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Detection of rotavirus in dogs with diarrhea in Brazil BJM
Gabbay,Yvone B.; Homem,Valéria S.F.; Munford,Veridiana; Alves,Antonia S.; Mascarenhas,Joana D.P.; Linhares,Alexandre C.; Rácz,Maria Lúcia.
Rotavirus was detected by the enzyme-linked immunosorbent assay (ELISA) in the faeces of a diarrheic dog. Virus particles with morphology typical of rotavirus were visualized by direct electron microscopy. This sample was subsequently tested for the four main human serotypes (G1-G4), by ELISA with monoclonal antibodies. G genotyping was attempted by RT-PCR using G1-G6 and G8-G11 primers but no positive results could be yielded. Also using RT-PCR it was possible to characterize this canine strain as belonging to P[ 3] genotype. This is the first canine rotavirus detected in Brazil.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rotavirus; Diarrheic dog; Genotype G; Genotype P; Belém- Brazil.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100016
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