Consulta: subjectFacets:"Metalloproteinases"
Registros recuperados: 3
Data/hora: 27/07/2024 02:53:31

Dose-dependent in vitro inhibition of rabbit corneal matrix metalloproteinases by an extract of Pothomorphe umbellata after alkali injury
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Barros,L.F.M.; Barros,P.S.M.; Röpke,C.D.; Silva,V.V.; Sawada,T.C.H.; Barros,S.B.M.; Belfort Jr.,R.
The in vitro ability of Pothomorphe umbellata ethanolic crude extract to inhibit matrix metalloproteinase (MMP) in normal cornea and in cornea after alkali injury was demonstrated. Corneas of albino rabbits were injured with 1 N NaOH for 20 s. After 48 h the corneas were excised, homogenized and analyzed for MMP-9 (92 kDa), pro-MMP-2 (72 kDa) and MMP-2 (67 kDa) activity by gelatin zymography. The activity was also measured in untreated corneas. After electrophoresis of 20 µg protein, gels were incubated with 50, 100, or 250 µg/mL lyophilized hydroethanolic (1:1) root crude extract of P. umbellata standardized for 4-nerolidylcatechol (7.09%). The activity of the enzymes was compared with that of untreated gel. At 48 h after injury, the activity of all MMPs...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cornea; Alkali injury; Metalloproteinases; Pothomorphe umbellata.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800014

Low-dose doxycycline prevents inflammatory bone resorption in rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Bezerra,M.M.; Brito,G.A.C.; Ribeiro,R.A.; Rocha,F.A.C.
Matrix metalloproteinases (MMP) are considered to be key initiators of collagen degradation, thus contributing to bone resorption in inflammatory diseases. We determined whether subantimicrobial doses of doxycycline (DX) (&lt;=10 mg kg-1 day-1), a known MMP inhibitor, could inhibit bone resorption in an experimental periodontitis model. Thirty male Wistar rats (180-200 g) were subjected to placement of a nylon thread ligature around the maxillary molars and sacrificed after 7 days. Alveolar bone loss (ABL) was measured macroscopically in one hemiarcade and the contralateral hemiarcade was processed for histopathologic analysis. Groups of six animals each were treated with DX (2.5, 5 or 10 mg kg-1 day-1, sc, 7 days) and compared to nontreated (NT) rats. NT...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Tetracyclines; Doxycycline; Metalloproteinases; Periodontitis; Osteoporosis; Bone resorption; Inflammation.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500015

Role of laminin bioavailability in the astroglial permissivity for neuritic outgrowth
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: TARDY,MARCIENNE.
The mechanisms involved in the failure of an adult brain to regenerate post-lesion remain poorly understood. The reactive gliosis which occurs after an injury to the CNS and leads to the glial scar has been considered as one of the major impediments to neurite outgrowth and axonal regeneration. A glial scar consists mainly of reactive, hypertrophic astrocytes. These reactive cells acquire new properties, leading to A non-permissive support for neurons. Astrogial reactivity is mainly characteriized by a high overexpression of the major component of the gliofilaments, the glial fibrillary acidic protein (GFAP). This GFAP overexpression is related to the astroglial morphological response to injury. We hypothesized that modulation of GFAP synthesis, reversing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Astrogliosis; Neuro-glia interactions; Neurite outgrowth; Neuronal migration; Laminin; Metalloproteinases.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652002000400009