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| Pillai,Aneesh Balakrishna; Kumar,Arjun Jaya; Thulasi,Kavitha; Kumarapillai,Harikrishnan. |
| Abstract This study was focused on the polyhydroxybutyrate (PHB) accumulation property of Bacillus aryabhattai isolated from environment. Twenty-four polyhydroxyalkanoate (PHA) producers were screened out from sixty-two environmental bacterial isolates based on Sudan Black B colony staining. Based on their PHA accumulation property, six promising isolates were further screened out. The most productive isolate PHB10 was identified as B. aryabhattai PHB10. The polymer production maxima were 3.264 g/L, 2.181 g/L, 1.47 g/L, 1.742 g/L and 1.786 g/L in glucose, fructose, maltose, starch and glycerol respectively. The bacterial culture reached its stationary and declining phases at 18 h and 21 h respectively and indicated growth-associated PHB production. Nuclear... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacillus aryabhattai PHB10; Polyhydroxybutyrate; Domestic sewerage; Polymer characterization; 16S rRNA. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300451 |
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| Sangkharak,Kanokphorn; Prasertsan,Poonsuk. |
| Three halotolerant bacterial strains; Rhodobacter sphaeroides ES16 (the wild type) and the two mutant strains of R. sphaeroides ES16, namely N20 and U7, were cultivated in glutamate-malate (GM) medium and screened for production of polyhydroxybutyrate (PHB). The mutant strains N20 and U7 were found to accumulate PHB (53.9 and 42.0% of DCW, respectively) 3.6 and 2.8 times higher than the wild type strain (19.5% of DCW), respectively. R. sphaeroides N20 were selected for studies on the effects of nutrient and environmental conditions on PHB accumulation. The optimal condition was 4 g/l acetate, 0.02 g/l (NH4)2SO4, C/N ratio of 6:1, 1.0 g/l K2HPO4, 1.0 g/l KH2PO4 and 3% NaCl with initial pH at 7.0. Under this optimal condition, the maximum PHB accumulation... |
| Tipo: Journal article |
Palavras-chave: Aerobic-dark cultivation; Optimization; PHB; Polyhydroxybutyrate; Rhodobacter sphaeroides. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300009 |
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| Ramadas,Nisha V.; Singh,Sudheer Kumar; Soccol,Carlos Ricardo; Pandey,Ashok. |
| The aim of this work was to study the production of polyhydroxybutyrate (PHB) using agro- industrial residues as the carbon source. Seven substrates, viz., wheat bran, potato starch, sesame oil cake, groundnut oil cake, cassava powder, jackfruit seed powder and corn flour were hydrolyzed using commercial enzymes and the hydrolyzates assessed for selecting the best substrate for PHB production. Jackfruit seed powder gave the maximum production of PHB under submerged fermentation using Bacillus sphaericus (19%) at the initial pH of 7.5. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacillus sphaericus; Polyhydroxybutyrate; Jackfruit seed powder. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100003 |
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