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Effects of insulin and actin on phosphofructokinase activity and cellular distribution in skeletal muscle Anais da ABC (AABC)
Silva,Ana Paula P.; Alves,Gutemberg G.; Araújo,Alexandre H.B.; Sola-Penna,Mauro.
In this work, we report evidences that the association of phosphofructokinase and F-actin can be affected by insulin stimulation in rabbit skeletal muscle homogenates and that this association can be a mechanism of phos-phofructokinase regulation. Through co-sedimentation techniques, we observed that on insulin-stimulated tissues, approximately 70% of phosphofructokinase activity is co-located in an actin-enriched fraction, against 28% in control. This phenomenon is accompanied by a 100% increase in specific phosphofructokinase activity in stimulated homogenates. Purified F-actin causes an increase of 230% in phosphofructokinase activity and alters its kinetic parameters. The presence of F-actin increases the affinity of phosphofructokinase for fructose...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phosphofructokinase; Hormone; Rabbit; Actin; Metabolism; Glycolysis.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652004000300008
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Allosteric regulation of 6-phosphofructo-1-kinase activity of fat body and flight muscle from the bloodsucking bug Rhodnius prolixus Anais da ABC (AABC)
Alves,Gutemberg G.; Marinho-Carvalho,Monica M.; Atella,Georgia C.; Silva-Neto,Mario A.C.; Sola-Penna,Mauro.
6-phosphofructo-1-kinase (phosphofructokinase; PFK) activity from Rhodnius prolixus, a haematophagous insect which is usually a poor flyer, was measured and compared in two metabolically active tissues - flight muscle and fat body. The activity of this important regulatory glycolytic enzyme was much more pronounced in muscle (15.1 ± 1.4 U/mg) than in fat body extracts (3.6±0.4 U/mg), although the latter presented higher levels of enzyme per protein content, as measured by western-blotting. Muscle extracts are more responsible than fat body to ATP and fructose 6-phosphate, both substrates of PFK. Allosteric regulation exerted by different effectors such as ADP, AMP and fructose 2,6-phosphate presented a singular pattern for each tissue. Optimal pH (8.0-8.5)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phosphofructokinase; Metabolism; Insect.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652007000100008
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