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Real-Time PCR optimization to identify environmental Vibrio spp. strains ArchiMer
Tall, Amadou; Teillon, Anna; Boisset, Claire; Delesmont, R.; Touron-bodilis, A.; Hervio-heath, Dominique.
Aims: To identify Vibrio vulnificus, Vibrio cholerae and Vibrio alginolyticus using standardized DNA extraction method and real-time PCR assays, among a large number of bacterial strains isolated from marine environment. Methods and Results: Methods for DNA extraction and real-time PCR were standardized to identify a large number of Vibrio spp. strains isolated through regular collection campaigns of environmental samples. Three real-time PCR assays were developed from a multiplex PCR, targeting V. vulnificus, V. cholerae and V. alginolyticus on the dnaJ gene. After testing their specificity, these systems were applied for the identification of 961 strains isolated at 22°C (446 strains) and 37°C (515 strains) in September 2009. The predominance of V....
Tipo: Text Palavras-chave: DnaJ; Identification; Real-time PCR; Vibrio alginolyticus; Vibrio cholerae; Vibrio vulnificus.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00088/19901/17562.pdf
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