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Distribution of pectins in the pollen apertures of Oenothera hookeri.velans ster/+ster: Biocell
Noher de Halac,I.; Cismondi,I.A.; Rodriguez-Garcia,M.I.; Famá,G..
Cell wall pectins are some of the most complex biopolymers known, and yet their functions remain largely mysterious. The aim of this paper was to deepen the study of the spatial pattern of pectin distribution in the aperture of Oenothera hookeri.velans ster/+ster fertile pollen. We used "in situ" immunocytochemical techniques at electron microscopy, involving monoclonal antibodies JIM5 and JIM7 directed against pectin epitopes in fertile pollen grains of Oenothera hookeri.velans ster/+ster. The same region was also analyzed by classical cytochemistry for polysaccharide detection. Immunogold labelling at the JIM7 epitope showed only in mature pollen labelling mainly located at the intine endo-aperture region. Cytoplasmic structures near the plasma membrane...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pectins; Monoclonal antibodies; JIM5; JIM7; Immunogold; Pollen; Sporoderm; Oenothera; Cytochemistry.
Ano: 2003 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000100002
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Acidic polysaccharides from Psidium cattleianum (Araçá) BABT
Vriesmann,Lúcia Cristina; Petkowicz,Carmen Lúcia de Oliveira; Carneiro,Paulo Irajara Borba; Costa,Maria Eugênia; Beleski-Carneiro,Eliana.
The aim of this work was to study the acidic polysaccharides of Psidium cattleianum. Pectic fractions were obtained by successive extractions with water, EDTA and sodium carbonate (4 and 25ºC). Monosaccharide composition, total carbohydrates, uronic acids and protein contents were determined for each fraction. The yield of water and EDTA-soluble fractions and high content of uronic acid were consistent with the presence of pectins probably arising from the middle lamellae. On the other hand, sodium carbonate-soluble pectins had a higher neutral sugar content, indicating highly branched polysaccharides in these fractions, consistent with pectins from cell wall.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pectins; Acidic polysaccharides; Psidium cattleianum; Cell wall.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200001
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Contenido y caracterización reológica de los polisacáridos estructurales de nopalitos (Opuntia spp.) en un gradiente de domesticación. Colegio de Postgraduados
López Palacios, Cristian.
Evidencias diversas indican que la domesticación del género Opuntia comenzó con la selección de variantes de O. streptacantha, seguida de O. hyptiacantha y O. megacantha y se centró en O. albicarpa y O. ficus indica. Las causas por las cuales se domesticó Opuntia fueron por sus frutos y nopalitos. Los polisacáridos más abundantes en los nopalitos son del tipo estructural. La hipótesis que se plantea es que los polisacáridos muestran un gradiente de concentración y sus propiedades reológicas han sido modificadas en función del grado de domesticación de Opuntia. Los polisacáridos estructurales fueron extraídos de cladodios jóvenes de 14 variantes pertenecientes a especies representantes del gradiente de domesticación (O. streptacantha, O. hyptiacantha, O....
Palavras-chave: Opuntia; Gradiente de domesticación; Reología; Fibra alimentaria; Mucílagos; Pectinas; Hemicelulosas; Celulosa; Domestication gradient; Rheology; Dietary fiber; Mucilages; Pectins; Hemicelluloses; Cellulose; Maestría; Botánica.
Ano: 2011 URL: http://hdl.handle.net/10521/354
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