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| Registros recuperados: 61 | |
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| Kaercher,Fernanda; Siqueira,Marlon; Mercadante,Alessandro; Figueiredo,Tatiana; Weiss,Romildo Romualdo; Siqueira,Felipe; Kozicki,Luiz Ernandes. |
| The aim of this work was to prepare the mares for embryo transfer. In group 1 (G1,treated, n=15), recipient mares in anoestrus or in a transition period were treated with 5.0, 3.0 and 2.0 mg of estradiol cipionate at the days 0, 1 and 2 respectively, beginning at the day of ovulation (DO). From the fourth day on, the mares this group received long-acting progesterone weekly, up to the 120 day of gestation. At D8, the embryo was collected from the donor and transferred to the recipient. At D12, the ultrasonographyc diagnosis of pregnancy was carried out. The control group (G2, not treated, n=20) was formed by cycling recipient mares, displaying ovulation at each 2 to 3 days after the donors mare ovulation. The pregnancy rate was higher (p<0.05) in the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryo transfer; Cycling and no cycling mares; Long acting progesterone; Estradiol cipionate; Mares recipient. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600009 |
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| FONSECA, J. F. da; ZAMBRINI, F. N.; PRATES, J. F.; MARTINS, L. T.; CASTRO, A. C. R.; ESTEVES, L. V.; SOUZA-FABJAN, J. M. G.; BRANDÃO, F. Z.; GUIMARÃES, J. D.. |
| Abstract: Animal surgery procedures are being progressively restricted worldwide in the context of embryo transfer. In small ruminants, the needs for development of alternative and efficient non-surgical techniques for embryo transfer have been emphasized. Non-surgical embryo recovery is well consolidated in Brazil in cattle and goats, while in sheep it remains a challenge. The aim of this study was to check the efficiency of different ways of estradiol benzoate administration on cervix dilation and embryo recovery in synchronized Santa Inês ewes. Considering that Santa Inês sheep have up to 1.3 lambs we can conclude that it is possible to perform efficient non-surgical embryo recovery in non-superovulated synchronized Santa Inês ewes, regardless the way... |
| Tipo: Parte de livro |
Palavras-chave: Embryo recovery; Raça Santa Inês; Ewe.; Ovino; Ovelha; Hormônio; Transferência de embrião; Reprodução animal; Estradiol.; Sheep; Benzoates; Reproduction; Embryo transfer; Brazil.. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1070784 |
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| LEE, Dong-Soo; YANAGIMOTO UETA, Yoshiko; SUZUKI, Hiroshi; 鈴木, 宏志. |
| In mammals, embryo implantation is an essential step in reproduction. Implantation is a phenomenon that involves crosstalk between the blastocyst and the maternal endometrium. However, the molecular basis of the connections between the blastocyst and endometrium is not yet fully understood. Amphiregulin is a member of the epidermal growth factor family and is known to be expressed in the luminal epithelium of the mouse uterus on 3.5 days post coitum (dpc). Thus, to clarify the mechanism of amphiregulin at fetomaternal interface, we analyzed the expression pattern of amphiregulin mRNA in the oviducts and uteri of pregnant and psuedopregnant mice by means of real-time PCR. Amphiregulin expression in the pregnant uterus dramatically increased on 2.5 dpc,... |
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Palavras-chave: Mice; Implantation; Embryo transfer; Amphiregulin; MRNA. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3026 |
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| ANDRIOLI, A.; SALLES, H. O.; MOURA SOBRINHO, P. A.; SOARES, A. T.; MARQUES, M. A. J.; MORAES, J. B.. |
| Resumo: 0 trabalho tem o objetivo de contribuir corn programas de transferência de embriôes em caprinos no Brasil através do conhecimento das respostas aos tratamentos de sincronizaçao do estro e superovulaçao e da melhor data de colheita de embriôes em relaçao ao estadio embrionario. Os dadas obtidos sao referentes a 19 cabras mestiças das Raças Parda Alpina, Moxoto e Anglo Nubiana (ME), 13 Saanen (SA) e 6 Anglo Nubianas (NB). As doadoras tiveram o estro sincronizado com esponjas vaginais (11 dias) impregnadas corn 60 mg de MAPI, sendo aplicados 50 ug de cloprostenol no nano dia da sincronizaçao. No mesmo dia iniciou-se a superovulaçao das doadoras ME corn 200 mg de NIH-FSH-PI2 e das SA e AN corn 37,5 VI de NIH-FSH.SI3, sendo realizadas 6 aplicaçôes, em... |
| Tipo: Artigo de periódico |
Palavras-chave: Caprino; Transferência de embrião; Goats; Embryo transfer. |
| Ano: 1996 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/514125 |
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| VERGANI, G. B.; ARRAIS, A. M.; MELLO, M. R. B. de; DIAS, J. H.; SOUSA, M. A. P. de; MORAIS, M. C. da C.; FERREIRA, H. C.; BEVILAQUA, J. R.; OLIVEIRA, M. E. F.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da. |
| Abstract: The variability of superovulatory responses is still a limiting factor for the consolidation of commercial embryo transfer programs in small ruminants (Bartlewski et al. 2016. Theriogenology, 86: 130-143). Superovulation protocols are traditionally performed with prior use of progesterone devices for short- or long-term duration (Oliveira et al. 2014. Anim Reprod Sci, 144: 30-37) associated exogenous FSH administration. The presence of dominant follicles in the ovary at the beginning of FSH treatment is detrimental to the growth of the other follicles, in addition, a greater number of antral follicles is required to have a bigger superovulatory response (Fonseca et al., 2010 Acta Sci Vet, 38: 337-369). In this context, the objective of this study... |
| Tipo: Anais e Proceedings de eventos |
Palavras-chave: Ovarian population; População ovariana; Ovino; Ovelha; Progesterona; Superovulação; Reprodução Animal; Transferência de Embrião; Sheep; Animal reproduction; Progesterone; Superovulation; Embryo transfer. |
| Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1112224 |
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| Fonseca,J.F.; Batista,R.I.T.P.; Souza-Fabjan,J.M.G.; Oliveira,M.E.F.; Brandão,F.Z.; Viana,J.H.M.. |
| ABSTRACT The efficiency of an alternative freezing protocol for goat embryos of different morphology and quality was tested. Fifty-eight embryos on Day 6-7 stage were transferred as fresh or after freeze-thawing (n=29/group). For freezing, embryos were placed into 1.5M ethylene-glycol solution for 10min. During this time, they were loaded in the central part of 0.25mL straw, separated by air bubble from columns containing PBS/BSA 0.4% plus 20% BFS. Straws were then frozen using a freezing machine from 20ºC to -6ºC at a cooling rate of 3ºC/min, stabilization for 15min (seeding after 5min), from -6 C to -32ºC at 0.6 C/min,and plunged into liquid nitrogen. Frozen embryos were thawed for 30s at 37ºC in a water bath. Embryos subjected to fresh transfer were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryo survival; Embryo transfer; Goat; Slow freezing. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000501489 |
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| DIAS, J. H.; VERGANI, G. B.; LIMA, M. S. D.; TEIXEIRA, D. I. A.; SILVA, K. de M.; MONTEIRO, A. W. U.; BATISTA, R. I. T. P.; OLIVEIRA, M. E. F.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da. |
| Abstract: Multiple ovulation and embryo transfer (MOET) is one of the most interesting reproductive biotechniques to be used in order to allow a rapid progression in the number of offspring per female. However, it is an underutilized reproductive biotechnology due to the variability of superovulatory response found and the high cost (Baldassare e Karatzas, 2004. AnimReprod Sci, 82/83:255-266). Brazilian naturalized breeds have socio-economic regional importance, but there is no studies reporting the effectiveness of in vivo embryo production on these animals. Thus, the aim of this study was to assess the efficiency of superovulation and viable embryo yield in Canindé and Moxotó goats raised and managed in different places. It is concluded that the in vivo... |
| Tipo: Anais e Proceedings de eventos |
Palavras-chave: Embryo recovery; PIV; Recuperação de embriões; MOET; Caprino; Cabra; Transferência de Embrião; Reprodução Animal; Goats; Embryo transfer; Animal reproduction. |
| Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1112208 |
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| PRELLWITZ, L.; ARRAIS, A. M.; MELLO, M. R. B. de; VERGANI, G. B.; ESTEVES, S. N.; BARBOSA, N. O.; CARVALHO, B. P.; DIAS, J. H.; OLIVEIRA, M. E. F.; FONSECA, J. F. da. |
| Abstract: The first stet for applying superovulation is to know the responsiveness of the ewe to different estrous synchronous protocol duration and possible implications on embryo yield. On the other hand for nonsurgical embryo recovery (NSER) a well consolidated protocol should be tested and, in Morada Nova ewes, successful non-surgical uterine access was reported (Fonseca et al., 2019. Reprod Fertil Devel, 31:17-26). In this context, the objective of this study was to evaluate the efficiency different synchronous estrus induction and NSER as additional tool to evaluate the results these protocols in pluriparous Morada Nova ewes. A total of 18 pluriparous sheep were divided into three treatment groups with 6 (T6, n=6), 9 (T9, n=6) and 12 (T12, n=6) days... |
| Tipo: Anais e Proceedings de eventos |
Palavras-chave: Ovino; Ovelha; Reprodução Animal; Superovulação; Progesterona; Transferência de Embrião; Sheep; Small ruminants; Superovulation; Progesterone; Embryo transfer. |
| Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1112197 |
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| BRAIR, V. L.; PRELLWITZ, L.; CUNHA, R. O.; ZAMBRINI, F. N.; GUIMARÃES, J. D.; SOUSA, M. A. P. de; SOUZA-FABJAN, J. M.; OLIVEIRA, M. E. F. de; FONSECA, J. F. da. |
| Abstract: Ewes have certain particularities in the cervical anatomy leading to the commercial use of surgical methods for collecting embryos in this species. However, the literature shows that Non-Surgical Embryo Recovery (NSER) is possible through specific protocols for cervical relaxation (Fonseca et al., 2019. Reprod Fertil Devel, 31:17-26). The cervical ostium may have several classifications: flap, duckbill, rosette, rose, spiral or papilla. This study aimed to assess the influence of cervical ostium anatomy on the difficulty of traversing cervical rings. In conclusion, the current data suggest that the morphology of cervical ostium may have influence on the success of embryo collection by Non-Surgical Embryo Recovery (NSER) technique. [Influência da... |
| Tipo: Anais e Proceedings de eventos |
Palavras-chave: Embryo recovery; Cervical ostium; Ovino; Ovelha; Transferência de Embrião; Reprodução Animal; Sheep; Embryo transfer. |
| Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1112214 |
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| LIMA, M. S. D.; VERGANI, G. B.; LEITÃO, I. P. A.; SILVA, F. J. C. da; SILVA, K. de M.; MONTEIRO, A. W. U.; PEREIRA, L. M. C.; RAMOS, A. F.; BATISTA, R. I. T. P.; FONSECA, J. F. da; OLIVEIRA, M. E. F.; TEIXEIRA, D. I. A.. |
| The use of color Doppler ultrasonography in multiple ovulation and embryo transfer (MOET) protocols for sheep allows the real?time monitoring of ovarian blood flow and potentially can be a tool to predict embryo production in sheep. Therefore, the present study tested the correlation of ovarian vascularization at 36 h after the end of the superovulation treatment and at 12h (Day 15) before the non?surgical embryo recovery (NSER) (Day 16) with estrus response (ER%), duration of estrus (DE), number of corpus luteum (CL), number of anovulatory follicles (AF), number of structures recovered (SR), number of viable embryos (VE; Grade 1-3). |
| Tipo: Anais e Proceedings de eventos |
Palavras-chave: Sheep; Corpus luteum; Animal reproduction; Embryo transfer; Brazil. |
| Ano: 2020 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1132665 |
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| SANTOS, V. M. B. dos; BALARO, M. F. A.; SANTOS, J. D. R.; TAIRA, A. R.; PEREIRA, P. V. dos S.; GONÇALVES, F. M.; FONSECA, J. F. da; BRANDÃO, F. Z.. |
| Resumo: Métodos para seleção de doadoras de embriões ovinas para a utilização do método não cirúrgico de coleta de embriões já foram demonstrados recentemente (J. R. Santos et al.2019. ReprodDomAnim,00:1-3), entretanto ainda não foi determinado um método que possibilite selecionar receptoras para a transferência transcervical de embriões. Sendo assim, o objetivo desse estudo foi usar o teste de transposição cervical utilizando a vela de Hegar durante o estro visando a seleção de receptoras aptas a passarem pela transferência de embriões não cirúrgica. o teste de vela no estro pode ser adotado em receptoras ovinas de embrião de modo a selecionar fêmeas inovuladas pela via transcervical, sendo as demais destinadas ao procedimento semi-cirúrgico. [Hegar... |
| Tipo: Anais e Proceedings de eventos |
Palavras-chave: Ovelha; Ovino; Reprodução Animal; Transferência de Embrião; Cervix; Sheep; Embryo transfer. |
| Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1112204 |
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| PINTO, P. H. N.; BALARO, M. F. A.; ARASHIRO, E. K. N.; BATISTA, R. I. T. P.; OLIVEIRA, M. E. F.; BRAGANÇA, G. M.; FONSECA, J. F. da; BRANDÃO, F. Z.. |
| Resumo: A presente revisão tem por objetivo abordar os aspectos técnicos em estudos nas diferentes etapas da múltipla ovulação e transferência de embriões em ovinos (MOTE). Apesar dos inúmeros avanços a alta variabilidade de resposta à superovulação ainda é um entrave para esta biotécnica. Visando driblar este gargalo diferentes estratégias têm sido testadas para selecionar fêmeas com melhor potencial de resposta, como a contagem de folículos antrais, pré-seleção com eCG, repetibilidade de resposta, dosagem do hormônio anti-Mülleriano e sincronização da onda folicular. Ajustes de protocolos superovulatórios modificando a frequência, o número de aplicações e a diminuição da dose total de FSH, têm sido testados. Outra tendência é a substituição de... |
| Tipo: Artigo de periódico |
Palavras-chave: Ultrassonografia; Embryo collection; Raça Santa Inês; Ovelha; Ovino; Transferência de embrião; Superovulação; Ultrassom; Reprodução animal; Biotecnologia; Criopreservação; Sheep; Ewes; Embryo transfer; Superovulation; Ultrasonography; Cryopreservation. |
| Ano: 2017 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1069900 |
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| 鈴木, 宏志. |
| Although guide dogs make a remarkable contribution to the quality of life of the blind people, many countries suffer from an acute shortage of guide dogs such that not all those who need them are able to be supplied. One of the important challenges from the point of view of animal and veterinary science as well as social welfare is to establish a stable and effective breeding and reproduction system for guide dogs for the blind. Although the development and extension of assisted reproductive techniques such as artificial insemination, embryo transfer and in vitro fertilization has been remarkable, in canines, it is not a successful as other mammalian spices such as mice, pigs, cows and humans. In this review, recent advances of cryopreservation of sperm,... |
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Palavras-chave: Guide dog; Artifical insemination; Embryo transfer; Recipient; Cryopreservation; イヌ; 人工授精; 移植; 受容雌; 凍結保存. |
| Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4511 |
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| MOURA, R. R.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da; MELO, C. H. S.; SANCHEZ, D. J. D.; VIEIRA, M. P.; ALMEIDA, T. M.; SEROVA, I. A.; SEROV, O. L.; PEREIRA, A. F.; TEIXEIRA, D. I. A.; MELO, L. M.; FREITAS, V. J. F.. |
| Abstract: This study aimed to monitor estrous cycle parameters of a human granulocyte colony-stimulating factor (hG-CSF)-transgenic founder female goat and to perform superovulation and embryo recovery (surgical or transcervical method) for further transfer to recipients to quickly obtain offspring. Two experiments were performed using a transgenic (TF) and a non-transgenic (NTF) female. In experiment 1, three estrous cycles were monitored for the following parameters: estrus behavior, progesterone concentration and ovarian activity. In experiment 2, two superovulation/embryo recovery sessions were performed and the recovered embryos were transferred to previously prepared recipients. Data were compared by either t test or Fisher's exact test. The mean... |
| Tipo: Artigo de periódico |
Palavras-chave: Transgenia; Goat.; Caprino; Cabra; Transferência de embrião; Hormônio animal; Progesterona; Superovulação; Embryo transfer; Progesterone; Superovulation; Transgenesis.. |
| Ano: 2014 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/985516 |
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| Registros recuperados: 61 | |
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