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Assay of antioxidant potential of two Aspergillus isolates by different methods under various physio-chemical conditions 58
Arora,Daljit Singh; Chandra,Priyanka.
The objective of this work was to screen fungi isolated from soil of different areas of Punjab, India for antioxidant activity by dot blot assay and around 45% of fungal isolates demonstrated antioxidant potential. Two selected strains of Aspergillus spp (Aspergillus PR78 and Aspergillus PR66) showing quantitatively best antioxidant activity by DPPH assay were further tested for their reducing power, ferrous ion and nitric oxide ion scavenging activity, FRAP assay and total phenolic content. Different physio-chemical parameters were optimized for enhancement of the activity. This revealed stationary culture grown for 10 days at 25ºC at pH 7 to be the best for antioxidant activity. Sucrose in the medium as carbon source resulted in highest antioxidant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant potential; Aspergillus; Dot blot assay; DPPH assay; FRAP assay; Reducing power; Soil fungi.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300029
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