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Bone marrow fibroblasts in patients with advanced lung cancer BJMBR
Chasseing,N.A.; Hofer,E.; Bordenave,R.H.; Shanley,C.; Rumi,L.S..
In a previous study we demonstrated that the incidence of fibroblast colony-forming units (CFU-F) was very low in bone marrow primary cultures from the majority of untreated advanced non-small lung cancer patients (LCP) compared to normal controls (NC). For this reason, we studied the ability of bone marrow stromal cells to achieve confluence in primary cultures and their proliferative capacity following four continuous subcultures in consecutive untreated LCP and NC. We also evaluated the production of interleukin-1ß (IL-1ß) and prostaglandin E2 (PGE2) by pure fibroblasts. Bone marrow was obtained from 20 LCP and 20 NC. A CFU-F assay was used to investigate the proliferative and confluence capacity. Levels of IL-1ß and PGE2 in conditioned medium (CM) of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fibroblasts; Interleukin-1; Prostaglandin E2; Cancer.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100014
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A novel hot-plate test sensitive to hyperalgesic stimuli and non-opioid analgesics BJMBR
Lavich,T.R.; Cordeiro,R.S.B.; Silva,P.M.R.; Martins,M.A..
It is widely accepted that the classical constant-temperature hot-plate test is insensitive to cyclooxygenase inhibitors. In the current study, we developed a variant of the hot-plate test procedure (modified hot-plate (MHP) test) to measure inflammatory nociception in freely moving rats and mice. Following left and right hind paw stimulation with a phlogogen and vehicle, respectively, the animals were placed individually on a hot-plate surface at 51ºC and the withdrawal latency for each paw was determined simultaneously in measurements performed at 15, 60, 180, and 360 min post-challenge. Plantar stimulation of rats (250 and 500 µg/paw) and mice (125-500 µg/paw) with carrageenan led to a rapid hyperalgesic response of the ipsilateral paw that reached a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hyperalgesia; Hargreaves' test; Modified hot-plate test; Carrageenan; Prostaglandin E2; Indomethacin.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300016
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Arachidonic acid affects biofilm formation and PGE2 level in Candida albicans and non-albicans species in presence of subinhibitory concentration of fluconazole and terbinafine BJID
Mishra,Nripendra Nath; Ali,Shakir; Shukla,Praveen K..
Candida albicans utilizes arachidonic acid (AA) released during the course of infection (Candidiasis) from phospholipids of infected host cell membranes and synthesizes extracellular prostaglandin(s) which play an important role in hyphae formation and host cell damage. C. albicans biofilms secrete significantly more prostaglandin(s) and evidence suggests that Candida biofilms have dramatically reduced susceptibility to majority of antifungal drugs. AA influences the saturation level of lipids and fluidity of yeast cell membranes. Therefore the aim of this study was to evaluate the effect of AA alone or in combination with antifungal agents on biofilm formation and production of prostaglandin (PGE2) in C. albicans, C. parapsilosis, C. glabrata, C....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arachidonic acid; Candida albicans; AmBR; Candida tropicalis; Prostaglandin E2; FDA.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000300287
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Effects of meloxicam administered by different routes to control experimental uveitis in dogs Ciência Rural
Ribeiro,Alexandre Pinto; Escobar,André; Motheo,Tathiana Fergunson; Godoy,Guilherme Selera; Laus,José Luiz.
Efficacy of meloxicam, administered by different routes was studied in experimental uveitis in dogs. Anterior chamber paracenteses was accomplished at two different moments (M0 and M1), with a five hour interval among them. At M0 and M1, 0.2mL of aqueous humor was collected and total protein and prostaglandin E2 (PGE2) quantitation was determined. Four groups were formed (n=5), which received meloxicam at the end of M0. by the following routes: subcutaneous (GIm), subconjunctival (GII), and topical (GIII). A fourth group that received no treatment was instituted (Control). Conjunctival histopathology of the GII was performed. Results were evaluated statistically (P≤0.05). In all groups, protein and PGE2 values enhanced significantly in M1. Protein and PGE2...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Uveitis; Meloxicam; Total protein; Prostaglandin E2; Dogs.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000700024
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Effects of carprofen administered by different routes to control experimental uveitis in dogs Arq. Bras. Med. Vet. Zootec.
Ribeiro,A.P.; Escobar,A.; Motheo,T.F.; Sakamoto,C.A.M.; Godoy,G.S.; Costa,M.A.N.; Laus,J.L..
Efficacy of carprofen, administered by different routes, was studied in experimental uveitis in dogs. Anterior chamber paracenteses was accomplished at two different moments (M0 and M1), with a five hour interval between them. At M0 and M1, 0.2mL of aqueous humor was collected and quantitation of total protein and prostaglandin E2 (PGE2) were determined. Four groups were formed (n=8), which received carprofen at the end of M0, by the following routes: subcutaneous (GIm), subconjunctival (GII), and topical (GIII). A fourth group that received no treatment was instituted (Control). Conjunctival histopathology of the GII animals was performed. In all groups, values of protein and PGE2 significantly enhanced at M1; however, they did not significantly change...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dog; Uveitis; Carprofen; Total protein; Prostaglandin E2.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000200010
Registros recuperados: 5
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