Allium cepa L., in exposure times of 24 and 48 hours and using doses of 0.2; 0.4 and 0.6 mL, in combination, in which one of the three doses of a flavoring was combined with a different dose of one of the two other flavor additives studied. Roots were fixed in Carnoy’s solution, hydrolyzed in hydrochloric acid, stained with acetic orcein and then analyzed, under light microscopy, 5,000 cells for each treatment. For data analysis, it was used Chi-square test at 5%. All the treatments with combinations between the flavorings Chocolate/Strawberry and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aroma and flavor additives; Cytotoxicity; Genotoxicity A. cepa Citogenética.
Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/30475
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Toxicity of the goji berry fruit associated with artificial excipients and dried without additives Biological Sciences
Moura, Débora Dayane Araújo de; Veloso, Cleidiane Josefa dos Santos; Oliveira, Valtânia Ana de; Silva, Maria Eduarda Sousa e; Peron, Ana Paula.
 The aim of this study was to evaluate the cytotoxic and genotoxic potential of goji berry fruit-based pharmaceutical powders obtained from three pharmaceutical laboratories. The product A was tested at concentrations of 0.012; 0.025 and 0.05 g mL-1, and B and C at concentrations 0.02; 0.04 and 0.08 g mL-1. It was also evaluated the tea of the dried goji berry fruit (non-additives) in the concentrations 0.035; 0.07 and 0.14 g mL-1 for comparison to the results obtained with powdered goji berry. Tea concentrations in the two exposure times did not cause inhibition of cell division nor cellular alterations to meristem tissues. For the industrialized goji products, all concentrations analyzed caused significant antiproliferative effect to the tissues...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lycium barbarum L.; Natural pharmaceutical product; Cytotoxicity; Genotoxicity; Meristematic tissue..
Ano: 2018 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/37844
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Trypan blue exclusion assay by flow cytometry BJMBR
Avelar-Freitas,B.A.; Almeida,V.G.; Pinto,M.C.X.; Mourão,F.A.G.; Massensini,A.R.; Martins-Filho,O.A.; Rocha-Vieira,E.; Brito-Melo,G.E.A..
Dye exclusion tests are used to determine the number of live and dead cells. These assays are based on the principle that intact plasma membranes in live cells exclude specific dyes, whereas dead cells do not. Although widely used, the trypan blue (TB) exclusion assay has limitations. The dye can be incorporated by live cells after a short exposure time, and personal reliability, related to the expertise of the analyst, can affect the results. We propose an alternative assay for evaluating cell viability that combines the TB exclusion test and the high sensitivity of the flow cytometry technique. Previous studies have demonstrated the ability of TB to emit fluorescence when complexed with proteins. According to our results, TB/bovine serum albumin and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trypan blue; Fluorescence; Flow cytometry; Cytotoxicity.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400307
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Two new mixed-ligand coordination polymers based on multi-N chelating ligand inhibit YAP expression and induce caspase-mediated spinal tumor cell apoptosis BJMBR
Sun,Yi Fu; Shao,Li Wei; Chen,Qi; Gao,Xu; Li,Fang; Wu,Chang Yan.
Two new coordination polymers [Zn (bdc)(bpybzimH2)](DMF)0.5 (1, H2bdc=1,4-dicarboxybenzene, bpybzimH2=6,6′-bis-(1H-benzoimidazol-2-yl)-2,2′-bipyridine, DMF=N,N-dimethylformamide) and [Co (bpybzimH2)(sbc)]H2O (2, H2sbc=4-mercaptobenzoic acid) have been successfully prepared under solvothermal conditions using the multi-N chelating organic ligand bpybzimH2 as the foundational building block. In addition, the Cell Counting Kit-8 assay was conducted to evaluate the anti-proliferation activity of compounds 1 and 2 against human spinal tumor cells OPM-2. The cell viability curves showed that the two compounds have anti-proliferation activity on spinal tumor cells, and the activity of compound 1 is higher than compound 2. The annexin V-FITC/PI assay and western...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coordination polymers; Glioma cell strains; Cytotoxicity; Apoptosis; Immunoblotting.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000500608
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Use of GC/MS to identify chemical constituents and cytotoxic activity of the leaves of Phoradendron mucronatum and Phoradendron microphyllum (Viscaceae) Anais da ABC (AABC)
BASTOS,ISLA V.G.A.; OLIVEIRA,TATIANE B. DE; RODRIGUES,MARIA D.; MILITÃO,GARDÊNIA C.G.; SILVA,TERESINHA G. DA; TURATTI,IZABEL C.C.; LOPES,NORBERTO P.; MELO,SEBASTIÃO J. DE.
ABSTRACT Phoradendron mucronatum and P. microphyllum are plants that found in tropical and subtropical areas, used in traditional medicine and popularly known as mistle-thrush. The aim of this study was to identify the chemical constituents of different leaf extracts from P. mucronatum and P. microphyllum and assess cytotoxic activity against strains from a human tumour cells. Extracts obtained with hexane, dichloromethane, chloroform and ethyl acetate from the leaves were analysed by gas chromatography coupled with mass spectrometry (GC-MS) and the cytotoxicity was assessed by the MTT method (bromide (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)). The tested human tumour cells were NCI-H292 (human pulmonar mucoepidermoid carcinoma), MCF-7...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytotoxicity; Gas Chromatography; Phoradendron microphyllum; Phoradendron mucronatum.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000300991
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Virulence and cytotoxicity of seafood borne Aeromonas hydrophila BJM
Illanchezian,Seethalakshmi; Jayaraman,SathishKumar; Manoharan,Muthu Saravanan; Valsalam,Saritha.
The present study was conducted to determine the virulence and cytotoxicity of Aeromonas hydrophila strains isolated from seafood samples collected from 5 major fish markets in Chennai, Tamil Nadu, India. Among 73 A. hydrophila strains isolated from fish and shrimp samples, 86.3% exhibited haemolysis, 78.1% produced slime, 98.63% produced protease and also demonstrated cytotoxicity on Vero cells. Cell shrinkage, detachment and rounding of Vero cells were recorded as cytotoxic changes. Only one strain did not show haemolysis, slime production, proteolytic activity and cytotoxicity on treatment with Vero cells. Positive correlation was observed between proteolytic activity and cytotoxicity irrespective of haemolytic activity of the strains. These results...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aeromonas hydrophila; Seafood; Virulence; Cytotoxicity; Vero cells.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400016
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Winery by-products: extraction optimization, phenolic composition and cytotoxic evaluation to act as a new source of scavenging of reactive oxygen species. Repositório Alice
MELO, P. S.; MASSARIOLI, A. P.; DENNY, C.; SANTOS, L. F. dos.; FRANCHIN, M.; PEREIRA, G. E.; VIEIRA, T. M. F. de S.; ROSALEN, P. L.; ALENCAR, S. M. de..
2015
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Cytotoxicity (MTT); Winery by-products; Response surface methodology (RSM); Reactive oxygen species (ROS); Anthocyanins; Cytotoxicity.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1023593
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