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Behavior and preservation of an in vitro collection of European aspen in Spain Biocell
Martin,M.T.; Pedranzani,H.E.; Sierra de Grado,R..
An in vitro collection has been established with selected European aspen from Palencia province (Spain). Currently, this collection includes 32 high quality clones, selected for their good bearing and healthy state. Most of them belong to different discrete local populations. Populus tremula L. was propagated in proliferation Aspen Culture Medium; they required subculture every 3 months. The purpose of this study was, therefore, to select a medium which allows the maintenance of 32 clones for a period longer than 3 months without subculture and to observe the behavior of those clones in 15 different culture medium compositions. Seven nodal cutting stem explants from each clone were cultured in parallel in the different media. One and three months after...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Populus tremula; Germplasm conservation; In vitro plant culture; Micropropagation; Selected clone.
Ano: 2007 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000100005
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Inhibitory effect of jasmonic acid and ethylene on epicotyl growth and bud induction in the maritime pine, Pinus pinaster Soland. in Ait Biocell
Martin,Maria Teresa; Pedranzani,Hilda; García-Molinero,Patricia; Pando,Valentin; Sierra-de-Grado,Rosario.
Two independent parameters, epicotyl height (cm) and number of induced buds were studied on Pinus pinaster explants to analyse the effects of three phytohormones (6-benzylaminopurine, jasmonic acid, ethylene) which were combined or not in 11 different treatments. Epicotyle length diminished significantly in relation to the control medium (medium without exogen phytohormones) in presence of jasmonic acid, 6-benzylaminopurine or Ethephon (which is converted to ethylene in plants) in any of treatments. Concentrations of 100 μM of jasmonic acid and Ethephon had a greater inhibitory effect than the treatments with 10 μM. In addition to that, jasmonic acid was a stronger inhibitor than Ethephon in any of the tried combinations. There were no...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Micropropagation; Phytohormones; Plant growth regulators; 6-benzylaminopurine; In vitro plant culture.
Ano: 2009 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000300002
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