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Nitric oxide, cholesterol oxides and endothelium-dependent vasodilation in plasma of patients with essential hypertension 56
Moriel,P.; Sevanian,A.; Ajzen,S.; Zanella,M.T.; Plavnik,F.L.; Rubbo,H.; Abdalla,D.S.P..
The objective of the present study was to identify disturbances of nitric oxide radical (·NO) metabolism and the formation of cholesterol oxidation products in human essential hypertension. The concentrations of·NO derivatives (nitrite, nitrate, S-nitrosothiols and nitrotyrosine), water and lipid-soluble antioxidants and cholesterol oxides were measured in plasma of 11 patients with mild essential hypertension (H: 57.8 ± 9.7 years; blood pressure, 148.3 ± 24.8/90.8 ± 10.2 mmHg) and in 11 healthy subjects (N: 48.4 ± 7.0 years; blood pressure, 119.4 ± 9.4/75.0 ± 8.0 mmHg).Nitrite, nitrate and S-nitrosothiols were measured by chemiluminescence and nitrotyrosine was determined by ELISA. Antioxidants were determined by reverse-phase HPLC and cholesterol oxides...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nitrotyrosine; S-nitrosothiols; Nitrate; Oxidized LDL; Lipid peroxidation; Endothelin-1.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100007
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Protein 3-nitrotyrosine formation during Trypanosoma cruzi infection in mice 56
Naviliat,M.; Gualco,G.; Cayota,A.; Radi,R..
Nitric oxide (·NO) is a diffusible messenger implicated in Trypanosoma cruzi resistance. Excess production of ·NO and oxidants leads to the generation of nitrogen dioxide (·NO2), a strong nitrating agent. Tyrosine nitration is a post-translational modification resulting from the addition of a nitro (-NO2) group to the ortho-position of tyrosine residues. Detection of protein 3-nitrotyrosine is regarded as a marker of nitro-oxidative stress and is observed in inflammatory processes. The formation and role of nitrating species in the control and myocardiopathy of T. cruzi infection remain to be studied. We investigated the levels of ·NO and protein 3-nitrotyrosine in the plasma of C3H and BALB/c mice and pharmacologically modulated their production during...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Peroxynitrite; Nitric oxide; Nitrotyrosine; Free radicals; Trypanosoma cruzi; Chagas' disease.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200011
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