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Induction of the expression of defence genes in Carica papaya fruit by methyl jasmonate and low temperature treatments 69
Rivera-Domínguez,Marisela; Astorga-Cienfuegos,Karen Rosalinda; Tiznado-Hernández,Martin Ernesto; González-Aguilar,Gustavo Adolfo.
The defence mechanisms that are activated by methyl jasmonate (MJ) in fruits are not well understood. In this work, we studied the expression of defence genes in papaya fruit that are induced by the exposure to MJ and/or low temperatures. The papaya fruits ‘Maradol’ were randomly divided into two groups: one group was the untreated control and the other was treated with 10-4 M of MJ. Half of the fruits from each of the two groups were stored after treatment for 5 days at 5ºC and 2 days at 20ºC. We studied the expression levels of the pdf1.1 and pdf1.2 genes by amplification from expression libraries created from the pulp and skin tissues of the papaya fruit. As a reference, the mRNA level of the 18s ribosomal gene was used. In the skin...
Tipo: Journal article Palavras-chave: Cold treatment; Defence proteins; Genetic expression; Jasmonic acid methyl ester; Papaya fruit.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500006
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