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SANTOS, F. C.; CLEMENTE, A. da C. S.; CAIXETA, F.; ROSA, S. D. V. F. da. |
Coffee seeds begin to develop shortly after fertilization and can take 6 to 8 months to complete their formation, a period during which all the characteristics of the mature seed are determined, directly influencing physiological quality. However, little is known about the molecular mechanisms that act during coffee seed maturation. The objective of the current study was to analyze expression of the β-tubulin (TUB) and endo-β-mannanase (MAN) genes during different phases at the end of development and in different tissues of Coffea arabica seeds. The transcription levels of the TUB and MAN genes were quantified in a relative manner using qRT-PCR in whole seeds, and dissected into embryos and endosperms at different developmental stages.... |
Tipo: Artigo de periódico |
Palavras-chave: QRT-PCR; Coffea Arábica.; Developmental stages.. |
Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1039275 |
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