| Abstract: Aiming at optimizing the diagnosis of major sugarcane diseases by PCR, primers were developed (scald, orange rust) and amplified fragments according to the literature were used as positive control, such as those caused by: 1 - Bacteria, (ratoon stunting and leaf scald) 2 - Viruses, (yellow leaf, mosaic, mosaic streak and fijivirus) 3-Fungus, (smut, orange rust and curvularia). For diseases with long latency period (leaf scald, ratoon stunting, yellow leaf, mosaic, fijivirus, smut and orange rust), primers were designed for real time PCR. For testing, infected leaves, fungal colonies, amplified DNA fragment or 40 seedlings were used. Real time PCR analyses have enabled the detection of sugarcane samples with highest dilution of DNA. The pair of... |
